Amino acid dipepetide frequency for Microbacterium sp.

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.719AlaAla: 19.719 ± 0.221	0.756AlaCys: 0.756 ± 0.033	8.692AlaAsp: 8.692 ± 0.106	7.684AlaGlu: 7.684 ± 0.1	3.813AlaPhe: 3.813 ± 0.073	11.992AlaGly: 11.992 ± 0.134	2.744AlaHis: 2.744 ± 0.057	5.301AlaIle: 5.301 ± 0.09	2.582AlaLys: 2.582 ± 0.071	13.939AlaLeu: 13.939 ± 0.138	2.598AlaMet: 2.598 ± 0.055	2.192AlaAsn: 2.192 ± 0.048	6.449AlaPro: 6.449 ± 0.095	3.798AlaGln: 3.798 ± 0.067	9.641AlaArg: 9.641 ± 0.114	6.97AlaSer: 6.97 ± 0.086	7.431AlaThr: 7.431 ± 0.1	11.921AlaVal: 11.921 ± 0.143	2.049AlaTrp: 2.049 ± 0.039	2.516AlaTyr: 2.516 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	0.736CysAla: 0.736 ± 0.032	0.06CysCys: 0.06 ± 0.008	0.394CysAsp: 0.394 ± 0.019	0.279CysGlu: 0.279 ± 0.018	0.161CysPhe: 0.161 ± 0.015	0.648CysGly: 0.648 ± 0.023	0.145CysHis: 0.145 ± 0.012	0.199CysIle: 0.199 ± 0.014	0.071CysLys: 0.071 ± 0.009	0.477CysLeu: 0.477 ± 0.021	0.089CysMet: 0.089 ± 0.01	0.107CysAsn: 0.107 ± 0.011	0.31CysPro: 0.31 ± 0.018	0.134CysGln: 0.134 ± 0.011	0.397CysArg: 0.397 ± 0.021	0.321CysSer: 0.321 ± 0.018	0.391CysThr: 0.391 ± 0.021	0.442CysVal: 0.442 ± 0.023	0.085CysTrp: 0.085 ± 0.008	0.117CysTyr: 0.117 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	9.236AspAla: 9.236 ± 0.116	0.269AspCys: 0.269 ± 0.016	4.902AspAsp: 4.902 ± 0.084	4.305AspGlu: 4.305 ± 0.07	1.757AspPhe: 1.757 ± 0.047	6.554AspGly: 6.554 ± 0.095	1.329AspHis: 1.329 ± 0.042	2.469AspIle: 2.469 ± 0.049	1.036AspLys: 1.036 ± 0.036	6.813AspLeu: 6.813 ± 0.094	0.902AspMet: 0.902 ± 0.032	1.025AspAsn: 1.025 ± 0.037	4.359AspPro: 4.359 ± 0.078	1.556AspGln: 1.556 ± 0.038	4.751AspArg: 4.751 ± 0.074	2.596AspSer: 2.596 ± 0.051	3.06AspThr: 3.06 ± 0.06	6.027AspVal: 6.027 ± 0.093	0.969AspTrp: 0.969 ± 0.03	1.329AspTyr: 1.329 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	6.837GluAla: 6.837 ± 0.104	0.263GluCys: 0.263 ± 0.016	2.594GluAsp: 2.594 ± 0.058	2.878GluGlu: 2.878 ± 0.057	1.651GluPhe: 1.651 ± 0.038	4.145GluGly: 4.145 ± 0.065	1.592GluHis: 1.592 ± 0.044	2.624GluIle: 2.624 ± 0.059	1.378GluLys: 1.378 ± 0.04	6.258GluLeu: 6.258 ± 0.094	0.932GluMet: 0.932 ± 0.032	1.163GluAsn: 1.163 ± 0.033	2.923GluPro: 2.923 ± 0.06	2.176GluGln: 2.176 ± 0.052	5.046GluArg: 5.046 ± 0.086	2.683GluSer: 2.683 ± 0.049	2.949GluThr: 2.949 ± 0.064	4.698GluVal: 4.698 ± 0.077	0.868GluTrp: 0.868 ± 0.035	1.137GluTyr: 1.137 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	4.229PheAla: 4.229 ± 0.072	0.169PheCys: 0.169 ± 0.013	2.368PheAsp: 2.368 ± 0.052	1.653PheGlu: 1.653 ± 0.039	1.045PhePhe: 1.045 ± 0.036	3.34PheGly: 3.34 ± 0.063	0.617PheHis: 0.617 ± 0.027	1.063PheIle: 1.063 ± 0.046	0.436PheLys: 0.436 ± 0.021	2.806PheLeu: 2.806 ± 0.063	0.433PheMet: 0.433 ± 0.021	0.642PheAsn: 0.642 ± 0.025	1.389PhePro: 1.389 ± 0.037	0.754PheGln: 0.754 ± 0.027	1.865PheArg: 1.865 ± 0.042	1.711PheSer: 1.711 ± 0.044	2.131PheThr: 2.131 ± 0.052	2.837PheVal: 2.837 ± 0.066	0.461PheTrp: 0.461 ± 0.024	0.672PheTyr: 0.672 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	10.559GlyAla: 10.559 ± 0.116	0.645GlyCys: 0.645 ± 0.026	5.642GlyAsp: 5.642 ± 0.075	4.983GlyGlu: 4.983 ± 0.078	3.154GlyPhe: 3.154 ± 0.061	7.565GlyGly: 7.565 ± 0.114	1.972GlyHis: 1.972 ± 0.052	4.457GlyIle: 4.457 ± 0.074	2.136GlyLys: 2.136 ± 0.044	8.717GlyLeu: 8.717 ± 0.105	1.953GlyMet: 1.953 ± 0.049	1.577GlyAsn: 1.577 ± 0.045	3.935GlyPro: 3.935 ± 0.065	2.39GlyGln: 2.39 ± 0.06	6.613GlyArg: 6.613 ± 0.091	5.261GlySer: 5.261 ± 0.084	5.721GlyThr: 5.721 ± 0.089	8.025GlyVal: 8.025 ± 0.087	1.666GlyTrp: 1.666 ± 0.039	2.318GlyTyr: 2.318 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	2.656HisAla: 2.656 ± 0.06	0.128HisCys: 0.128 ± 0.013	1.494HisAsp: 1.494 ± 0.043	1.157HisGlu: 1.157 ± 0.039	0.602HisPhe: 0.602 ± 0.023	2.181HisGly: 2.181 ± 0.051	0.703HisHis: 0.703 ± 0.029	0.74HisIle: 0.74 ± 0.028	0.299HisLys: 0.299 ± 0.018	2.229HisLeu: 2.229 ± 0.05	0.341HisMet: 0.341 ± 0.019	0.358HisAsn: 0.358 ± 0.021	1.581HisPro: 1.581 ± 0.04	0.519HisGln: 0.519 ± 0.024	1.83HisArg: 1.83 ± 0.044	0.912HisSer: 0.912 ± 0.03	1.127HisThr: 1.127 ± 0.038	1.917HisVal: 1.917 ± 0.046	0.3HisTrp: 0.3 ± 0.021	0.445HisTyr: 0.445 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.496IleAla: 6.496 ± 0.081	0.255IleCys: 0.255 ± 0.017	3.479IleAsp: 3.479 ± 0.059	2.664IleGlu: 2.664 ± 0.053	1.087IlePhe: 1.087 ± 0.039	4.421IleGly: 4.421 ± 0.071	0.744IleHis: 0.744 ± 0.028	1.65IleIle: 1.65 ± 0.045	0.791IleLys: 0.791 ± 0.032	3.39IleLeu: 3.39 ± 0.061	0.615IleMet: 0.615 ± 0.027	0.839IleAsn: 0.839 ± 0.032	2.181IlePro: 2.181 ± 0.051	0.888IleGln: 0.888 ± 0.028	2.451IleArg: 2.451 ± 0.055	2.219IleSer: 2.219 ± 0.052	2.724IleThr: 2.724 ± 0.049	4.412IleVal: 4.412 ± 0.07	0.479IleTrp: 0.479 ± 0.025	0.731IleTyr: 0.731 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	2.503LysAla: 2.503 ± 0.062	0.075LysCys: 0.075 ± 0.009	1.086LysAsp: 1.086 ± 0.033	0.949LysGlu: 0.949 ± 0.04	0.467LysPhe: 0.467 ± 0.024	1.592LysGly: 1.592 ± 0.04	0.448LysHis: 0.448 ± 0.021	0.926LysIle: 0.926 ± 0.033	0.809LysLys: 0.809 ± 0.038	1.867LysLeu: 1.867 ± 0.051	0.354LysMet: 0.354 ± 0.019	0.506LysAsn: 0.506 ± 0.024	1.191LysPro: 1.191 ± 0.04	0.628LysGln: 0.628 ± 0.027	1.429LysArg: 1.429 ± 0.043	1.122LysSer: 1.122 ± 0.033	1.342LysThr: 1.342 ± 0.045	1.872LysVal: 1.872 ± 0.046	0.212LysTrp: 0.212 ± 0.014	0.438LysTyr: 0.438 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	14.306LeuAla: 14.306 ± 0.156	0.558LeuCys: 0.558 ± 0.023	7.048LeuAsp: 7.048 ± 0.086	4.901LeuGlu: 4.901 ± 0.071	2.8LeuPhe: 2.8 ± 0.057	8.957LeuGly: 8.957 ± 0.113	2.046LeuHis: 2.046 ± 0.048	3.925LeuIle: 3.925 ± 0.069	1.792LeuLys: 1.792 ± 0.046	9.973LeuLeu: 9.973 ± 0.136	1.607LeuMet: 1.607 ± 0.042	1.75LeuAsn: 1.75 ± 0.042	5.518LeuPro: 5.518 ± 0.079	2.519LeuGln: 2.519 ± 0.048	7.818LeuArg: 7.818 ± 0.102	5.697LeuSer: 5.697 ± 0.084	6.602LeuThr: 6.602 ± 0.081	9.512LeuVal: 9.512 ± 0.11	1.295LeuTrp: 1.295 ± 0.039	1.747LeuTyr: 1.747 ± 0.043	0.0LeuXaa: 0.0 ± 0.0
Met	2.093MetAla: 2.093 ± 0.042	0.112MetCys: 0.112 ± 0.012	0.777MetAsp: 0.777 ± 0.027	0.651MetGlu: 0.651 ± 0.026	0.528MetPhe: 0.528 ± 0.023	1.318MetGly: 1.318 ± 0.042	0.314MetHis: 0.314 ± 0.02	0.82MetIle: 0.82 ± 0.032	0.42MetLys: 0.42 ± 0.024	1.918MetLeu: 1.918 ± 0.044	0.361MetMet: 0.361 ± 0.021	0.471MetAsn: 0.471 ± 0.02	1.149MetPro: 1.149 ± 0.036	0.454MetGln: 0.454 ± 0.023	1.445MetArg: 1.445 ± 0.032	1.452MetSer: 1.452 ± 0.036	1.704MetThr: 1.704 ± 0.042	1.415MetVal: 1.415 ± 0.035	0.233MetTrp: 0.233 ± 0.016	0.323MetTyr: 0.323 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.326AsnAla: 2.326 ± 0.055	0.112AsnCys: 0.112 ± 0.01	1.148AsnAsp: 1.148 ± 0.037	0.904AsnGlu: 0.904 ± 0.034	0.59AsnPhe: 0.59 ± 0.028	1.822AsnGly: 1.822 ± 0.049	0.338AsnHis: 0.338 ± 0.021	0.756AsnIle: 0.756 ± 0.027	0.391AsnLys: 0.391 ± 0.022	1.903AsnLeu: 1.903 ± 0.045	0.318AsnMet: 0.318 ± 0.019	0.461AsnAsn: 0.461 ± 0.025	1.509AsnPro: 1.509 ± 0.044	0.561AsnGln: 0.561 ± 0.024	1.195AsnArg: 1.195 ± 0.033	0.89AsnSer: 0.89 ± 0.031	1.124AsnThr: 1.124 ± 0.039	1.631AsnVal: 1.631 ± 0.04	0.302AsnTrp: 0.302 ± 0.017	0.418AsnTyr: 0.418 ± 0.024	0.0AsnXaa: 0.0 ± 0.0
Pro	7.054ProAla: 7.054 ± 0.096	0.227ProCys: 0.227 ± 0.017	4.093ProAsp: 4.093 ± 0.071	3.638ProGlu: 3.638 ± 0.057	1.646ProPhe: 1.646 ± 0.042	5.078ProGly: 5.078 ± 0.08	1.274ProHis: 1.274 ± 0.037	1.957ProIle: 1.957 ± 0.048	0.985ProLys: 0.985 ± 0.031	4.918ProLeu: 4.918 ± 0.066	0.881ProMet: 0.881 ± 0.029	0.929ProAsn: 0.929 ± 0.033	2.354ProPro: 2.354 ± 0.059	1.511ProGln: 1.511 ± 0.042	3.641ProArg: 3.641 ± 0.068	3.222ProSer: 3.222 ± 0.057	3.343ProThr: 3.343 ± 0.069	5.142ProVal: 5.142 ± 0.063	0.898ProTrp: 0.898 ± 0.034	1.106ProTyr: 1.106 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	3.372GlnAla: 3.372 ± 0.062	0.128GlnCys: 0.128 ± 0.011	1.263GlnAsp: 1.263 ± 0.035	1.238GlnGlu: 1.238 ± 0.034	0.89GlnPhe: 0.89 ± 0.032	1.992GlnGly: 1.992 ± 0.048	0.685GlnHis: 0.685 ± 0.025	1.373GlnIle: 1.373 ± 0.036	0.665GlnLys: 0.665 ± 0.026	3.048GlnLeu: 3.048 ± 0.057	0.505GlnMet: 0.505 ± 0.023	0.583GlnAsn: 0.583 ± 0.028	1.477GlnPro: 1.477 ± 0.043	1.12GlnGln: 1.12 ± 0.037	2.472GlnArg: 2.472 ± 0.056	1.421GlnSer: 1.421 ± 0.036	1.54GlnThr: 1.54 ± 0.038	2.483GlnVal: 2.483 ± 0.053	0.502GlnTrp: 0.502 ± 0.024	0.58GlnTyr: 0.58 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	9.116ArgAla: 9.116 ± 0.113	0.378ArgCys: 0.378 ± 0.02	4.769ArgAsp: 4.769 ± 0.075	4.351ArgGlu: 4.351 ± 0.07	2.413ArgPhe: 2.413 ± 0.054	5.687ArgGly: 5.687 ± 0.077	1.702ArgHis: 1.702 ± 0.038	3.665ArgIle: 3.665 ± 0.07	1.403ArgLys: 1.403 ± 0.041	7.501ArgLeu: 7.501 ± 0.092	1.636ArgMet: 1.636 ± 0.039	1.286ArgAsn: 1.286 ± 0.034	3.898ArgPro: 3.898 ± 0.07	2.057ArgGln: 2.057 ± 0.048	6.794ArgArg: 6.794 ± 0.108	4.221ArgSer: 4.221 ± 0.069	4.688ArgThr: 4.688 ± 0.073	6.39ArgVal: 6.39 ± 0.095	1.3ArgTrp: 1.3 ± 0.038	1.589ArgTyr: 1.589 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	6.977SerAla: 6.977 ± 0.093	0.313SerCys: 0.313 ± 0.021	3.261SerAsp: 3.261 ± 0.065	2.615SerGlu: 2.615 ± 0.056	1.877SerPhe: 1.877 ± 0.05	5.584SerGly: 5.584 ± 0.078	1.107SerHis: 1.107 ± 0.036	2.32SerIle: 2.32 ± 0.046	1.082SerLys: 1.082 ± 0.037	5.299SerLeu: 5.299 ± 0.076	1.122SerMet: 1.122 ± 0.035	0.955SerAsn: 0.955 ± 0.031	3.101SerPro: 3.101 ± 0.059	1.444SerGln: 1.444 ± 0.046	3.916SerArg: 3.916 ± 0.066	3.391SerSer: 3.391 ± 0.066	3.561SerThr: 3.561 ± 0.063	4.748SerVal: 4.748 ± 0.078	0.912SerTrp: 0.912 ± 0.03	1.249SerTyr: 1.249 ± 0.035	0.0SerXaa: 0.0 ± 0.0
Thr	8.179ThrAla: 8.179 ± 0.103	0.331ThrCys: 0.331 ± 0.021	3.727ThrAsp: 3.727 ± 0.067	2.928ThrGlu: 2.928 ± 0.05	1.998ThrPhe: 1.998 ± 0.045	5.928ThrGly: 5.928 ± 0.079	1.232ThrHis: 1.232 ± 0.036	2.68ThrIle: 2.68 ± 0.055	1.14ThrLys: 1.14 ± 0.035	5.951ThrLeu: 5.951 ± 0.079	1.063ThrMet: 1.063 ± 0.034	1.185ThrAsn: 1.185 ± 0.037	3.942ThrPro: 3.942 ± 0.072	1.486ThrGln: 1.486 ± 0.042	3.976ThrArg: 3.976 ± 0.07	3.5ThrSer: 3.5 ± 0.068	3.965ThrThr: 3.965 ± 0.065	6.095ThrVal: 6.095 ± 0.091	1.004ThrTrp: 1.004 ± 0.034	1.407ThrTyr: 1.407 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	12.184ValAla: 12.184 ± 0.121	0.536ValCys: 0.536 ± 0.022	6.141ValAsp: 6.141 ± 0.081	5.115ValGlu: 5.115 ± 0.076	2.856ValPhe: 2.856 ± 0.064	7.385ValGly: 7.385 ± 0.096	1.844ValHis: 1.844 ± 0.045	4.228ValIle: 4.228 ± 0.066	1.695ValLys: 1.695 ± 0.046	9.51ValLeu: 9.51 ± 0.116	1.6ValMet: 1.6 ± 0.041	1.78ValAsn: 1.78 ± 0.05	4.84ValPro: 4.84 ± 0.061	2.196ValGln: 2.196 ± 0.047	6.615ValArg: 6.615 ± 0.099	5.1ValSer: 5.1 ± 0.072	6.014ValThr: 6.014 ± 0.078	9.517ValVal: 9.517 ± 0.116	1.222ValTrp: 1.222 ± 0.032	1.7ValTyr: 1.7 ± 0.044	0.0ValXaa: 0.0 ± 0.0
Trp	1.588TrpAla: 1.588 ± 0.042	0.112TrpCys: 0.112 ± 0.012	0.867TrpAsp: 0.867 ± 0.029	0.697TrpGlu: 0.697 ± 0.028	0.559TrpPhe: 0.559 ± 0.024	1.132TrpGly: 1.132 ± 0.037	0.354TrpHis: 0.354 ± 0.023	0.702TrpIle: 0.702 ± 0.032	0.33TrpLys: 0.33 ± 0.017	1.701TrpLeu: 1.701 ± 0.049	0.378TrpMet: 0.378 ± 0.019	0.449TrpAsn: 0.449 ± 0.022	0.729TrpPro: 0.729 ± 0.027	0.579TrpGln: 0.579 ± 0.028	1.267TrpArg: 1.267 ± 0.039	1.031TrpSer: 1.031 ± 0.033	1.054TrpThr: 1.054 ± 0.034	1.24TrpVal: 1.24 ± 0.04	0.391TrpTrp: 0.391 ± 0.019	0.33TrpTyr: 0.33 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.62TyrAla: 2.62 ± 0.052	0.138TyrCys: 0.138 ± 0.013	1.499TyrAsp: 1.499 ± 0.046	1.206TyrGlu: 1.206 ± 0.035	0.727TyrPhe: 0.727 ± 0.031	1.967TyrGly: 1.967 ± 0.051	0.327TyrHis: 0.327 ± 0.016	0.66TyrIle: 0.66 ± 0.029	0.373TyrLys: 0.373 ± 0.02	2.153TyrLeu: 2.153 ± 0.049	0.273TyrMet: 0.273 ± 0.017	0.443TyrAsn: 0.443 ± 0.021	1.062TyrPro: 1.062 ± 0.04	0.565TyrGln: 0.565 ± 0.026	1.68TyrArg: 1.68 ± 0.042	1.114TyrSer: 1.114 ± 0.033	1.246TyrThr: 1.246 ± 0.036	1.754TyrVal: 1.754 ± 0.044	0.344TyrTrp: 0.344 ± 0.019	0.476TyrTyr: 0.476 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4036 proteins (970863 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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