Emmonsia sp. CAC-2015a
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8745 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B7P0N1|A0A1B7P0N1_9EURO CMGC/CDK/CDK8 protein kinase OS=Emmonsia sp. CAC-2015a OX=1658172 GN=ACJ72_03223 PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 10.5 SNEE5 pKa = 4.08 PLHH8 pKa = 6.72 CILRR12 pKa = 11.84 LEE14 pKa = 4.26 RR15 pKa = 11.84 LLYY18 pKa = 9.01 WRR20 pKa = 11.84 PEE22 pKa = 3.85 LGTTPGHH29 pKa = 7.1 LGEE32 pKa = 4.5 AWEE35 pKa = 4.78 APSGLHH41 pKa = 5.88 RR42 pKa = 11.84 FEE44 pKa = 4.83 SVSILCACSTRR55 pKa = 11.84 ISDD58 pKa = 3.93 YY59 pKa = 11.56 DD60 pKa = 4.65 DD61 pKa = 5.08 GDD63 pKa = 5.23 DD64 pKa = 4.48 YY65 pKa = 12.06 DD66 pKa = 6.42 DD67 pKa = 6.03 YY68 pKa = 12.06 DD69 pKa = 6.67 DD70 pKa = 6.6 DD71 pKa = 7.43 DD72 pKa = 7.21 DD73 pKa = 7.35 DD74 pKa = 5.92 DD75 pKa = 4.76 VVDD78 pKa = 4.15 GGGGGSLVTAA88 pKa = 5.21
Molecular weight: 9.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.91
IPC_protein 3.884
Toseland 3.668
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.859
Rodwell 3.719
Grimsley 3.579
Solomon 3.884
Lehninger 3.846
Nozaki 4.012
DTASelect 4.279
Thurlkill 3.732
EMBOSS 3.859
Sillero 4.012
Patrickios 1.926
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.911
Protein with the highest isoelectric point:
>tr|A0A1B7NLI4|A0A1B7NLI4_9EURO Uncharacterized protein OS=Emmonsia sp. CAC-2015a OX=1658172 GN=ACJ72_08214 PE=4 SV=1
MM1 pKa = 7.12 TLSRR5 pKa = 11.84 LPLTRR10 pKa = 11.84 LSMMVFSSKK19 pKa = 10.3 PMPSTLPHH27 pKa = 5.82 VSAALRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 MPQII39 pKa = 3.48
Molecular weight: 4.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 10.833
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.193
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.974
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.127
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8745
0
8745
3939199
30
5889
450.5
49.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.979 ± 0.021
1.172 ± 0.009
5.625 ± 0.018
6.222 ± 0.027
3.625 ± 0.018
6.735 ± 0.027
2.466 ± 0.012
5.085 ± 0.016
5.022 ± 0.023
8.829 ± 0.03
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.08 ± 0.011
3.99 ± 0.013
6.355 ± 0.029
4.095 ± 0.019
6.294 ± 0.022
8.768 ± 0.034
5.942 ± 0.02
5.773 ± 0.019
1.288 ± 0.011
2.654 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here