Streptococcus phage Javan173
Average proteome isoelectric point is 5.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6A291|A0A4D6A291_9CAUD HTH cro/C1-type domain-containing protein OS=Streptococcus phage Javan173 OX=2548022 GN=Javan173_0051 PE=4 SV=1
MM1 pKa = 7.57 EE2 pKa = 6.41 LLLQSLQRR10 pKa = 11.84 WNATGDD16 pKa = 3.49 EE17 pKa = 4.15 EE18 pKa = 5.92 HH19 pKa = 6.68 YY20 pKa = 10.95 QDD22 pKa = 4.15 LQRR25 pKa = 11.84 ILDD28 pKa = 3.86 YY29 pKa = 11.44 QEE31 pKa = 5.41 GEE33 pKa = 4.3 EE34 pKa = 4.24 NGCC37 pKa = 4.18
Molecular weight: 4.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.858
IPC2_protein 4.139
IPC_protein 3.923
Toseland 3.77
ProMoST 4.075
Dawson 3.884
Bjellqvist 4.05
Wikipedia 3.795
Rodwell 3.77
Grimsley 3.706
Solomon 3.859
Lehninger 3.808
Nozaki 4.037
DTASelect 4.113
Thurlkill 3.821
EMBOSS 3.808
Sillero 4.037
Patrickios 1.901
IPC_peptide 3.859
IPC2_peptide 4.024
IPC2.peptide.svr19 3.949
Protein with the highest isoelectric point:
>tr|A0A4D6A672|A0A4D6A672_9CAUD Portal protein OS=Streptococcus phage Javan173 OX=2548022 GN=Javan173_0023 PE=4 SV=1
MM1 pKa = 6.78 VRR3 pKa = 11.84 QMAYY7 pKa = 9.84 KK8 pKa = 10.3 SKK10 pKa = 9.14 QAQVATEE17 pKa = 4.24 KK18 pKa = 10.56 EE19 pKa = 4.31 LEE21 pKa = 4.09 RR22 pKa = 11.84 SSLRR26 pKa = 11.84 IEE28 pKa = 4.43 RR29 pKa = 11.84 SAKK32 pKa = 9.91 VNAPVKK38 pKa = 10.55 KK39 pKa = 10.13 GVLRR43 pKa = 11.84 NTIFSIKK50 pKa = 10.07 AGKK53 pKa = 8.03 LTYY56 pKa = 10.35 KK57 pKa = 9.19 VTAPQHH63 pKa = 4.35 YY64 pKa = 9.9 AIYY67 pKa = 10.09 VEE69 pKa = 4.0 KK70 pKa = 9.85 GTRR73 pKa = 11.84 KK74 pKa = 8.61 MRR76 pKa = 11.84 AKK78 pKa = 10.47 PFMAPAIDD86 pKa = 3.63 AEE88 pKa = 4.3 RR89 pKa = 11.84 PKK91 pKa = 10.84 LINNLRR97 pKa = 11.84 KK98 pKa = 9.65 IYY100 pKa = 10.53 GKK102 pKa = 10.52
Molecular weight: 11.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.263
IPC2_protein 9.97
IPC_protein 10.409
Toseland 10.847
ProMoST 10.438
Dawson 10.935
Bjellqvist 10.555
Wikipedia 11.082
Rodwell 11.447
Grimsley 10.965
Solomon 10.994
Lehninger 10.965
Nozaki 10.804
DTASelect 10.555
Thurlkill 10.818
EMBOSS 11.228
Sillero 10.847
Patrickios 11.169
IPC_peptide 10.994
IPC2_peptide 9.136
IPC2.peptide.svr19 8.544
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
57
0
57
13120
37
1550
230.2
25.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.399 ± 0.766
0.473 ± 0.092
6.128 ± 0.457
7.088 ± 0.563
3.986 ± 0.221
6.7 ± 0.453
1.479 ± 0.178
6.242 ± 0.282
7.325 ± 0.403
8.346 ± 0.313
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.462 ± 0.177
5.152 ± 0.252
2.858 ± 0.247
4.802 ± 0.238
4.337 ± 0.371
6.38 ± 0.56
6.646 ± 0.354
6.829 ± 0.229
1.09 ± 0.146
3.277 ± 0.337
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here