Staphylococcus phage UPMK_2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Phietavirus; Staphylococcus virus UPMK2

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I6PCT8|A0A2I6PCT8_9CAUD Uncharacterized protein OS=Staphylococcus phage UPMK_2 OX=2029631 GN=CLI63_00014 PE=4 SV=1
MM1 pKa = 7.28GCLVVVKK8 pKa = 10.07EE9 pKa = 3.89ILRR12 pKa = 11.84LLFLLAMYY20 pKa = 10.11EE21 pKa = 3.89LGNYY25 pKa = 6.9VTEE28 pKa = 4.03QVYY31 pKa = 10.94IMMTANDD38 pKa = 4.14DD39 pKa = 3.74VEE41 pKa = 5.03APSDD45 pKa = 3.89FPKK48 pKa = 11.02LSDD51 pKa = 3.55QYY53 pKa = 11.73DD54 pKa = 3.67LMRR57 pKa = 11.84AEE59 pKa = 4.2VSEE62 pKa = 4.21

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I6PCT1|A0A2I6PCT1_9CAUD Uncharacterized protein OS=Staphylococcus phage UPMK_2 OX=2029631 GN=CLI63_00005 PE=4 SV=1
MM1 pKa = 7.32NKK3 pKa = 9.57ILIRR7 pKa = 11.84FAINYY12 pKa = 8.84IKK14 pKa = 10.44YY15 pKa = 8.01QQKK18 pKa = 8.41QLRR21 pKa = 11.84EE22 pKa = 3.59KK23 pKa = 9.95EE24 pKa = 3.86ARR26 pKa = 11.84IKK28 pKa = 10.67YY29 pKa = 10.51LEE31 pKa = 4.46GFLKK35 pKa = 10.83GKK37 pKa = 10.62GYY39 pKa = 11.17

Molecular weight: 4.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12847

39

989

207.2

23.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.822 ± 0.415

0.529 ± 0.093

6.546 ± 0.275

7.34 ± 0.52

4.219 ± 0.204

5.877 ± 0.39

1.814 ± 0.154

7.317 ± 0.245

9.107 ± 0.458

7.426 ± 0.246

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.475 ± 0.177

6.57 ± 0.254

2.794 ± 0.198

4.079 ± 0.23

4.102 ± 0.267

5.768 ± 0.34

6.235 ± 0.264

6.282 ± 0.284

1.253 ± 0.156

4.445 ± 0.338
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski