Hwanghaeicola aestuarii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Hwanghaeicola

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4336 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2W7MUH2|A0A2W7MUH2_9RHOB Plasmid stability protein OS=Hwanghaeicola aestuarii OX=568105 GN=LX81_03862 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.41SVIITIGLLAAGVTGAEE19 pKa = 4.06AQQLRR24 pKa = 11.84LMTGPQGGSWYY35 pKa = 9.15PLGGAIQGMASDD47 pKa = 4.61AGIASIQVLPGGGVANVQGVQNGDD71 pKa = 3.29ADD73 pKa = 4.32LGFANSISTVDD84 pKa = 4.33AIAGRR89 pKa = 11.84EE90 pKa = 4.15PFTGAADD97 pKa = 3.78NVCNIATLYY106 pKa = 8.85PQYY109 pKa = 10.44FQIVTTEE116 pKa = 3.96GTGIEE121 pKa = 4.31TPEE124 pKa = 4.12DD125 pKa = 3.52FAGKK129 pKa = 10.01RR130 pKa = 11.84LATQPVGNTAEE141 pKa = 4.17QVTRR145 pKa = 11.84AVLEE149 pKa = 4.36TAGLSYY155 pKa = 11.48DD156 pKa = 3.96DD157 pKa = 5.57LSAVDD162 pKa = 3.85YY163 pKa = 11.08VSYY166 pKa = 11.27SDD168 pKa = 5.2GVSLMQDD175 pKa = 3.09NNSDD179 pKa = 3.29ILTLGTTIPASAIMDD194 pKa = 4.7LANSSDD200 pKa = 3.53VSLVTLEE207 pKa = 3.9QSFIDD212 pKa = 5.25DD213 pKa = 3.28MRR215 pKa = 11.84EE216 pKa = 4.03NINPGYY222 pKa = 8.29TAITIPGGTYY232 pKa = 9.66PGQDD236 pKa = 3.07EE237 pKa = 5.16DD238 pKa = 4.14VTAVGYY244 pKa = 7.65ATHH247 pKa = 6.37VVARR251 pKa = 11.84CDD253 pKa = 3.66LDD255 pKa = 3.89PEE257 pKa = 4.45VVEE260 pKa = 6.36GILTQMWEE268 pKa = 4.47KK269 pKa = 10.38KK270 pKa = 10.61DD271 pKa = 3.64DD272 pKa = 3.55MAAIATVMSDD282 pKa = 3.3LTLEE286 pKa = 4.69SMAQDD291 pKa = 3.44VGVPMHH297 pKa = 7.25DD298 pKa = 3.55GATAFYY304 pKa = 9.14EE305 pKa = 4.27AQGVQQQ311 pKa = 3.96

Molecular weight: 32.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2W7MX97|A0A2W7MX97_9RHOB LuxR family transcriptional regulator OS=Hwanghaeicola aestuarii OX=568105 GN=LX81_03688 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4336

0

4336

1340171

39

4399

309.1

33.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.807 ± 0.048

0.854 ± 0.011

6.191 ± 0.038

6.201 ± 0.037

3.604 ± 0.026

9.018 ± 0.038

1.972 ± 0.019

5.161 ± 0.025

2.295 ± 0.028

10.086 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.609 ± 0.019

2.225 ± 0.018

5.291 ± 0.029

2.682 ± 0.02

7.662 ± 0.044

5.151 ± 0.023

5.431 ± 0.027

7.272 ± 0.029

1.388 ± 0.016

2.1 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski