Tistlia consotensis USBA 355

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhodovibrionaceae; Tistlia; Tistlia consotensis

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6388 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y6BC91|A0A1Y6BC91_9PROT Acetolactate synthase large subunit OS=Tistlia consotensis USBA 355 OX=560819 GN=SAMN05428998_103128 PE=3 SV=1
EEE2 pKa = 5.37HH3 pKa = 6.97YY4 pKa = 10.97YY5 pKa = 10.8DD6 pKa = 5.11DD7 pKa = 5.21SGATDDD13 pKa = 4.53LQNSQNLLFDDD24 pKa = 4.67IGANGYYY31 pKa = 9.94DD32 pKa = 3.28NAYYY36 pKa = 10.25NYYY39 pKa = 9.48DD40 pKa = 3.23SLTVTDDD47 pKa = 3.99KK48 pKa = 11.64SGAVVGEEE56 pKa = 3.67DDD58 pKa = 3.44LVRR61 pKa = 11.84VAPDDD66 pKa = 3.01VVAADDD72 pKa = 3.6SGDDD76 pKa = 3.58YY77 pKa = 7.91TIQAAIDDD85 pKa = 3.44AQAGDDD91 pKa = 4.25IVVAAGSYYY100 pKa = 9.56PGLVDDD106 pKa = 4.6YY107 pKa = 9.06TASFGAGRR115 pKa = 11.84GTLNQQILVDDD126 pKa = 4.35KK127 pKa = 10.48LTLIGLPGATIQVAEEE143 pKa = 4.48DDD145 pKa = 3.74TALADDD151 pKa = 3.79TLGFTVAADDD161 pKa = 3.76DD162 pKa = 3.93SISGFEEE169 pKa = 3.84VGPLGDDD176 pKa = 3.79QQDDD180 pKa = 3.31FDDD183 pKa = 3.68ANRR186 pKa = 11.84GYYY189 pKa = 11.03YYY191 pKa = 10.62IFVDDD196 pKa = 4.23EE197 pKa = 4.75VLGTTLSGNTIQDDD211 pKa = 3.48RR212 pKa = 11.84TGITFEEE219 pKa = 4.23ANGDDD224 pKa = 3.99LDDD227 pKa = 4.4SHHH230 pKa = 6.38TSVTGNEEE238 pKa = 3.79DDD240 pKa = 3.54TRR242 pKa = 11.84GAFLVRR248 pKa = 11.84SEEE251 pKa = 4.12VEEE254 pKa = 3.81SDDD257 pKa = 4.35SFGAEEE263 pKa = 3.78NEEE266 pKa = 3.56DDD268 pKa = 4.07TILAGTQPGDDD279 pKa = 3.64YY280 pKa = 10.31ADDD283 pKa = 3.89LVDDD287 pKa = 3.41AQYYY291 pKa = 10.91DDD293 pKa = 4.55DD294 pKa = 3.65MALSTANDDD303 pKa = 2.91MTIVDDD309 pKa = 3.35RR310 pKa = 11.84YYY312 pKa = 10.76EEE314 pKa = 4.55GVLTQQYYY322 pKa = 10.63SSDDD326 pKa = 3.68ALSQQYYY333 pKa = 10.34AIANRR338 pKa = 11.84THHH341 pKa = 7.17EEE343 pKa = 3.92RR344 pKa = 11.84AGADDD349 pKa = 3.61DD350 pKa = 4.0PSAALGEEE358 pKa = 4.34RR359 pKa = 11.84GNGFGSEEE367 pKa = 4.02LPIGSLQDDD376 pKa = 3.35VDDD379 pKa = 3.52VVKKK383 pKa = 10.23GSVHHH388 pKa = 6.48QAGDDD393 pKa = 3.68YY394 pKa = 10.88GEEE397 pKa = 4.24VAVATEEE404 pKa = 3.92LTVDDD409 pKa = 4.13DDD411 pKa = 3.68GAIGIALQLASGIEEE426 pKa = 4.1VTATGAADDD435 pKa = 3.39ALTGNEEE442 pKa = 4.18ANLLAGGEEE451 pKa = 4.2DDD453 pKa = 4.52DD454 pKa = 3.72LFGGGAADDD463 pKa = 3.98LTGGGGNDDD472 pKa = 3.31LVGGEEE478 pKa = 4.5TDDD481 pKa = 3.4AHHH484 pKa = 6.15TGAQADDD491 pKa = 3.95YY492 pKa = 11.02IALNDDD498 pKa = 4.12DD499 pKa = 3.82SFTVADDD506 pKa = 5.02SAGRR510 pKa = 11.84DDD512 pKa = 3.72SDDD515 pKa = 2.91HH516 pKa = 6.8SGVEEE521 pKa = 3.67LAFGDDD527 pKa = 4.21QVLLVGGSGFDDD539 pKa = 3.59LQGAIDDD546 pKa = 3.8ASDDD550 pKa = 3.44DDD552 pKa = 4.29ILVYYY557 pKa = 10.24GQYYY561 pKa = 9.81EEE563 pKa = 4.34ANYYY567 pKa = 9.71PNTGLNDDD575 pKa = 3.57SYYY578 pKa = 11.49GGNNPLGLVIGKKK591 pKa = 9.29VTIQGVDDD599 pKa = 3.4AGHHH603 pKa = 7.59ITDDD607 pKa = 3.64HH608 pKa = 6.61GVEEE612 pKa = 4.22TISATRR618 pKa = 11.84QSNFGTSFLVTADDD632 pKa = 3.22HH633 pKa = 6.26AIHHH637 pKa = 5.88LEEE640 pKa = 3.94LGNSSPNQPYYY651 pKa = 8.28NKKK654 pKa = 9.64IEEE657 pKa = 4.43IADDD661 pKa = 4.1FTLDDD666 pKa = 3.57SVVGAVDDD674 pKa = 3.52YY675 pKa = 11.49HHH677 pKa = 7.38HHH679 pKa = 4.92QPTAADDD686 pKa = 3.8LSGSWDDD693 pKa = 3.31HHH695 pKa = 4.18QMLTAVYYY703 pKa = 10.22NDDD706 pKa = 3.67YYY708 pKa = 10.53TVPSDDD714 pKa = 4.23AGWQSAISSYYY725 pKa = 11.09DD726 pKa = 3.35EEE728 pKa = 4.82SQLAGTIEEE737 pKa = 4.33ASGPGYYY744 pKa = 10.79YYY746 pKa = 10.99ADDD749 pKa = 3.86LDDD752 pKa = 3.54RR753 pKa = 11.84IVGNLFINVAADDD766 pKa = 3.39DD767 pKa = 4.34TWHHH771 pKa = 6.68NGILITGQDDD781 pKa = 3.63EEE783 pKa = 4.65GWRR786 pKa = 11.84LAPAALPTEEE796 pKa = 3.71SGNVFGADDD805 pKa = 2.99DDD807 pKa = 4.51IFWVRR812 pKa = 11.84GEEE815 pKa = 4.31AQDDD819 pKa = 3.96PDDD822 pKa = 3.7AYYY825 pKa = 10.49QSLIANNDDD834 pKa = 3.3PAYYY838 pKa = 9.6YYY840 pKa = 10.34VDDD843 pKa = 4.03SGAPAIGTYYY853 pKa = 10.63DD854 pKa = 3.63DDD856 pKa = 4.5GPLLPGGVPVTPSSPSFAIRR876 pKa = 11.84QSAGDDD882 pKa = 3.43DDD884 pKa = 6.2NEEE887 pKa = 3.88HHH889 pKa = 6.93ADDD892 pKa = 3.4LVVKKK897 pKa = 10.63EEE899 pKa = 3.69DDD901 pKa = 3.15ASRR904 pKa = 11.84TLSLIVGSDDD914 pKa = 3.3ADDD917 pKa = 3.27LSGGSGDDD925 pKa = 3.66EE926 pKa = 4.02LLGGGGDDD934 pKa = 3.97DD935 pKa = 5.2IDDD938 pKa = 3.67GGGNDDD944 pKa = 5.25ILGGAGDDD952 pKa = 4.33DD953 pKa = 3.87LFGGAGNDDD962 pKa = 3.55LAGGDDD968 pKa = 3.61SDDD971 pKa = 3.74VTGGQGDDD979 pKa = 4.18DD980 pKa = 3.04VVFQQGEEE988 pKa = 4.34GSDDD992 pKa = 3.12VTDDD996 pKa = 3.6SGGDDD1001 pKa = 3.68LDDD1004 pKa = 4.18SDDD1007 pKa = 5.19VQSDDD1012 pKa = 4.36PAEEE1016 pKa = 3.92HHH1018 pKa = 7.02GDDD1021 pKa = 4.01TDDD1024 pKa = 2.81HHH1026 pKa = 5.57QVATAAAPEEE1036 pKa = 4.28PLAVVEEE1043 pKa = 4.42VPAASLTVDDD1053 pKa = 2.8HH1054 pKa = 7.57DD1055 pKa = 4.88NVTLTAAA

Molecular weight: 107.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y6CEB2|A0A1Y6CEB2_9PROT Crotonobetaine/carnitine-CoA ligase OS=Tistlia consotensis USBA 355 OX=560819 GN=SAMN05428998_12153 PE=4 SV=1
MM1 pKa = 7.59RR2 pKa = 11.84RR3 pKa = 11.84LRR5 pKa = 11.84RR6 pKa = 11.84LLGLAVAGQPVPRR19 pKa = 11.84THH21 pKa = 7.39GLGLLRR27 pKa = 11.84AAWAGRR33 pKa = 11.84GRR35 pKa = 11.84LLRR38 pKa = 11.84ALAGTIRR45 pKa = 11.84PVRR48 pKa = 11.84RR49 pKa = 11.84GRR51 pKa = 11.84TRR53 pKa = 11.84EE54 pKa = 3.83PRR56 pKa = 11.84PVAGG60 pKa = 4.3

Molecular weight: 6.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6388

0

6388

2078936

25

8553

325.4

35.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.882 ± 0.049

0.896 ± 0.011

5.291 ± 0.023

6.143 ± 0.035

3.474 ± 0.02

9.292 ± 0.029

1.857 ± 0.014

4.004 ± 0.019

2.426 ± 0.025

11.638 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.952 ± 0.016

1.899 ± 0.016

5.697 ± 0.026

2.955 ± 0.017

8.126 ± 0.037

4.856 ± 0.026

4.634 ± 0.03

7.426 ± 0.026

1.424 ± 0.012

2.127 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski