Streptomycetaceae bacterium MP113-05

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; unclassified Streptomycetaceae

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5132 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V6L7T9|V6L7T9_9ACTN DDE_3 domain-containing protein OS=Streptomycetaceae bacterium MP113-05 OX=1380770 GN=N566_00410 PE=4 SV=1
MM1 pKa = 7.02STGNEE6 pKa = 3.93ALEE9 pKa = 3.98VWIDD13 pKa = 3.41QDD15 pKa = 3.99LCTGDD20 pKa = 5.01GICAQYY26 pKa = 10.9APEE29 pKa = 4.23VFEE32 pKa = 5.9LDD34 pKa = 3.05IDD36 pKa = 3.71GLAYY40 pKa = 10.84VKK42 pKa = 10.83DD43 pKa = 3.96SGDD46 pKa = 3.73EE47 pKa = 3.92LLQDD51 pKa = 4.1KK52 pKa = 10.42GATAPVPLPLLKK64 pKa = 10.43DD65 pKa = 3.69VQASAVEE72 pKa = 4.29CPGEE76 pKa = 4.45CIHH79 pKa = 6.3VRR81 pKa = 11.84RR82 pKa = 11.84VSDD85 pKa = 3.41GAEE88 pKa = 3.7VHH90 pKa = 6.79GPDD93 pKa = 5.01AEE95 pKa = 4.16

Molecular weight: 10.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V6KZP9|V6KZP9_9ACTN Glyoxalase_6 domain-containing protein OS=Streptomycetaceae bacterium MP113-05 OX=1380770 GN=N566_15860 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.94GRR40 pKa = 11.84TRR42 pKa = 11.84VSAA45 pKa = 3.75

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5132

0

5132

1561553

29

3387

304.3

32.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.137 ± 0.05

0.841 ± 0.009

6.146 ± 0.026

6.058 ± 0.032

2.725 ± 0.019

9.675 ± 0.032

2.462 ± 0.018

2.844 ± 0.023

1.963 ± 0.029

10.39 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.786 ± 0.014

1.667 ± 0.015

6.014 ± 0.032

2.75 ± 0.019

8.407 ± 0.034

5.071 ± 0.021

6.001 ± 0.024

8.629 ± 0.031

1.447 ± 0.013

1.988 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski