Streptococcus phage Javan311

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A8A3|A0A4D6A8A3_9CAUD Uncharacterized protein OS=Streptococcus phage Javan311 OX=2548103 GN=Javan311_0013 PE=4 SV=1
MM1 pKa = 7.52SVQQSIVNGFISRR14 pKa = 11.84RR15 pKa = 11.84GLITYY20 pKa = 10.89SMLGSRR26 pKa = 11.84NGSDD30 pKa = 3.22GTGDD34 pKa = 3.27CSGIVSQVLKK44 pKa = 10.35EE45 pKa = 3.88AGINIIGLPSTVTLGQQLANNGFYY69 pKa = 10.41RR70 pKa = 11.84ISRR73 pKa = 11.84NQPWDD78 pKa = 3.6AQPADD83 pKa = 4.51IILMSWGADD92 pKa = 3.24MSSSGGAGGHH102 pKa = 5.27VGAMIDD108 pKa = 3.28DD109 pKa = 4.65TYY111 pKa = 11.13FISCDD116 pKa = 3.44YY117 pKa = 9.43STQGAPGQAINTYY130 pKa = 8.99PWNDD134 pKa = 3.29YY135 pKa = 10.7YY136 pKa = 11.8NWNKK140 pKa = 9.04PAYY143 pKa = 8.8IEE145 pKa = 3.88VWRR148 pKa = 11.84YY149 pKa = 10.53ADD151 pKa = 4.2TAPQTNNQANTAVQPKK167 pKa = 10.28DD168 pKa = 3.24KK169 pKa = 11.01AFYY172 pKa = 9.15EE173 pKa = 4.34ANEE176 pKa = 3.98VKK178 pKa = 10.47YY179 pKa = 10.75INGMWQIKK187 pKa = 9.62CDD189 pKa = 3.65YY190 pKa = 8.74LAPVGFDD197 pKa = 2.79WTEE200 pKa = 3.54NGIPVSMVNWVDD212 pKa = 3.19KK213 pKa = 11.14DD214 pKa = 3.99GNNLPDD220 pKa = 4.95GADD223 pKa = 3.24QDD225 pKa = 4.92FKK227 pKa = 11.67AGMYY231 pKa = 10.24FSFEE235 pKa = 4.18LDD237 pKa = 3.29EE238 pKa = 4.97VNITDD243 pKa = 3.75TGKK246 pKa = 9.39GGYY249 pKa = 9.11YY250 pKa = 10.14GGYY253 pKa = 7.62YY254 pKa = 8.88WRR256 pKa = 11.84LFEE259 pKa = 5.13FGQFGPIWLSCWDD272 pKa = 3.98KK273 pKa = 11.8DD274 pKa = 4.5DD275 pKa = 4.37LVNYY279 pKa = 9.92YY280 pKa = 9.54EE281 pKa = 4.09

Molecular weight: 31.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A9Q7|A0A4D6A9Q7_9CAUD Cytosine-specific methyltransferase OS=Streptococcus phage Javan311 OX=2548103 GN=Javan311_0024 PE=3 SV=1
MM1 pKa = 7.65RR2 pKa = 11.84RR3 pKa = 11.84VQNKK7 pKa = 8.34PRR9 pKa = 11.84EE10 pKa = 4.12ARR12 pKa = 11.84RR13 pKa = 11.84AVSAEE18 pKa = 3.95LQRR21 pKa = 11.84SALRR25 pKa = 11.84VEE27 pKa = 5.57RR28 pKa = 11.84KK29 pKa = 10.18AKK31 pKa = 9.47MKK33 pKa = 10.58AAVDD37 pKa = 3.43TGFMRR42 pKa = 11.84NGIFVARR49 pKa = 11.84VGMLRR54 pKa = 11.84YY55 pKa = 9.63KK56 pKa = 9.07VTSPAGYY63 pKa = 9.3SVYY66 pKa = 10.84VEE68 pKa = 4.04LGTRR72 pKa = 11.84KK73 pKa = 8.95MKK75 pKa = 10.45AQPFLGPAVKK85 pKa = 10.28EE86 pKa = 3.77EE87 pKa = 4.24SEE89 pKa = 4.51ALFKK93 pKa = 10.86NLRR96 pKa = 11.84KK97 pKa = 9.12MFRR100 pKa = 11.84RR101 pKa = 3.83

Molecular weight: 11.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

12142

44

1566

258.3

28.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.005 ± 0.814

0.437 ± 0.09

5.798 ± 0.411

7.964 ± 0.737

3.912 ± 0.219

6.803 ± 0.338

1.285 ± 0.162

6.226 ± 0.238

7.832 ± 0.5

8.499 ± 0.307

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.364 ± 0.253

5.658 ± 0.353

3.154 ± 0.341

4.579 ± 0.441

4.381 ± 0.465

6.136 ± 0.614

6.309 ± 0.453

6.218 ± 0.27

1.137 ± 0.161

3.303 ± 0.415
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski