Methanophagales archaeon

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanophagales; unclassified Methanophagales

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1797 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V3JGB0|A0A2V3JGB0_9EURY Uncharacterized protein OS=Methanophagales archaeon OX=2056316 GN=C4B56_04215 PE=4 SV=1
MM1 pKa = 7.25EE2 pKa = 5.67KK3 pKa = 10.82KK4 pKa = 9.31MFKK7 pKa = 10.14PNEE10 pKa = 3.72KK11 pKa = 9.54RR12 pKa = 11.84VCVLRR17 pKa = 11.84NVGIILVGVVVLSLLVCAASTATWTVSEE45 pKa = 4.15EE46 pKa = 3.99GGAGFMRR53 pKa = 11.84IEE55 pKa = 4.47DD56 pKa = 3.8AVVAASSGDD65 pKa = 3.83IIDD68 pKa = 4.83KK69 pKa = 10.95DD70 pKa = 4.3LAQTDD75 pKa = 3.62AKK77 pKa = 11.0LITTLSGEE85 pKa = 4.14AMDD88 pKa = 5.31DD89 pKa = 3.56RR90 pKa = 11.84LHH92 pKa = 5.99ITPSNVNGDD101 pKa = 4.14VNNDD105 pKa = 3.19GYY107 pKa = 11.62DD108 pKa = 3.79DD109 pKa = 4.12VVSGAWGNDD118 pKa = 2.96AGGTNAGRR126 pKa = 11.84VYY128 pKa = 10.2IYY130 pKa = 10.72LGGSTMDD137 pKa = 3.49NSSDD141 pKa = 3.71IIFTGEE147 pKa = 3.68AANDD151 pKa = 3.76EE152 pKa = 4.8LGDD155 pKa = 3.74PAAFAGDD162 pKa = 4.02VNNDD166 pKa = 3.13GYY168 pKa = 11.61DD169 pKa = 3.63DD170 pKa = 4.6VIAGAPYY177 pKa = 10.66NDD179 pKa = 4.68AGGADD184 pKa = 3.58AGRR187 pKa = 11.84AYY189 pKa = 9.85ILFGGNPMDD198 pKa = 5.48NIPDD202 pKa = 3.85VIITGQSPGDD212 pKa = 3.57NLGAWGATYY221 pKa = 10.7AGDD224 pKa = 3.75VNKK227 pKa = 10.9DD228 pKa = 3.14GFDD231 pKa = 3.71DD232 pKa = 4.79VIVSACYY239 pKa = 9.78KK240 pKa = 10.08GPGYY244 pKa = 10.92AYY246 pKa = 9.38IYY248 pKa = 10.55LGGNPMDD255 pKa = 4.02NVSDD259 pKa = 3.86LVLSGEE265 pKa = 4.34STGDD269 pKa = 3.47QFGYY273 pKa = 7.78WCAYY277 pKa = 10.15AGDD280 pKa = 4.19INKK283 pKa = 10.28DD284 pKa = 3.24GYY286 pKa = 11.49DD287 pKa = 3.73DD288 pKa = 4.78VIVGAPRR295 pKa = 11.84YY296 pKa = 10.32NGDD299 pKa = 3.05TGRR302 pKa = 11.84AYY304 pKa = 9.54IYY306 pKa = 10.65FGGKK310 pKa = 9.55PMDD313 pKa = 3.69TTPDD317 pKa = 3.51VILTGSGANHH327 pKa = 6.98KK328 pKa = 9.97FGSVGSAGDD337 pKa = 3.81VNNDD341 pKa = 3.34GYY343 pKa = 10.45PDD345 pKa = 3.86VVVGAHH351 pKa = 6.57RR352 pKa = 11.84SDD354 pKa = 2.96MAYY357 pKa = 9.67IFFGGDD363 pKa = 3.22SMDD366 pKa = 3.45NTADD370 pKa = 3.18VTMSGEE376 pKa = 4.12SSGSFFGYY384 pKa = 9.8GVSSIGDD391 pKa = 3.64VNKK394 pKa = 10.65DD395 pKa = 3.15GYY397 pKa = 11.23SDD399 pKa = 4.01VIIGAPCYY407 pKa = 10.13SDD409 pKa = 3.37SGKK412 pKa = 10.9AYY414 pKa = 9.52IYY416 pKa = 10.7FGGNPMDD423 pKa = 4.54NNPDD427 pKa = 3.0ITITGSSGDD436 pKa = 3.8GFGMTVGFAGDD447 pKa = 3.56VNGDD451 pKa = 3.6GNNDD455 pKa = 3.39FTIGAPQNNINGKK468 pKa = 9.23GRR470 pKa = 11.84TYY472 pKa = 10.37VYY474 pKa = 10.06TVISEE479 pKa = 4.42STFPVHH485 pKa = 6.72NLNTGEE491 pKa = 4.12DD492 pKa = 3.91FEE494 pKa = 5.29TIQEE498 pKa = 4.76AIDD501 pKa = 5.49DD502 pKa = 4.6PDD504 pKa = 3.74TKK506 pKa = 10.69NGHH509 pKa = 5.76TIIVDD514 pKa = 3.15AGTYY518 pKa = 9.05YY519 pKa = 11.05EE520 pKa = 3.99NVVVNKK526 pKa = 10.42SLTLKK531 pKa = 10.53GIGMPVVDD539 pKa = 4.65AGGSGSAITLSADD552 pKa = 4.08GITLEE557 pKa = 5.13GFTATNSGSSYY568 pKa = 9.96PDD570 pKa = 2.99AGIKK574 pKa = 9.99INSNNNAIMDD584 pKa = 3.67NTVCNNNYY592 pKa = 10.06GGIWLSEE599 pKa = 3.93SSNNNLQPTSGIPLL613 pKa = 3.71

Molecular weight: 63.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V3J810|A0A2V3J810_9EURY Radical SAM protein OS=Methanophagales archaeon OX=2056316 GN=C4B56_08085 PE=4 SV=1
MM1 pKa = 7.88AIGKK5 pKa = 6.45NTRR8 pKa = 11.84GRR10 pKa = 11.84KK11 pKa = 9.0LRR13 pKa = 11.84LCKK16 pKa = 10.37ANRR19 pKa = 11.84QNRR22 pKa = 11.84RR23 pKa = 11.84VPAWIMVKK31 pKa = 7.97TNRR34 pKa = 11.84RR35 pKa = 11.84VTTHH39 pKa = 6.29PKK41 pKa = 8.12RR42 pKa = 11.84RR43 pKa = 11.84NWRR46 pKa = 11.84RR47 pKa = 11.84TSLKK51 pKa = 10.22VV52 pKa = 3.08

Molecular weight: 6.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1797

0

1797

535814

34

3891

298.2

33.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.574 ± 0.062

1.311 ± 0.025

5.281 ± 0.049

8.236 ± 0.075

3.842 ± 0.041

7.581 ± 0.056

1.846 ± 0.023

7.891 ± 0.047

6.264 ± 0.058

9.01 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.583 ± 0.029

3.817 ± 0.078

3.94 ± 0.034

1.962 ± 0.028

6.058 ± 0.07

5.796 ± 0.043

5.02 ± 0.066

7.394 ± 0.046

1.07 ± 0.026

3.523 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski