Bacteroides sp. CAG:598

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; environmental samples

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2776 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5C4Q5|R5C4Q5_9BACE Uncharacterized protein OS=Bacteroides sp. CAG:598 OX=1262743 GN=BN727_00959 PE=4 SV=1
MM1 pKa = 7.27YY2 pKa = 10.33KK3 pKa = 9.12RR4 pKa = 11.84TFMQTLAAVAMMLLPAACADD24 pKa = 4.55DD25 pKa = 4.67YY26 pKa = 11.86DD27 pKa = 6.46DD28 pKa = 4.08SALWDD33 pKa = 3.52KK34 pKa = 11.66VNDD37 pKa = 3.69HH38 pKa = 6.3EE39 pKa = 4.58EE40 pKa = 3.74RR41 pKa = 11.84LAALEE46 pKa = 3.96KK47 pKa = 10.09WQEE50 pKa = 3.89QTNQSIAAMQEE61 pKa = 4.14LLNTTDD67 pKa = 3.57MITGVSAVSEE77 pKa = 4.46GGQTVGYY84 pKa = 8.23TITFLHH90 pKa = 6.93SDD92 pKa = 4.91PITIYY97 pKa = 10.82NGAKK101 pKa = 10.15GADD104 pKa = 3.95GEE106 pKa = 4.76DD107 pKa = 3.97GADD110 pKa = 3.73GADD113 pKa = 3.47GQTPQIGLAQAEE125 pKa = 4.35DD126 pKa = 4.52GNWYY130 pKa = 6.21WTLNGEE136 pKa = 4.98LLTDD140 pKa = 3.7AQGNPIRR147 pKa = 11.84ANGLDD152 pKa = 3.77GADD155 pKa = 3.88GADD158 pKa = 3.99GEE160 pKa = 5.04DD161 pKa = 4.26GATGPEE167 pKa = 4.57GPQGNDD173 pKa = 2.98GTSAPTPQILLGSSITSGTIKK194 pKa = 9.88TDD196 pKa = 3.0NGQKK200 pKa = 9.34DD201 pKa = 2.98TDD203 pKa = 3.15AWYY206 pKa = 10.77LSVDD210 pKa = 4.55NGATWYY216 pKa = 10.22RR217 pKa = 11.84VSGKK221 pKa = 10.42DD222 pKa = 3.43GTNGDD227 pKa = 3.38AWFSDD232 pKa = 3.9EE233 pKa = 4.48PKK235 pKa = 10.48KK236 pKa = 10.72EE237 pKa = 3.52GNYY240 pKa = 10.1YY241 pKa = 10.71VFTLTDD247 pKa = 3.06QSTFRR252 pKa = 11.84VAAYY256 pKa = 9.82QPFRR260 pKa = 11.84ILTEE264 pKa = 3.97AEE266 pKa = 3.91KK267 pKa = 11.06DD268 pKa = 3.39GTTTFTNATVTINGATTFYY287 pKa = 10.99LYY289 pKa = 10.19IDD291 pKa = 3.88NDD293 pKa = 3.05IKK295 pKa = 10.92YY296 pKa = 9.88QEE298 pKa = 4.06IVAQISSVDD307 pKa = 3.53AVLTRR312 pKa = 11.84ANVSDD317 pKa = 3.32WTVVKK322 pKa = 9.75GTEE325 pKa = 4.11QNTLTVTPGTQPYY338 pKa = 10.59ALLDD342 pKa = 3.69VSLLLTDD349 pKa = 4.52GSKK352 pKa = 9.23LTASRR357 pKa = 11.84LLEE360 pKa = 4.57DD361 pKa = 4.71PGYY364 pKa = 9.65TDD366 pKa = 5.66DD367 pKa = 4.22GQGNYY372 pKa = 7.72TVTSAEE378 pKa = 3.95GLKK381 pKa = 10.85NIAKK385 pKa = 9.94LVNEE389 pKa = 4.41EE390 pKa = 4.16GKK392 pKa = 9.01TDD394 pKa = 3.57INITLDD400 pKa = 3.19TDD402 pKa = 3.83LTLTGEE408 pKa = 3.99WTPIGTEE415 pKa = 3.94SQPYY419 pKa = 9.24TGTFDD424 pKa = 4.06GGNHH428 pKa = 7.26TITGLKK434 pKa = 9.16IDD436 pKa = 4.07QSGTDD441 pKa = 3.49NVGLIGRR448 pKa = 11.84LGSGGKK454 pKa = 9.09VQDD457 pKa = 3.64VTLTEE462 pKa = 4.22VNVTGGTYY470 pKa = 10.2VGGIAGQTDD479 pKa = 3.94GTVEE483 pKa = 3.93NCSVNGTVTGQNQTGGIVGRR503 pKa = 11.84NFSTISGCSAEE514 pKa = 4.25GTVTGNTNVGGISGLCVPNYY534 pKa = 8.06DD535 pKa = 3.82TGTGSLIGSTIEE547 pKa = 4.47GCHH550 pKa = 4.72STAAVSGISSVGGVVGNLGNGCSLMACYY578 pKa = 9.38STGNVTATITYY589 pKa = 8.56GHH591 pKa = 7.02ANVGGVVGINGQGTVTACYY610 pKa = 9.43HH611 pKa = 5.28ATGEE615 pKa = 4.39ITSSVGDD622 pKa = 4.01RR623 pKa = 11.84IGGIAGCNDD632 pKa = 2.93QGTFTACYY640 pKa = 8.88WEE642 pKa = 4.3NNQEE646 pKa = 3.97QGTGSGSTGGTAKK659 pKa = 10.75VEE661 pKa = 4.49GNVIWKK667 pKa = 8.03TAVDD671 pKa = 3.78AMNAALNKK679 pKa = 10.53SGWTYY684 pKa = 11.48RR685 pKa = 11.84LGGGNLPEE693 pKa = 4.67LVQNQQ698 pKa = 3.27

Molecular weight: 72.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5C8U0|R5C8U0_9BACE Uncharacterized protein OS=Bacteroides sp. CAG:598 OX=1262743 GN=BN727_01105 PE=4 SV=1
MM1 pKa = 7.11FFARR5 pKa = 11.84FVLSLLRR12 pKa = 11.84ITLIRR17 pKa = 11.84QHH19 pKa = 6.19MNNPHH24 pKa = 6.1ILGTEE29 pKa = 4.43RR30 pKa = 11.84IGKK33 pKa = 9.35LLVQYY38 pKa = 8.77SIPAIISMTIVSLYY52 pKa = 10.82NIIDD56 pKa = 4.03SIFIGHH62 pKa = 6.59GVGPMAIAGLAITFPLMNLTAAFCNLVSAGGSTISSIRR100 pKa = 11.84LGQKK104 pKa = 10.49DD105 pKa = 3.62MEE107 pKa = 4.66GVNKK111 pKa = 10.05VLGNTLMLCLINAVVFGGLTFIFLDD136 pKa = 4.49AILRR140 pKa = 11.84FFGASGEE147 pKa = 4.3TLPYY151 pKa = 10.71ARR153 pKa = 11.84DD154 pKa = 3.62FMQVILLGTPITFTMIGLNHH174 pKa = 5.57VMRR177 pKa = 11.84ATGHH181 pKa = 6.35PKK183 pKa = 10.28KK184 pKa = 10.86AMLTSLTTVVCNIILAPIFIFQFGWGIRR212 pKa = 11.84GAALATVISQFIGMLWVLNHH232 pKa = 6.35FLQRR236 pKa = 11.84SNPIRR241 pKa = 11.84LRR243 pKa = 11.84HH244 pKa = 5.28GFQRR248 pKa = 11.84MEE250 pKa = 3.66RR251 pKa = 11.84RR252 pKa = 11.84IVGSIFSIGMSPFLMNVTACAIVIIINNSLQRR284 pKa = 11.84HH285 pKa = 5.66GGDD288 pKa = 3.4MAIGAYY294 pKa = 10.19GIINRR299 pKa = 11.84LLMLYY304 pKa = 10.47VMVVMGLTMGMQPITGYY321 pKa = 10.84NFGARR326 pKa = 11.84RR327 pKa = 11.84LDD329 pKa = 3.25RR330 pKa = 11.84VKK332 pKa = 10.47QVLRR336 pKa = 11.84LGITAGVCITSSGFLICEE354 pKa = 4.72LFPHH358 pKa = 6.83AVASVFTSDD367 pKa = 3.07EE368 pKa = 3.84ALINMAARR376 pKa = 11.84GVRR379 pKa = 11.84ICVAIFPFVGAQIVIGNFFQSIGMAKK405 pKa = 9.95ISIFMSLTRR414 pKa = 11.84QLLYY418 pKa = 10.91LLPCLLVFPQFMGLDD433 pKa = 4.67GIWTSMPVSDD443 pKa = 5.14FFAFVTAVLILLWYY457 pKa = 9.82VRR459 pKa = 11.84RR460 pKa = 11.84KK461 pKa = 10.1HH462 pKa = 6.28LFHH465 pKa = 7.01PQAIQQ470 pKa = 2.88

Molecular weight: 51.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2776

0

2776

985690

29

2799

355.1

39.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.682 ± 0.05

1.329 ± 0.017

5.656 ± 0.036

6.512 ± 0.042

4.386 ± 0.032

7.042 ± 0.047

1.994 ± 0.022

6.2 ± 0.045

5.838 ± 0.036

9.547 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.778 ± 0.02

4.582 ± 0.037

3.85 ± 0.027

3.645 ± 0.029

5.207 ± 0.037

5.701 ± 0.034

5.667 ± 0.036

6.741 ± 0.037

1.311 ± 0.02

4.325 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski