Alicyclobacillus acidocaldarius subsp. acidocaldarius (strain ATCC 27009 / DSM 446 / BCRC 14685 / JCM 5260 / KCTC 1825 / NBRC 15652 / NCIMB 11725 / NRRL B-14509 / 104-IA) (Bacillus acidocaldarius)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Alicyclobacillaceae; Alicyclobacillus; Alicyclobacillus acidocaldarius; Alicyclobacillus acidocaldarius subsp. acidocaldarius

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3059 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C8WT77|C8WT77_ALIAD Uncharacterized protein OS=Alicyclobacillus acidocaldarius subsp. acidocaldarius (strain ATCC 27009 / DSM 446 / BCRC 14685 / JCM 5260 / KCTC 1825 / NBRC 15652 / NCIMB 11725 / NRRL B-14509 / 104-IA) OX=521098 GN=Aaci_2587 PE=4 SV=1
MM1 pKa = 8.12RR2 pKa = 11.84ITPWMEE8 pKa = 3.59QQQFLYY14 pKa = 11.04NMEE17 pKa = 4.96NINTAMQNVQEE28 pKa = 4.28EE29 pKa = 4.85LSTGKK34 pKa = 9.99VLNLPQDD41 pKa = 3.7DD42 pKa = 4.06PVAVSEE48 pKa = 5.35DD49 pKa = 3.42MDD51 pKa = 4.12LSAEE55 pKa = 4.17VSQVSASLSTISTGLSWMNATQTAIQGIISQLQQIQSDD93 pKa = 3.33IVAALNTPNQSQGGLAGYY111 pKa = 9.35AATVSQLIQGVYY123 pKa = 9.99QIADD127 pKa = 3.18QMVGNRR133 pKa = 11.84YY134 pKa = 9.66LFGGQADD141 pKa = 3.83NTAPTTYY148 pKa = 10.64LKK150 pKa = 9.96TSPTDD155 pKa = 3.46TPQPIPPGAGPSSGSRR171 pKa = 11.84SYY173 pKa = 11.87DD174 pKa = 3.34PFDD177 pKa = 5.41DD178 pKa = 4.4SQASPPNINYY188 pKa = 10.03QIADD192 pKa = 3.84GVSVRR197 pKa = 11.84VTVTAYY203 pKa = 10.64DD204 pKa = 3.84LFLVQPSSTAPNLQDD219 pKa = 3.43TLNNIQTDD227 pKa = 3.86IYY229 pKa = 10.67SGNSGNLKK237 pKa = 9.98QDD239 pKa = 3.57LSDD242 pKa = 3.68LQVNLNQVINLNAALGARR260 pKa = 11.84IQRR263 pKa = 11.84MTAAQNQLTQYY274 pKa = 8.03QTNLTDD280 pKa = 3.67LKK282 pKa = 11.11GGIEE286 pKa = 4.33GADD289 pKa = 3.55MARR292 pKa = 11.84VLTQFSTDD300 pKa = 3.37QVIYY304 pKa = 10.24EE305 pKa = 4.53AALQMGAQILLPSLVNYY322 pKa = 10.09LPSS325 pKa = 3.43

Molecular weight: 34.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C8WVG3|C8WVG3_ALIAD NUDIX hydrolase OS=Alicyclobacillus acidocaldarius subsp. acidocaldarius (strain ATCC 27009 / DSM 446 / BCRC 14685 / JCM 5260 / KCTC 1825 / NBRC 15652 / NCIMB 11725 / NRRL B-14509 / 104-IA) OX=521098 GN=Aaci_1047 PE=3 SV=1
MM1 pKa = 7.52KK2 pKa = 8.55PTYY5 pKa = 9.7QPNVRR10 pKa = 11.84KK11 pKa = 9.84RR12 pKa = 11.84KK13 pKa = 8.89KK14 pKa = 8.99NHH16 pKa = 5.0GFRR19 pKa = 11.84KK20 pKa = 10.05RR21 pKa = 11.84MATKK25 pKa = 9.89GGRR28 pKa = 11.84RR29 pKa = 11.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3059

0

3059

925247

31

2716

302.5

33.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.02 ± 0.063

0.893 ± 0.013

5.162 ± 0.033

6.477 ± 0.051

3.581 ± 0.032

7.778 ± 0.043

2.395 ± 0.019

4.948 ± 0.035

3.075 ± 0.034

10.002 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.605 ± 0.021

2.481 ± 0.035

5.035 ± 0.033

3.534 ± 0.04

7.667 ± 0.062

5.294 ± 0.033

4.896 ± 0.035

8.678 ± 0.043

1.627 ± 0.024

2.852 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski