Flavobacterium phage 2A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Unahavirus; Flavobacterium virus 2A

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B0WMM5|A0A1B0WMM5_9CAUD Transcriptional regulator OS=Flavobacterium phage 2A OX=1792273 PE=4 SV=1
MM1 pKa = 8.06RR2 pKa = 11.84NRR4 pKa = 11.84IYY6 pKa = 10.25IQYY9 pKa = 9.89SGKK12 pKa = 8.17ITKK15 pKa = 8.21NTIALMEE22 pKa = 4.56TYY24 pKa = 10.11LICDD28 pKa = 4.78FIGRR32 pKa = 11.84TVIDD36 pKa = 3.57GRR38 pKa = 11.84RR39 pKa = 11.84AYY41 pKa = 9.61IDD43 pKa = 3.9LKK45 pKa = 10.97QSEE48 pKa = 4.73NPNLDD53 pKa = 3.37EE54 pKa = 4.11VSQFILALDD63 pKa = 3.84GTDD66 pKa = 6.66FITFWGYY73 pKa = 9.86PLEE76 pKa = 4.99DD77 pKa = 3.88GSNITTNEE85 pKa = 3.77DD86 pKa = 3.29FKK88 pKa = 11.48DD89 pKa = 4.53FSISEE94 pKa = 3.79IDD96 pKa = 3.12AA97 pKa = 4.2

Molecular weight: 11.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B0WM67|A0A1B0WM67_9CAUD Uncharacterized protein OS=Flavobacterium phage 2A OX=1792273 PE=4 SV=1
MM1 pKa = 7.7FYY3 pKa = 10.51FISATFSTLKK13 pKa = 10.55KK14 pKa = 8.98ITPLKK19 pKa = 10.68SRR21 pKa = 11.84VEE23 pKa = 3.87RR24 pKa = 11.84LRR26 pKa = 11.84DD27 pKa = 3.43KK28 pKa = 10.62KK29 pKa = 11.11KK30 pKa = 11.05KK31 pKa = 8.3MNLYY35 pKa = 9.6IIKK38 pKa = 10.36KK39 pKa = 8.87IFFYY43 pKa = 9.63VQRR46 pKa = 11.84II47 pKa = 3.54

Molecular weight: 5.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

14035

47

1419

230.1

26.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.593 ± 0.503

0.834 ± 0.13

5.479 ± 0.192

7.346 ± 0.209

5.116 ± 0.259

5.023 ± 0.264

1.589 ± 0.159

8.593 ± 0.3

9.576 ± 0.327

8.963 ± 0.268

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.33 ± 0.145

6.819 ± 0.296

2.793 ± 0.167

3.855 ± 0.169

3.769 ± 0.215

6.37 ± 0.261

5.23 ± 0.264

5.365 ± 0.169

1.197 ± 0.115

4.161 ± 0.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski