Paracoccus sphaerophysae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Paracoccus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2819 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A099FFX6|A0A099FFX6_9RHOB Membrane protein OS=Paracoccus sphaerophysae OX=690417 GN=IC63_01415 PE=4 SV=1
MM1 pKa = 7.64ISGPDD6 pKa = 3.43TLIPADD12 pKa = 3.88PRR14 pKa = 11.84RR15 pKa = 11.84SLQEE19 pKa = 3.97KK20 pKa = 9.8TIMAYY25 pKa = 10.07DD26 pKa = 3.64GRR28 pKa = 11.84IEE30 pKa = 4.3TGTSRR35 pKa = 11.84TGVISYY41 pKa = 10.28NGEE44 pKa = 3.54YY45 pKa = 10.66DD46 pKa = 3.51SFNTTFIAGLTYY58 pKa = 10.69SVAAKK63 pKa = 9.88GASSGSGSLADD74 pKa = 4.5PNIALYY80 pKa = 10.68SGNTRR85 pKa = 11.84LLFNDD90 pKa = 4.64DD91 pKa = 3.82VNPPNDD97 pKa = 3.74STPGTNRR104 pKa = 11.84DD105 pKa = 3.28GQLTFTIGAGGTGVYY120 pKa = 10.21SLVVGEE126 pKa = 4.52QGNNATGSYY135 pKa = 8.92TLTVSAGYY143 pKa = 10.55ASNAADD149 pKa = 3.5NVTGTAYY156 pKa = 10.91SDD158 pKa = 5.02AIHH161 pKa = 6.76GMAGNDD167 pKa = 3.79LLYY170 pKa = 11.35GMGGNDD176 pKa = 3.46NLIGGTGNDD185 pKa = 3.6TLLGGDD191 pKa = 4.73LGDD194 pKa = 3.88VLQGQAGDD202 pKa = 3.68DD203 pKa = 3.97FLRR206 pKa = 11.84GQAGNDD212 pKa = 3.03ILYY215 pKa = 10.0GATGADD221 pKa = 3.71DD222 pKa = 5.48LYY224 pKa = 11.27GGTGADD230 pKa = 3.16TFRR233 pKa = 11.84FLSHH237 pKa = 6.82AEE239 pKa = 3.97SNSRR243 pKa = 11.84YY244 pKa = 9.61GVDD247 pKa = 3.98VIAGADD253 pKa = 3.49GAIAFEE259 pKa = 4.47GVGVVGGDD267 pKa = 3.51VLDD270 pKa = 4.37LRR272 pKa = 11.84GMDD275 pKa = 3.97ANLLVAGNQDD285 pKa = 3.83FVFSTSRR292 pKa = 11.84AAGTVALSEE301 pKa = 4.46SGGNTVLSGHH311 pKa = 5.77VNNDD315 pKa = 2.49GVADD319 pKa = 4.09FTIVIADD326 pKa = 4.03GSISAYY332 pKa = 10.24DD333 pKa = 3.37YY334 pKa = 11.2SANEE338 pKa = 3.84FLLL341 pKa = 4.51

Molecular weight: 34.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A099FCU1|A0A099FCU1_9RHOB Enoyl-CoA hydratase OS=Paracoccus sphaerophysae OX=690417 GN=IC63_06620 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.36GGRR28 pKa = 11.84KK29 pKa = 8.97VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.71GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 4.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2819

0

2819

829420

26

1857

294.2

31.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.106 ± 0.07

0.793 ± 0.014

6.131 ± 0.045

5.316 ± 0.043

3.333 ± 0.028

9.208 ± 0.047

1.998 ± 0.025

4.795 ± 0.032

2.464 ± 0.041

10.097 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.7 ± 0.026

2.193 ± 0.023

5.539 ± 0.037

3.038 ± 0.026

7.688 ± 0.048

4.489 ± 0.029

5.316 ± 0.032

7.383 ± 0.039

1.476 ± 0.02

1.937 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski