Thermaerobacter marianensis (strain ATCC 700841 / DSM 12885 / JCM 10246 / 7p75a)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Clostridiales Family XVII. Incertae Sedis; Thermaerobacter; Thermaerobacter marianensis

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2324 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E6SGA4|E6SGA4_THEM7 Uncharacterized protein OS=Thermaerobacter marianensis (strain ATCC 700841 / DSM 12885 / JCM 10246 / 7p75a) OX=644966 GN=Tmar_0400 PE=4 SV=1
MM1 pKa = 7.21YY2 pKa = 10.07PEE4 pKa = 4.74EE5 pKa = 3.78YY6 pKa = 9.82RR7 pKa = 11.84YY8 pKa = 10.7AKK10 pKa = 9.78WPEE13 pKa = 3.53AYY15 pKa = 8.01ATNFPGTAYY24 pKa = 11.04LNTQASDD31 pKa = 4.33DD32 pKa = 3.87EE33 pKa = 4.77TEE35 pKa = 4.34EE36 pKa = 3.83NWTVGTLDD44 pKa = 3.36AGEE47 pKa = 4.31FEE49 pKa = 5.65PYY51 pKa = 8.14VQYY54 pKa = 10.9YY55 pKa = 10.46ADD57 pKa = 4.55IIGSPDD63 pKa = 3.36YY64 pKa = 11.04PGTNWWKK71 pKa = 10.59LYY73 pKa = 10.73FEE75 pKa = 5.52LGVIDD80 pKa = 5.79APLCGITVSPEE91 pKa = 2.99WCMLYY96 pKa = 10.27PHH98 pKa = 7.26DD99 pKa = 4.84RR100 pKa = 11.84YY101 pKa = 10.25MRR103 pKa = 11.84IPFTYY108 pKa = 9.62HH109 pKa = 3.62YY110 pKa = 7.4TFPGGIYY117 pKa = 9.77WYY119 pKa = 10.27GQQ121 pKa = 3.01

Molecular weight: 14.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E6SM33|E6SM33_THEM7 Phosphoglucosamine mutase OS=Thermaerobacter marianensis (strain ATCC 700841 / DSM 12885 / JCM 10246 / 7p75a) OX=644966 GN=glmM PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.56RR3 pKa = 11.84TFQPNRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.49RR14 pKa = 11.84THH16 pKa = 6.5GFLVRR21 pKa = 11.84MRR23 pKa = 11.84TKK25 pKa = 10.61GGRR28 pKa = 11.84KK29 pKa = 8.28VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.27GRR39 pKa = 11.84HH40 pKa = 4.74RR41 pKa = 11.84LAVV44 pKa = 3.37

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2324

0

2324

797153

31

1706

343.0

36.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.196 ± 0.087

0.657 ± 0.013

4.864 ± 0.037

5.98 ± 0.049

2.705 ± 0.03

10.625 ± 0.064

1.908 ± 0.021

3.435 ± 0.038

1.648 ± 0.03

10.484 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.784 ± 0.021

1.55 ± 0.021

7.02 ± 0.059

3.125 ± 0.033

9.369 ± 0.054

3.542 ± 0.028

4.563 ± 0.032

8.852 ± 0.049

1.612 ± 0.026

2.08 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski