Coraliomargarita sp. CAG:312
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2060 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R7L6V1|R7L6V1_9BACT UPF0365 protein BN601_00101 OS=Coraliomargarita sp. CAG:312 OX=1262865 GN=BN601_00101 PE=3 SV=1
MM1 pKa = 7.58 TKK3 pKa = 10.49 NKK5 pKa = 10.18 DD6 pKa = 3.48 DD7 pKa = 4.8 LGFLSIMADD16 pKa = 3.08 KK17 pKa = 11.23 NEE19 pKa = 4.0 KK20 pKa = 10.73 NEE22 pKa = 3.89 FNVPGKK28 pKa = 10.45 FYY30 pKa = 11.21 VDD32 pKa = 3.76 SQCIGCALCASTAEE46 pKa = 4.17 GLFAISDD53 pKa = 3.65 TGCAYY58 pKa = 10.12 VAKK61 pKa = 10.37 QPASDD66 pKa = 4.68 EE67 pKa = 4.13 EE68 pKa = 4.44 TALCTEE74 pKa = 4.36 AMEE77 pKa = 4.56 SCPVQAIGDD86 pKa = 3.97 DD87 pKa = 4.36 GEE89 pKa = 4.11
Molecular weight: 9.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.788
IPC2_protein 3.999
IPC_protein 3.91
Toseland 3.719
ProMoST 4.012
Dawson 3.884
Bjellqvist 4.113
Wikipedia 3.808
Rodwell 3.745
Grimsley 3.63
Solomon 3.872
Lehninger 3.821
Nozaki 4.012
DTASelect 4.19
Thurlkill 3.77
EMBOSS 3.821
Sillero 4.024
Patrickios 0.846
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.959
Protein with the highest isoelectric point:
>tr|R7LEZ6|R7LEZ6_9BACT ATP-binding protein Uup OS=Coraliomargarita sp. CAG:312 OX=1262865 GN=uup PE=3 SV=1
MM1 pKa = 7.58 RR2 pKa = 11.84 RR3 pKa = 11.84 LNGVQSVPLYY13 pKa = 11.0 FNMLRR18 pKa = 11.84 GNFFFIQRR26 pKa = 11.84 ILPNNSHH33 pKa = 5.36 AWKK36 pKa = 9.93 RR37 pKa = 11.84 ANSYY41 pKa = 9.97 KK42 pKa = 10.62 VNNAKK47 pKa = 7.29 TQKK50 pKa = 9.77 NARR53 pKa = 11.84 LRR55 pKa = 11.84 ICLHH59 pKa = 6.26 FAPILPHH66 pKa = 5.51 GACRR70 pKa = 11.84 EE71 pKa = 4.12 SFRR74 pKa = 11.84 ACGDD78 pKa = 3.03 WKK80 pKa = 10.7 FRR82 pKa = 11.84 ARR84 pKa = 11.84 QAYY87 pKa = 9.39 AKK89 pKa = 10.42 AHH91 pKa = 6.39 SNYY94 pKa = 8.66 FWQVFPALNSGLDD107 pKa = 3.5 VSWQRR112 pKa = 11.84 EE113 pKa = 3.99 QEE115 pKa = 4.12 SAAEE119 pKa = 4.06 FEE121 pKa = 4.64 CGSWHH126 pKa = 4.9 TTQLNPLWSPVGVRR140 pKa = 11.84 SSQEE144 pKa = 3.83 RR145 pKa = 11.84 AWRR148 pKa = 11.84 SQLGEE153 pKa = 3.88
Molecular weight: 17.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.604
IPC_protein 10.321
Toseland 10.467
ProMoST 10.175
Dawson 10.613
Bjellqvist 10.335
Wikipedia 10.818
Rodwell 10.818
Grimsley 10.672
Solomon 10.701
Lehninger 10.657
Nozaki 10.496
DTASelect 10.321
Thurlkill 10.496
EMBOSS 10.877
Sillero 10.54
Patrickios 10.54
IPC_peptide 10.701
IPC2_peptide 9.56
IPC2.peptide.svr19 8.461
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2060
0
2060
677430
29
3629
328.8
36.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.101 ± 0.068
1.546 ± 0.021
5.563 ± 0.042
6.175 ± 0.043
4.988 ± 0.037
7.323 ± 0.044
1.449 ± 0.019
6.872 ± 0.047
7.049 ± 0.058
8.581 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.624 ± 0.025
4.534 ± 0.048
4.123 ± 0.034
2.548 ± 0.029
4.914 ± 0.039
7.422 ± 0.053
4.421 ± 0.047
6.261 ± 0.044
1.178 ± 0.021
3.311 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here