Amino acid dipepetide frequency for Mycobacterium phage Onyinye

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.071AlaAla: 12.071 ± 1.075
0.868AlaCys: 0.868 ± 0.244
5.645AlaAsp: 5.645 ± 0.44
6.73AlaGlu: 6.73 ± 0.93
3.387AlaPhe: 3.387 ± 0.516
7.034AlaGly: 7.034 ± 0.825
1.563AlaHis: 1.563 ± 0.26
4.299AlaIle: 4.299 ± 0.487
6.166AlaLys: 6.166 ± 0.683
7.295AlaLeu: 7.295 ± 0.535
2.779AlaMet: 2.779 ± 0.423
4.776AlaAsn: 4.776 ± 0.631
5.297AlaPro: 5.297 ± 0.733
3.734AlaGln: 3.734 ± 0.368
5.645AlaArg: 5.645 ± 0.594
4.168AlaSer: 4.168 ± 0.471
5.905AlaThr: 5.905 ± 0.412
5.905AlaVal: 5.905 ± 0.624
1.737AlaTrp: 1.737 ± 0.261
2.822AlaTyr: 2.822 ± 0.358
0.0AlaXaa: 0.0 ± 0.0
Cys
0.999CysAla: 0.999 ± 0.212
0.087CysCys: 0.087 ± 0.066
0.608CysAsp: 0.608 ± 0.184
0.999CysGlu: 0.999 ± 0.286
0.13CysPhe: 0.13 ± 0.071
0.999CysGly: 0.999 ± 0.238
0.217CysHis: 0.217 ± 0.111
0.434CysIle: 0.434 ± 0.153
0.217CysLys: 0.217 ± 0.092
0.564CysLeu: 0.564 ± 0.187
0.217CysMet: 0.217 ± 0.093
0.347CysAsn: 0.347 ± 0.118
0.304CysPro: 0.304 ± 0.1
0.261CysGln: 0.261 ± 0.113
0.651CysArg: 0.651 ± 0.207
0.391CysSer: 0.391 ± 0.146
0.521CysThr: 0.521 ± 0.166
0.564CysVal: 0.564 ± 0.147
0.087CysTrp: 0.087 ± 0.095
0.174CysTyr: 0.174 ± 0.079
0.0CysXaa: 0.0 ± 0.0
Asp
5.732AspAla: 5.732 ± 0.505
0.651AspCys: 0.651 ± 0.175
5.862AspAsp: 5.862 ± 1.185
5.775AspGlu: 5.775 ± 0.813
2.084AspPhe: 2.084 ± 0.352
5.515AspGly: 5.515 ± 0.718
1.172AspHis: 1.172 ± 0.241
3.126AspIle: 3.126 ± 0.469
3.257AspLys: 3.257 ± 0.482
5.428AspLeu: 5.428 ± 0.531
1.65AspMet: 1.65 ± 0.269
1.867AspAsn: 1.867 ± 0.297
4.255AspPro: 4.255 ± 0.389
1.824AspGln: 1.824 ± 0.29
3.257AspArg: 3.257 ± 0.371
3.04AspSer: 3.04 ± 0.39
3.126AspThr: 3.126 ± 0.383
4.386AspVal: 4.386 ± 0.417
1.607AspTrp: 1.607 ± 0.219
2.562AspTyr: 2.562 ± 0.372
0.0AspXaa: 0.0 ± 0.0
Glu
7.772GluAla: 7.772 ± 0.865
0.304GluCys: 0.304 ± 0.116
4.69GluAsp: 4.69 ± 0.708
4.863GluGlu: 4.863 ± 0.457
2.562GluPhe: 2.562 ± 0.283
4.038GluGly: 4.038 ± 0.456
1.737GluHis: 1.737 ± 0.319
2.909GluIle: 2.909 ± 0.392
3.387GluLys: 3.387 ± 0.493
5.254GluLeu: 5.254 ± 0.559
1.824GluMet: 1.824 ± 0.297
2.605GluAsn: 2.605 ± 0.303
2.301GluPro: 2.301 ± 0.321
2.779GluGln: 2.779 ± 0.379
4.776GluArg: 4.776 ± 0.74
3.343GluSer: 3.343 ± 0.468
3.17GluThr: 3.17 ± 0.354
4.299GluVal: 4.299 ± 0.446
1.259GluTrp: 1.259 ± 0.235
1.954GluTyr: 1.954 ± 0.311
0.0GluXaa: 0.0 ± 0.0
Phe
2.475PheAla: 2.475 ± 0.333
0.217PheCys: 0.217 ± 0.101
2.953PheAsp: 2.953 ± 0.462
2.171PheGlu: 2.171 ± 0.333
0.825PhePhe: 0.825 ± 0.203
3.517PheGly: 3.517 ± 0.395
0.478PheHis: 0.478 ± 0.13
1.563PheIle: 1.563 ± 0.235
1.693PheLys: 1.693 ± 0.398
2.388PheLeu: 2.388 ± 0.265
1.216PheMet: 1.216 ± 0.257
1.172PheAsn: 1.172 ± 0.209
1.607PhePro: 1.607 ± 0.292
1.563PheGln: 1.563 ± 0.349
1.867PheArg: 1.867 ± 0.332
1.389PheSer: 1.389 ± 0.238
1.607PheThr: 1.607 ± 0.309
2.475PheVal: 2.475 ± 0.308
0.391PheTrp: 0.391 ± 0.115
0.868PheTyr: 0.868 ± 0.169
0.0PheXaa: 0.0 ± 0.0
Gly
6.34GlyAla: 6.34 ± 0.795
0.564GlyCys: 0.564 ± 0.147
5.471GlyAsp: 5.471 ± 0.481
4.863GlyGlu: 4.863 ± 0.386
2.779GlyPhe: 2.779 ± 0.331
9.509GlyGly: 9.509 ± 2.006
1.911GlyHis: 1.911 ± 0.274
5.384GlyIle: 5.384 ± 0.576
4.212GlyLys: 4.212 ± 0.822
6.209GlyLeu: 6.209 ± 0.503
2.345GlyMet: 2.345 ± 0.338
3.213GlyAsn: 3.213 ± 0.332
3.734GlyPro: 3.734 ± 0.376
3.43GlyGln: 3.43 ± 0.449
5.167GlyArg: 5.167 ± 0.645
5.254GlySer: 5.254 ± 0.846
4.603GlyThr: 4.603 ± 0.456
5.341GlyVal: 5.341 ± 0.558
1.911GlyTrp: 1.911 ± 0.262
3.43GlyTyr: 3.43 ± 0.504
0.0GlyXaa: 0.0 ± 0.0
His
1.563HisAla: 1.563 ± 0.218
0.261HisCys: 0.261 ± 0.106
0.955HisAsp: 0.955 ± 0.2
1.607HisGlu: 1.607 ± 0.285
0.738HisPhe: 0.738 ± 0.225
2.171HisGly: 2.171 ± 0.435
0.738HisHis: 0.738 ± 0.183
1.129HisIle: 1.129 ± 0.248
1.303HisLys: 1.303 ± 0.266
1.389HisLeu: 1.389 ± 0.267
0.434HisMet: 0.434 ± 0.144
0.608HisAsn: 0.608 ± 0.165
1.997HisPro: 1.997 ± 0.299
0.651HisGln: 0.651 ± 0.193
0.825HisArg: 0.825 ± 0.22
1.172HisSer: 1.172 ± 0.238
0.651HisThr: 0.651 ± 0.179
1.65HisVal: 1.65 ± 0.283
0.347HisTrp: 0.347 ± 0.125
0.521HisTyr: 0.521 ± 0.149
0.0HisXaa: 0.0 ± 0.0
Ile
4.69IleAla: 4.69 ± 0.488
0.261IleCys: 0.261 ± 0.122
4.82IleAsp: 4.82 ± 0.399
3.474IleGlu: 3.474 ± 0.316
1.563IlePhe: 1.563 ± 0.24
2.866IleGly: 2.866 ± 0.303
1.259IleHis: 1.259 ± 0.231
2.605IleIle: 2.605 ± 0.369
2.909IleLys: 2.909 ± 0.345
3.517IleLeu: 3.517 ± 0.433
0.912IleMet: 0.912 ± 0.178
2.084IleAsn: 2.084 ± 0.363
2.605IlePro: 2.605 ± 0.296
1.52IleGln: 1.52 ± 0.271
2.736IleArg: 2.736 ± 0.446
2.258IleSer: 2.258 ± 0.344
2.649IleThr: 2.649 ± 0.394
3.821IleVal: 3.821 ± 0.378
0.955IleTrp: 0.955 ± 0.28
1.042IleTyr: 1.042 ± 0.231
0.0IleXaa: 0.0 ± 0.0
Lys
6.34LysAla: 6.34 ± 0.801
0.478LysCys: 0.478 ± 0.131
3.343LysAsp: 3.343 ± 0.453
2.432LysGlu: 2.432 ± 0.377
1.607LysPhe: 1.607 ± 0.375
3.821LysGly: 3.821 ± 0.59
0.825LysHis: 0.825 ± 0.185
2.041LysIle: 2.041 ± 0.451
3.561LysLys: 3.561 ± 0.844
4.082LysLeu: 4.082 ± 0.608
1.824LysMet: 1.824 ± 0.334
1.693LysAsn: 1.693 ± 0.45
2.953LysPro: 2.953 ± 0.396
2.475LysGln: 2.475 ± 0.43
4.472LysArg: 4.472 ± 0.592
3.126LysSer: 3.126 ± 0.584
2.866LysThr: 2.866 ± 0.375
3.474LysVal: 3.474 ± 0.528
0.868LysTrp: 0.868 ± 0.238
1.867LysTyr: 1.867 ± 0.316
0.0LysXaa: 0.0 ± 0.0
Leu
6.904LeuAla: 6.904 ± 0.47
0.782LeuCys: 0.782 ± 0.219
5.384LeuAsp: 5.384 ± 0.529
4.559LeuGlu: 4.559 ± 0.486
1.65LeuPhe: 1.65 ± 0.291
6.513LeuGly: 6.513 ± 0.754
1.389LeuHis: 1.389 ± 0.308
2.736LeuIle: 2.736 ± 0.401
3.604LeuLys: 3.604 ± 0.525
5.905LeuLeu: 5.905 ± 0.522
1.911LeuMet: 1.911 ± 0.284
3.474LeuAsn: 3.474 ± 0.446
4.125LeuPro: 4.125 ± 0.442
2.605LeuGln: 2.605 ± 0.386
5.167LeuArg: 5.167 ± 0.471
4.168LeuSer: 4.168 ± 0.33
3.908LeuThr: 3.908 ± 0.38
5.08LeuVal: 5.08 ± 0.497
1.259LeuTrp: 1.259 ± 0.193
1.65LeuTyr: 1.65 ± 0.309
0.0LeuXaa: 0.0 ± 0.0
Met
3.083MetAla: 3.083 ± 0.289
0.347MetCys: 0.347 ± 0.124
1.389MetAsp: 1.389 ± 0.231
1.433MetGlu: 1.433 ± 0.243
0.695MetPhe: 0.695 ± 0.153
1.78MetGly: 1.78 ± 0.276
0.217MetHis: 0.217 ± 0.087
1.042MetIle: 1.042 ± 0.194
1.911MetLys: 1.911 ± 0.27
1.954MetLeu: 1.954 ± 0.255
0.217MetMet: 0.217 ± 0.1
0.868MetAsn: 0.868 ± 0.169
1.78MetPro: 1.78 ± 0.266
0.782MetGln: 0.782 ± 0.161
1.867MetArg: 1.867 ± 0.278
2.128MetSer: 2.128 ± 0.259
1.693MetThr: 1.693 ± 0.286
1.086MetVal: 1.086 ± 0.219
0.478MetTrp: 0.478 ± 0.161
0.695MetTyr: 0.695 ± 0.194
0.0MetXaa: 0.0 ± 0.0
Asn
3.734AsnAla: 3.734 ± 0.464
0.087AsnCys: 0.087 ± 0.061
2.041AsnAsp: 2.041 ± 0.314
2.432AsnGlu: 2.432 ± 0.343
1.303AsnPhe: 1.303 ± 0.266
3.951AsnGly: 3.951 ± 0.548
1.216AsnHis: 1.216 ± 0.28
1.997AsnIle: 1.997 ± 0.244
1.78AsnLys: 1.78 ± 0.358
2.649AsnLeu: 2.649 ± 0.422
1.172AsnMet: 1.172 ± 0.189
1.52AsnAsn: 1.52 ± 0.317
3.647AsnPro: 3.647 ± 0.386
0.912AsnGln: 0.912 ± 0.224
1.78AsnArg: 1.78 ± 0.284
1.997AsnSer: 1.997 ± 0.277
2.258AsnThr: 2.258 ± 0.266
2.432AsnVal: 2.432 ± 0.305
0.825AsnTrp: 0.825 ± 0.181
1.303AsnTyr: 1.303 ± 0.217
0.0AsnXaa: 0.0 ± 0.0
Pro
5.254ProAla: 5.254 ± 0.594
0.347ProCys: 0.347 ± 0.145
3.257ProAsp: 3.257 ± 0.329
4.212ProGlu: 4.212 ± 0.613
1.737ProPhe: 1.737 ± 0.283
4.82ProGly: 4.82 ± 0.625
1.303ProHis: 1.303 ± 0.225
2.649ProIle: 2.649 ± 0.341
2.996ProLys: 2.996 ± 0.467
3.474ProLeu: 3.474 ± 0.465
1.563ProMet: 1.563 ± 0.251
2.736ProAsn: 2.736 ± 0.44
3.778ProPro: 3.778 ± 0.486
2.258ProGln: 2.258 ± 0.407
2.475ProArg: 2.475 ± 0.472
2.909ProSer: 2.909 ± 0.369
3.778ProThr: 3.778 ± 0.415
3.821ProVal: 3.821 ± 0.446
1.086ProTrp: 1.086 ± 0.252
1.997ProTyr: 1.997 ± 0.324
0.0ProXaa: 0.0 ± 0.0
Gln
3.908GlnAla: 3.908 ± 0.414
0.391GlnCys: 0.391 ± 0.122
1.65GlnAsp: 1.65 ± 0.31
1.954GlnGlu: 1.954 ± 0.381
1.433GlnPhe: 1.433 ± 0.261
2.692GlnGly: 2.692 ± 0.371
0.868GlnHis: 0.868 ± 0.193
2.041GlnIle: 2.041 ± 0.463
2.171GlnLys: 2.171 ± 0.346
2.866GlnLeu: 2.866 ± 0.443
0.868GlnMet: 0.868 ± 0.179
0.825GlnAsn: 0.825 ± 0.232
1.954GlnPro: 1.954 ± 0.249
1.607GlnGln: 1.607 ± 0.27
2.562GlnArg: 2.562 ± 0.371
2.518GlnSer: 2.518 ± 0.366
2.171GlnThr: 2.171 ± 0.325
2.562GlnVal: 2.562 ± 0.373
0.825GlnTrp: 0.825 ± 0.171
1.086GlnTyr: 1.086 ± 0.198
0.0GlnXaa: 0.0 ± 0.0
Arg
6.079ArgAla: 6.079 ± 0.764
0.782ArgCys: 0.782 ± 0.177
3.343ArgAsp: 3.343 ± 0.438
3.778ArgGlu: 3.778 ± 0.608
2.388ArgPhe: 2.388 ± 0.41
4.082ArgGly: 4.082 ± 0.661
1.216ArgHis: 1.216 ± 0.22
3.778ArgIle: 3.778 ± 0.472
3.865ArgLys: 3.865 ± 0.574
4.733ArgLeu: 4.733 ± 0.522
1.78ArgMet: 1.78 ± 0.31
2.215ArgAsn: 2.215 ± 0.388
3.04ArgPro: 3.04 ± 0.343
2.649ArgGln: 2.649 ± 0.365
5.341ArgArg: 5.341 ± 0.653
2.779ArgSer: 2.779 ± 0.382
3.517ArgThr: 3.517 ± 0.442
4.603ArgVal: 4.603 ± 0.57
1.259ArgTrp: 1.259 ± 0.279
1.52ArgTyr: 1.52 ± 0.331
0.0ArgXaa: 0.0 ± 0.0
Ser
5.601SerAla: 5.601 ± 0.581
0.521SerCys: 0.521 ± 0.154
3.213SerAsp: 3.213 ± 0.323
3.04SerGlu: 3.04 ± 0.422
1.78SerPhe: 1.78 ± 0.351
5.645SerGly: 5.645 ± 0.634
1.129SerHis: 1.129 ± 0.239
2.432SerIle: 2.432 ± 0.29
3.04SerLys: 3.04 ± 0.528
2.996SerLeu: 2.996 ± 0.415
1.433SerMet: 1.433 ± 0.279
2.215SerAsn: 2.215 ± 0.289
2.171SerPro: 2.171 ± 0.327
1.737SerGln: 1.737 ± 0.282
3.343SerArg: 3.343 ± 0.394
2.649SerSer: 2.649 ± 0.485
2.779SerThr: 2.779 ± 0.415
3.213SerVal: 3.213 ± 0.395
1.389SerTrp: 1.389 ± 0.289
1.911SerTyr: 1.911 ± 0.346
0.0SerXaa: 0.0 ± 0.0
Thr
5.124ThrAla: 5.124 ± 0.444
0.521ThrCys: 0.521 ± 0.206
3.343ThrAsp: 3.343 ± 0.358
2.953ThrGlu: 2.953 ± 0.317
2.041ThrPhe: 2.041 ± 0.29
5.905ThrGly: 5.905 ± 0.433
0.999ThrHis: 0.999 ± 0.185
2.605ThrIle: 2.605 ± 0.35
2.301ThrLys: 2.301 ± 0.395
3.821ThrLeu: 3.821 ± 0.367
0.999ThrMet: 0.999 ± 0.212
2.128ThrAsn: 2.128 ± 0.277
4.299ThrPro: 4.299 ± 0.553
1.824ThrGln: 1.824 ± 0.353
3.17ThrArg: 3.17 ± 0.478
2.518ThrSer: 2.518 ± 0.326
3.43ThrThr: 3.43 ± 0.639
3.951ThrVal: 3.951 ± 0.376
1.259ThrTrp: 1.259 ± 0.268
2.041ThrTyr: 2.041 ± 0.276
0.0ThrXaa: 0.0 ± 0.0
Val
6.166ValAla: 6.166 ± 0.508
0.564ValCys: 0.564 ± 0.204
4.038ValAsp: 4.038 ± 0.434
4.82ValGlu: 4.82 ± 0.555
2.041ValPhe: 2.041 ± 0.329
5.688ValGly: 5.688 ± 0.539
1.172ValHis: 1.172 ± 0.224
3.474ValIle: 3.474 ± 0.373
3.343ValLys: 3.343 ± 0.627
4.342ValLeu: 4.342 ± 0.391
1.346ValMet: 1.346 ± 0.226
2.909ValAsn: 2.909 ± 0.295
4.082ValPro: 4.082 ± 0.46
2.301ValGln: 2.301 ± 0.331
4.429ValArg: 4.429 ± 0.422
3.517ValSer: 3.517 ± 0.378
3.734ValThr: 3.734 ± 0.454
6.513ValVal: 6.513 ± 0.601
0.999ValTrp: 0.999 ± 0.229
2.649ValTyr: 2.649 ± 0.258
0.0ValXaa: 0.0 ± 0.0
Trp
1.563TrpAla: 1.563 ± 0.292
0.347TrpCys: 0.347 ± 0.112
1.607TrpAsp: 1.607 ± 0.275
2.041TrpGlu: 2.041 ± 0.368
0.564TrpPhe: 0.564 ± 0.133
1.259TrpGly: 1.259 ± 0.192
0.304TrpHis: 0.304 ± 0.107
0.912TrpIle: 0.912 ± 0.198
0.738TrpLys: 0.738 ± 0.177
2.301TrpLeu: 2.301 ± 0.25
0.478TrpMet: 0.478 ± 0.156
0.651TrpAsn: 0.651 ± 0.177
0.608TrpPro: 0.608 ± 0.2
0.868TrpGln: 0.868 ± 0.253
1.259TrpArg: 1.259 ± 0.258
1.259TrpSer: 1.259 ± 0.28
0.999TrpThr: 0.999 ± 0.175
1.086TrpVal: 1.086 ± 0.23
0.391TrpTrp: 0.391 ± 0.13
0.391TrpTyr: 0.391 ± 0.152
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.432TyrAla: 2.432 ± 0.268
0.391TyrCys: 0.391 ± 0.122
2.562TyrAsp: 2.562 ± 0.345
1.737TyrGlu: 1.737 ± 0.264
1.129TyrPhe: 1.129 ± 0.266
3.865TyrGly: 3.865 ± 0.348
0.999TyrHis: 0.999 ± 0.237
1.389TyrIle: 1.389 ± 0.232
1.693TyrLys: 1.693 ± 0.457
1.607TyrLeu: 1.607 ± 0.296
0.434TyrMet: 0.434 ± 0.146
1.129TyrAsn: 1.129 ± 0.225
1.911TyrPro: 1.911 ± 0.346
1.172TyrGln: 1.172 ± 0.209
1.954TyrArg: 1.954 ± 0.318
1.65TyrSer: 1.65 ± 0.354
1.867TyrThr: 1.867 ± 0.27
1.867TyrVal: 1.867 ± 0.355
0.695TyrTrp: 0.695 ± 0.22
0.782TyrTyr: 0.782 ± 0.212
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 107 proteins (23031 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski