Influenza A virus (strain A/little yellow-shouldered bat/Guatemala/153/2009(H17N10))
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H6QM74|H6QM74_I09A8 Nucleoprotein OS=Influenza A virus (strain A/little yellow-shouldered bat/Guatemala/153/2009(H17N10)) OX=1129345 GN=NP PE=3 SV=1
MM1 pKa = 7.41 EE2 pKa = 6.43 LIVLLILLNPYY13 pKa = 9.2 TFVLGDD19 pKa = 4.44 RR20 pKa = 11.84 ICIGYY25 pKa = 8.49 QANQNNQTVNTLLEE39 pKa = 4.12 QNVPVTGAQEE49 pKa = 3.87 ILEE52 pKa = 4.41 TNHH55 pKa = 6.33 NGKK58 pKa = 9.29 LCSLNGVPPLDD69 pKa = 4.05 LQSCTLAGWLLGNPNCDD86 pKa = 3.14 SLLEE90 pKa = 4.24 AEE92 pKa = 4.23 EE93 pKa = 3.8 WSYY96 pKa = 11.85 IKK98 pKa = 10.49 INEE101 pKa = 4.26 SAPDD105 pKa = 3.87 DD106 pKa = 3.83 LCFPGNFEE114 pKa = 4.39 NLQDD118 pKa = 4.78 LLLEE122 pKa = 4.12 MSGVQNFTKK131 pKa = 10.57 VKK133 pKa = 10.12 LFNPQSMTGVTTNNVDD149 pKa = 3.33 QTCPFEE155 pKa = 4.68 GKK157 pKa = 9.62 PSFYY161 pKa = 10.79 RR162 pKa = 11.84 NLNWIQGNSGLPFNIEE178 pKa = 3.72 IKK180 pKa = 10.87 NPTSNPLLLLWGIHH194 pKa = 4.61 NTKK197 pKa = 10.49 DD198 pKa = 3.03 AAQQRR203 pKa = 11.84 NLYY206 pKa = 9.54 GNDD209 pKa = 3.05 YY210 pKa = 10.62 SYY212 pKa = 11.22 TIFNFGEE219 pKa = 4.01 KK220 pKa = 9.83 SEE222 pKa = 4.13 EE223 pKa = 3.67 FRR225 pKa = 11.84 PEE227 pKa = 3.02 IGQRR231 pKa = 11.84 DD232 pKa = 4.06 EE233 pKa = 4.83 VKK235 pKa = 10.53 AHH237 pKa = 6.03 QDD239 pKa = 3.18 RR240 pKa = 11.84 IDD242 pKa = 4.02 YY243 pKa = 10.38 YY244 pKa = 10.08 WGSLPAQSTLRR255 pKa = 11.84 IEE257 pKa = 4.34 STGNLIAPEE266 pKa = 3.71 YY267 pKa = 9.91 GFYY270 pKa = 10.8 YY271 pKa = 9.98 KK272 pKa = 10.4 RR273 pKa = 11.84 KK274 pKa = 7.93 EE275 pKa = 4.23 GKK277 pKa = 10.15 GGLMKK282 pKa = 10.68 SKK284 pKa = 10.71 LPISDD289 pKa = 5.04 CSTKK293 pKa = 10.63 CQTPLGALNSTLPFQNVHH311 pKa = 5.06 QQTIGNCPKK320 pKa = 10.16 YY321 pKa = 11.03 VKK323 pKa = 9.74 ATSLMLATGLRR334 pKa = 11.84 NNPQMEE340 pKa = 4.17 GRR342 pKa = 11.84 GLFGAIAGFIEE353 pKa = 5.0 GGWQGMIDD361 pKa = 3.34 GWYY364 pKa = 10.11 GYY366 pKa = 9.32 HH367 pKa = 7.04 HH368 pKa = 7.2 EE369 pKa = 4.18 NQEE372 pKa = 4.07 GSGYY376 pKa = 10.86 AADD379 pKa = 5.01 KK380 pKa = 10.51 EE381 pKa = 4.49 ATQKK385 pKa = 11.19 AVDD388 pKa = 4.46 AITNKK393 pKa = 9.81 VNSIIDD399 pKa = 3.38 KK400 pKa = 9.93 MNSQFEE406 pKa = 4.63 SNIKK410 pKa = 9.7 EE411 pKa = 4.08 FNRR414 pKa = 11.84 LEE416 pKa = 4.35 LRR418 pKa = 11.84 IQHH421 pKa = 6.55 LSDD424 pKa = 3.68 RR425 pKa = 11.84 VDD427 pKa = 4.29 DD428 pKa = 4.57 ALLDD432 pKa = 3.13 IWSYY436 pKa = 8.75 NTEE439 pKa = 4.04 LLVLLEE445 pKa = 4.23 NEE447 pKa = 4.24 RR448 pKa = 11.84 TLDD451 pKa = 3.57 FHH453 pKa = 7.39 DD454 pKa = 5.04 ANVKK458 pKa = 10.21 NLFEE462 pKa = 4.18 KK463 pKa = 11.01 VKK465 pKa = 10.96 AQLKK469 pKa = 10.6 DD470 pKa = 3.44 NAIDD474 pKa = 3.66 EE475 pKa = 4.81 GNGCFLLLHH484 pKa = 6.57 KK485 pKa = 10.67 CNNSCMDD492 pKa = 4.78 DD493 pKa = 3.59 IKK495 pKa = 11.39 NGTYY499 pKa = 10.34 KK500 pKa = 11.01 YY501 pKa = 9.21 MDD503 pKa = 3.72 YY504 pKa = 10.81 RR505 pKa = 11.84 EE506 pKa = 4.05 EE507 pKa = 4.01 SHH509 pKa = 7.54 IEE511 pKa = 3.71 KK512 pKa = 10.59 QKK514 pKa = 10.49 IDD516 pKa = 3.71 GVKK519 pKa = 8.73 LTDD522 pKa = 3.24 YY523 pKa = 10.68 SRR525 pKa = 11.84 YY526 pKa = 10.63 YY527 pKa = 10.58 IMTLYY532 pKa = 9.59 STIASSVVLGSLIIAAFLWGCQKK555 pKa = 11.09 GSIQCKK561 pKa = 9.13 ICII564 pKa = 4.08
Molecular weight: 63.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.984
IPC2_protein 5.118
IPC_protein 5.08
Toseland 5.029
ProMoST 5.219
Dawson 5.08
Bjellqvist 5.194
Wikipedia 4.94
Rodwell 4.991
Grimsley 4.952
Solomon 5.08
Lehninger 5.029
Nozaki 5.194
DTASelect 5.347
Thurlkill 5.041
EMBOSS 5.003
Sillero 5.27
Patrickios 3.541
IPC_peptide 5.08
IPC2_peptide 5.27
IPC2.peptide.svr19 5.207
Protein with the highest isoelectric point:
>tr|H6QM71|H6QM71_I09A8 RNA-directed RNA polymerase catalytic subunit OS=Influenza A virus (strain A/little yellow-shouldered bat/Guatemala/153/2009(H17N10)) OX=1129345 GN=PB1 PE=4 SV=1
MM1 pKa = 7.33 EE2 pKa = 6.17 RR3 pKa = 11.84 IKK5 pKa = 10.93 EE6 pKa = 3.92 LLEE9 pKa = 3.81 MVKK12 pKa = 10.28 NSRR15 pKa = 11.84 MRR17 pKa = 11.84 EE18 pKa = 3.86 ILTTTSVDD26 pKa = 2.85 HH27 pKa = 5.96 MAVIKK32 pKa = 10.36 KK33 pKa = 7.36 YY34 pKa = 9.12 TSGRR38 pKa = 11.84 QEE40 pKa = 3.99 KK41 pKa = 10.64 NPALRR46 pKa = 11.84 MKK48 pKa = 10.19 WMMAMKK54 pKa = 10.18 YY55 pKa = 9.37 PISASSRR62 pKa = 11.84 IRR64 pKa = 11.84 EE65 pKa = 4.21 MIPEE69 pKa = 4.19 KK70 pKa = 11.09 DD71 pKa = 3.27 EE72 pKa = 5.33 DD73 pKa = 5.52 GNTLWTNTKK82 pKa = 10.09 DD83 pKa = 3.22 AGSNRR88 pKa = 11.84 VLVSPNAVTWWNRR101 pKa = 11.84 AGPVSDD107 pKa = 3.54 VVHH110 pKa = 6.0 YY111 pKa = 9.76 PRR113 pKa = 11.84 VYY115 pKa = 11.01 KK116 pKa = 10.19 MYY118 pKa = 10.25 FDD120 pKa = 3.62 RR121 pKa = 11.84 LEE123 pKa = 4.26 RR124 pKa = 11.84 LTHH127 pKa = 5.26 GTFGPVKK134 pKa = 10.1 FYY136 pKa = 11.42 NQVKK140 pKa = 8.71 VRR142 pKa = 11.84 KK143 pKa = 9.41 RR144 pKa = 11.84 VDD146 pKa = 2.96 INPGHH151 pKa = 7.14 KK152 pKa = 10.4 DD153 pKa = 3.12 LTSRR157 pKa = 11.84 EE158 pKa = 4.1 AQEE161 pKa = 4.57 VIMEE165 pKa = 4.2 VVFPNEE171 pKa = 3.32 VGARR175 pKa = 11.84 TLSSDD180 pKa = 3.38 AQLTITKK187 pKa = 9.41 EE188 pKa = 4.04 KK189 pKa = 10.8 KK190 pKa = 10.23 EE191 pKa = 3.87 EE192 pKa = 3.95 LKK194 pKa = 10.74 NCKK197 pKa = 9.41 ISPIMVAYY205 pKa = 8.87 MLEE208 pKa = 4.23 RR209 pKa = 11.84 EE210 pKa = 4.37 LVRR213 pKa = 11.84 RR214 pKa = 11.84 TRR216 pKa = 11.84 FLPIAGATSSTYY228 pKa = 10.78 VEE230 pKa = 4.48 VLHH233 pKa = 6.22 LTQGTCWEE241 pKa = 4.07 QQYY244 pKa = 8.58 TPGGEE249 pKa = 4.19 AEE251 pKa = 5.25 NDD253 pKa = 3.85 DD254 pKa = 4.81 LDD256 pKa = 3.62 QTLIIASRR264 pKa = 11.84 NIVRR268 pKa = 11.84 RR269 pKa = 11.84 SIVAIDD275 pKa = 3.83 PLASLLSMCHH285 pKa = 4.51 TTSISSEE292 pKa = 3.89 PLVEE296 pKa = 4.06 ILRR299 pKa = 11.84 SNPTDD304 pKa = 3.37 EE305 pKa = 4.35 QAVNICKK312 pKa = 10.0 AALGIRR318 pKa = 11.84 INNSFSFGGYY328 pKa = 7.5 NFKK331 pKa = 10.39 RR332 pKa = 11.84 VKK334 pKa = 10.43 GSSQRR339 pKa = 11.84 TEE341 pKa = 3.87 KK342 pKa = 10.91 AVLTGNLQTLTMTIFEE358 pKa = 4.88 GYY360 pKa = 10.56 EE361 pKa = 3.77 EE362 pKa = 4.93 FNVSGKK368 pKa = 9.99 RR369 pKa = 11.84 ASAVLKK375 pKa = 10.67 KK376 pKa = 9.67 GTQRR380 pKa = 11.84 LIQAIIGGRR389 pKa = 11.84 TLEE392 pKa = 5.21 DD393 pKa = 3.46 ILNLMITLMVFSQEE407 pKa = 3.89 EE408 pKa = 4.23 KK409 pKa = 9.24 MLKK412 pKa = 9.98 AVRR415 pKa = 11.84 GDD417 pKa = 3.59 LNFVNRR423 pKa = 11.84 ANQRR427 pKa = 11.84 LNPMYY432 pKa = 10.24 QLLRR436 pKa = 11.84 HH437 pKa = 5.39 FQKK440 pKa = 10.91 DD441 pKa = 3.04 SSTLLRR447 pKa = 11.84 NWGTEE452 pKa = 3.95 EE453 pKa = 4.2 IDD455 pKa = 4.31 PIMGIAGIMPDD466 pKa = 3.17 GTINKK471 pKa = 8.25 NQTLIGVRR479 pKa = 11.84 LSQGGVDD486 pKa = 3.01 EE487 pKa = 4.71 YY488 pKa = 11.65 SFNEE492 pKa = 4.33 RR493 pKa = 11.84 IRR495 pKa = 11.84 VNIDD499 pKa = 2.37 KK500 pKa = 10.45 YY501 pKa = 11.09 LRR503 pKa = 11.84 VRR505 pKa = 11.84 NEE507 pKa = 3.58 KK508 pKa = 10.96 GEE510 pKa = 4.15 LLISPEE516 pKa = 4.37 EE517 pKa = 3.87 VSEE520 pKa = 4.16 AQGQEE525 pKa = 4.05 KK526 pKa = 10.84 LPINYY531 pKa = 9.2 NSSLMWEE538 pKa = 4.36 VNGPEE543 pKa = 4.26 SILTNTYY550 pKa = 9.85 HH551 pKa = 7.33 WIIKK555 pKa = 9.32 NWEE558 pKa = 4.05 LLKK561 pKa = 9.1 TQWMTDD567 pKa = 3.0 PTVLYY572 pKa = 10.96 NRR574 pKa = 11.84 MEE576 pKa = 4.14 FEE578 pKa = 4.45 PFQTLIPKK586 pKa = 10.04 GNRR589 pKa = 11.84 ATYY592 pKa = 10.54 SGFTRR597 pKa = 11.84 TLFQQMRR604 pKa = 11.84 DD605 pKa = 3.39 VEE607 pKa = 4.57 GTFDD611 pKa = 4.22 SIQVIKK617 pKa = 10.63 LLPFSAHH624 pKa = 6.46 PPSLGRR630 pKa = 11.84 TQFSSFTLNIRR641 pKa = 11.84 GAPLRR646 pKa = 11.84 LLIRR650 pKa = 11.84 GNSQVFNYY658 pKa = 10.42 NQMEE662 pKa = 4.12 NVIIVLGKK670 pKa = 10.2 SVGSPEE676 pKa = 4.2 RR677 pKa = 11.84 SILTEE682 pKa = 3.77 SSSIEE687 pKa = 4.15 SAVLRR692 pKa = 11.84 GFLILGKK699 pKa = 10.26 ANSKK703 pKa = 9.91 YY704 pKa = 10.93 GPVLTIGEE712 pKa = 4.38 LDD714 pKa = 3.14 KK715 pKa = 11.41 LGRR718 pKa = 11.84 GEE720 pKa = 4.21 KK721 pKa = 10.72 ANVLIGQGDD730 pKa = 3.89 TVLVMKK736 pKa = 10.33 RR737 pKa = 11.84 KK738 pKa = 9.31 RR739 pKa = 11.84 DD740 pKa = 3.62 SSILTDD746 pKa = 3.22 SQTALKK752 pKa = 10.24 RR753 pKa = 11.84 IRR755 pKa = 11.84 LEE757 pKa = 3.74 EE758 pKa = 4.22 SKK760 pKa = 11.23
Molecular weight: 86.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.81
IPC2_protein 8.726
IPC_protein 8.639
Toseland 9.428
ProMoST 9.209
Dawson 9.692
Bjellqvist 9.385
Wikipedia 9.867
Rodwell 9.984
Grimsley 9.765
Solomon 9.721
Lehninger 9.677
Nozaki 9.487
DTASelect 9.37
Thurlkill 9.545
EMBOSS 9.867
Sillero 9.633
Patrickios 5.245
IPC_peptide 9.721
IPC2_peptide 7.878
IPC2.peptide.svr19 7.804
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
0
10
4423
96
760
442.3
50.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.63 ± 0.562
1.628 ± 0.315
4.364 ± 0.275
7.619 ± 0.619
4.002 ± 0.292
6.579 ± 0.587
1.515 ± 0.162
7.19 ± 0.197
6.308 ± 0.385
9.202 ± 0.477
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.482 ± 0.324
6.127 ± 0.509
3.798 ± 0.163
4.183 ± 0.282
5.811 ± 0.556
6.851 ± 0.34
6.692 ± 0.422
4.612 ± 0.37
1.515 ± 0.167
2.894 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here