Helianthus annuus (Common sunflower)
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 51240 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A251TI58|A0A251TI58_HELAN Fatty acyl-CoA reductase OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr10g0291761 PE=3 SV=1
MM1 pKa = 7.62 DD2 pKa = 4.15 TSDD5 pKa = 4.22 TGDD8 pKa = 3.3 SDD10 pKa = 3.94 TTGPLPMVSDD20 pKa = 5.27 DD21 pKa = 3.61 IVSSEE26 pKa = 4.59 HH27 pKa = 6.11 EE28 pKa = 4.49 VHH30 pKa = 6.09 TSDD33 pKa = 4.6 YY34 pKa = 10.96 TSTDD38 pKa = 3.38 DD39 pKa = 6.61 DD40 pKa = 4.43 DD41 pKa = 4.69 FQPFALPDD49 pKa = 3.92 GADD52 pKa = 3.58 EE53 pKa = 5.43 PIVGDD58 pKa = 3.76 PAEE61 pKa = 4.78 DD62 pKa = 3.69 LPLVVIPAPIPLASYY77 pKa = 8.76 PAYY80 pKa = 10.12 EE81 pKa = 4.38 LMPGAEE87 pKa = 4.38 ADD89 pKa = 3.49 GDD91 pKa = 3.51 IDD93 pKa = 4.81 LFEE96 pKa = 5.54 DD97 pKa = 4.15 EE98 pKa = 4.77 PLEE101 pKa = 5.43 DD102 pKa = 4.29 EE103 pKa = 4.87 VPDD106 pKa = 4.43 PALLPAGGLLMIAGAPVGDD125 pKa = 4.49 SPVHH129 pKa = 6.02 SPVPDD134 pKa = 3.35 SCEE137 pKa = 4.14 SVASAPSHH145 pKa = 5.38 EE146 pKa = 4.47 VSIQHH151 pKa = 6.07 FVHH154 pKa = 7.63 DD155 pKa = 4.98 SDD157 pKa = 4.74 PDD159 pKa = 3.67 QASSAAPIPSLVFEE173 pKa = 4.97 HH174 pKa = 7.42 DD175 pKa = 4.07 VIEE178 pKa = 5.08 DD179 pKa = 3.7 SDD181 pKa = 4.25 PVFPPGFDD189 pKa = 3.49 PDD191 pKa = 3.91 RR192 pKa = 11.84 DD193 pKa = 3.48 IEE195 pKa = 4.54 YY196 pKa = 10.37 IPMDD200 pKa = 3.59 HH201 pKa = 7.42 HH202 pKa = 6.67 MEE204 pKa = 4.64 DD205 pKa = 3.71 PVDD208 pKa = 3.92 PVDD211 pKa = 5.27 PIDD214 pKa = 5.58 PIDD217 pKa = 4.22 PDD219 pKa = 4.25 FDD221 pKa = 4.85 FDD223 pKa = 4.14 MAFDD227 pKa = 4.31 DD228 pKa = 4.94 PEE230 pKa = 4.22 PAVAPEE236 pKa = 3.55 QAAAFDD242 pKa = 4.42 PVHH245 pKa = 6.1 EE246 pKa = 5.36 HH247 pKa = 7.17 DD248 pKa = 5.21 LVHH251 pKa = 7.17 ADD253 pKa = 3.63 VPVDD257 pKa = 3.75 PVLADD262 pKa = 3.88 PPVGDD267 pKa = 4.84 LPVDD271 pKa = 4.06 DD272 pKa = 5.29 VPLLADD278 pKa = 3.89 DD279 pKa = 4.71 HH280 pKa = 7.29 VVDD283 pKa = 5.1 PFVDD287 pKa = 4.05 PPLIADD293 pKa = 3.73 VPVDD297 pKa = 3.15 HH298 pKa = 7.13 HH299 pKa = 7.22 VDD301 pKa = 3.21 HH302 pKa = 7.23 VDD304 pKa = 3.41 PVIAPFDD311 pKa = 3.84 LVPIEE316 pKa = 4.54 PEE318 pKa = 3.48 HH319 pKa = 7.75 ALFAEE324 pKa = 4.81 HH325 pKa = 7.04 MDD327 pKa = 4.15 PPDD330 pKa = 3.72 EE331 pKa = 4.27 EE332 pKa = 5.14 AQHH335 pKa = 5.62 GWIPADD341 pKa = 3.65 EE342 pKa = 5.0 DD343 pKa = 4.16 VPPLPPHH350 pKa = 5.9 HH351 pKa = 7.0 TEE353 pKa = 3.48 AHH355 pKa = 5.63 HH356 pKa = 6.69 TDD358 pKa = 3.86 FSFQIPPFVPPAGPGEE374 pKa = 4.43 GSSAHH379 pKa = 6.41 PFGHH383 pKa = 6.65 IPMPMPFMPQMTPVPSSVHH402 pKa = 5.12 VAPFDD407 pKa = 3.78 PTSTPLFWSSSSPMPPTDD425 pKa = 3.98 PYY427 pKa = 11.15 HH428 pKa = 6.48 PFHH431 pKa = 6.83 MGHH434 pKa = 6.67 TIEE437 pKa = 4.87 DD438 pKa = 3.55 VLMSFVHH445 pKa = 5.27 QHH447 pKa = 6.73 DD448 pKa = 3.88 SHH450 pKa = 6.0 SQRR453 pKa = 11.84 LQEE456 pKa = 4.53 LEE458 pKa = 3.86 RR459 pKa = 11.84 AQLSFGPYY467 pKa = 10.1 LGQTSSSFQPFRR479 pKa = 11.84 SLPPDD484 pKa = 3.8 FAARR488 pKa = 11.84 LTTMEE493 pKa = 3.94 QQIASVICTQQAMEE507 pKa = 4.69 EE508 pKa = 4.25 DD509 pKa = 3.87 WLHH512 pKa = 6.42 LRR514 pKa = 11.84 RR515 pKa = 11.84 LLYY518 pKa = 9.72 THH520 pKa = 7.28 FPPPPPPSAA529 pKa = 3.84
Molecular weight: 57.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.686
IPC2_protein 3.783
IPC_protein 3.834
Toseland 3.605
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.656
Grimsley 3.503
Solomon 3.821
Lehninger 3.783
Nozaki 3.935
DTASelect 4.215
Thurlkill 3.656
EMBOSS 3.783
Sillero 3.961
Patrickios 1.532
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.824
Protein with the highest isoelectric point:
>tr|A0A251SQ45|A0A251SQ45_HELAN Putative UDP-glucuronosyl/UDP-glucosyltransferase OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr13g0393321 PE=3 SV=1
MM1 pKa = 7.16 QLRR4 pKa = 11.84 GKK6 pKa = 9.97 LVLVGRR12 pKa = 11.84 RR13 pKa = 11.84 VPGARR18 pKa = 11.84 VLATLKK24 pKa = 10.45 LARR27 pKa = 11.84 LRR29 pKa = 11.84 LSQRR33 pKa = 11.84 MKK35 pKa = 8.71 RR36 pKa = 11.84 WKK38 pKa = 10.32 RR39 pKa = 11.84 KK40 pKa = 8.54 MPLPNLLGGRR50 pKa = 11.84 GVGARR55 pKa = 11.84 LQLLSLPRR63 pKa = 11.84 LHH65 pKa = 6.93 LWW67 pKa = 3.33
Molecular weight: 7.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
51240
0
51240
17848194
50
5399
348.3
39.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.115 ± 0.011
1.919 ± 0.006
5.358 ± 0.009
6.076 ± 0.011
4.397 ± 0.008
6.385 ± 0.011
2.513 ± 0.006
5.579 ± 0.01
6.258 ± 0.011
9.664 ± 0.013
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.568 ± 0.005
4.716 ± 0.008
4.785 ± 0.012
3.546 ± 0.007
5.115 ± 0.008
8.513 ± 0.013
5.319 ± 0.007
6.797 ± 0.01
1.338 ± 0.005
3.032 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here