Thermoplasmatales archaeon B_DKE

Taxonomy: cellular organisms; Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales; unclassified Thermoplasmatales

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1928 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A218ZW38|A0A218ZW38_9ARCH Uncharacterized protein OS=Thermoplasmatales archaeon B_DKE OX=1961136 GN=B2I17_03595 PE=4 SV=1
MM1 pKa = 7.45SFDD4 pKa = 4.12DD5 pKa = 5.61ANLFDD10 pKa = 6.27LMDD13 pKa = 4.17SCQSLGDD20 pKa = 3.68TRR22 pKa = 11.84FGGSGTRR29 pKa = 11.84DD30 pKa = 2.91EE31 pKa = 5.93DD32 pKa = 3.56ILVGYY37 pKa = 9.81IYY39 pKa = 10.99GVLSEE44 pKa = 4.36SASTEE49 pKa = 3.8LLYY52 pKa = 9.62DD53 pKa = 3.62TKK55 pKa = 10.85LAKK58 pKa = 10.04AYY60 pKa = 9.77KK61 pKa = 8.51YY62 pKa = 10.85GEE64 pKa = 4.13YY65 pKa = 10.5SYY67 pKa = 9.22MVWMGEE73 pKa = 4.06FEE75 pKa = 4.55LEE77 pKa = 4.12EE78 pKa = 4.79SGEE81 pKa = 4.03QDD83 pKa = 4.75DD84 pKa = 4.97EE85 pKa = 4.68PLVLPVAVEE94 pKa = 4.5GPFRR98 pKa = 11.84DD99 pKa = 4.15GEE101 pKa = 3.99IEE103 pKa = 4.4EE104 pKa = 4.52ILKK107 pKa = 10.19QLL109 pKa = 3.85

Molecular weight: 12.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A218ZTV9|A0A218ZTV9_9ARCH AB hydrolase-1 domain-containing protein OS=Thermoplasmatales archaeon B_DKE OX=1961136 GN=B2I17_03720 PE=4 SV=1
MM1 pKa = 7.23KK2 pKa = 10.23RR3 pKa = 11.84SSRR6 pKa = 11.84DD7 pKa = 2.69WKK9 pKa = 10.88KK10 pKa = 10.3RR11 pKa = 11.84GNMRR15 pKa = 11.84WKK17 pKa = 8.95WRR19 pKa = 11.84KK20 pKa = 9.27KK21 pKa = 9.47RR22 pKa = 11.84IRR24 pKa = 11.84RR25 pKa = 11.84LKK27 pKa = 10.24RR28 pKa = 11.84ARR30 pKa = 11.84KK31 pKa = 7.91ANKK34 pKa = 8.37MM35 pKa = 3.64

Molecular weight: 4.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1928

0

1928

536744

29

2108

278.4

31.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.506 ± 0.048

0.66 ± 0.018

5.137 ± 0.05

6.223 ± 0.066

4.736 ± 0.047

7.52 ± 0.051

1.634 ± 0.025

8.41 ± 0.054

6.078 ± 0.063

8.978 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.933 ± 0.028

4.601 ± 0.059

3.831 ± 0.039

2.358 ± 0.031

5.136 ± 0.055

8.122 ± 0.069

5.289 ± 0.061

7.035 ± 0.044

0.868 ± 0.018

3.944 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski