Lachnobacterium bovis DSM 14045
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2391 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H3F6N5|A0A1H3F6N5_9FIRM Methylglyoxal synthase OS=Lachnobacterium bovis DSM 14045 OX=1122142 GN=mgsA PE=3 SV=1
MM1 pKa = 7.59 EE2 pKa = 5.94 EE3 pKa = 3.69 ITRR6 pKa = 11.84 KK7 pKa = 9.46 EE8 pKa = 3.68 VWFGYY13 pKa = 10.7 DD14 pKa = 3.82 DD15 pKa = 5.76 LEE17 pKa = 4.42 TTEE20 pKa = 5.19 DD21 pKa = 3.84 SITIEE26 pKa = 4.22 FPNVSGMGWKK36 pKa = 10.23 SYY38 pKa = 8.02 DD39 pKa = 3.3 HH40 pKa = 6.87 KK41 pKa = 10.63 HH42 pKa = 4.33 QQEE45 pKa = 3.97 YY46 pKa = 10.53 DD47 pKa = 3.37 VNYY50 pKa = 10.59 EE51 pKa = 3.78 MTTNHH56 pKa = 7.07 PFPPSWLEE64 pKa = 3.53 IVDD67 pKa = 3.72 YY68 pKa = 11.48 SNLEE72 pKa = 4.04 PYY74 pKa = 9.87 IDD76 pKa = 5.35 EE77 pKa = 5.04 PDD79 pKa = 3.86 CLPNFTFSYY88 pKa = 9.94 EE89 pKa = 4.09 GDD91 pKa = 3.56 VVTLRR96 pKa = 11.84 EE97 pKa = 3.97 FMKK100 pKa = 10.4 KK101 pKa = 9.63 YY102 pKa = 10.71 VFGGLISEE110 pKa = 5.42 ADD112 pKa = 3.75 QEE114 pKa = 4.48 DD115 pKa = 3.82 PSYY118 pKa = 11.06 WEE120 pKa = 4.35 DD121 pKa = 3.07
Molecular weight: 14.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.794
IPC2_protein 3.961
IPC_protein 3.91
Toseland 3.719
ProMoST 3.986
Dawson 3.872
Bjellqvist 4.062
Wikipedia 3.77
Rodwell 3.745
Grimsley 3.63
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.151
Thurlkill 3.757
EMBOSS 3.783
Sillero 4.024
Patrickios 0.744
IPC_peptide 3.859
IPC2_peptide 3.999
IPC2.peptide.svr19 3.925
Protein with the highest isoelectric point:
>tr|A0A1H3MA54|A0A1H3MA54_9FIRM Uncharacterized protein OS=Lachnobacterium bovis DSM 14045 OX=1122142 GN=SAMN02910414_02253 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 8.72 MTFQPKK8 pKa = 8.95 KK9 pKa = 7.58 RR10 pKa = 11.84 QRR12 pKa = 11.84 SKK14 pKa = 9.07 VHH16 pKa = 5.95 GFRR19 pKa = 11.84 KK20 pKa = 9.97 RR21 pKa = 11.84 MSTASGRR28 pKa = 11.84 KK29 pKa = 8.42 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.61 GRR39 pKa = 11.84 KK40 pKa = 8.87 KK41 pKa = 10.59 LSAA44 pKa = 3.95
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.125
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.705
Grimsley 12.91
Solomon 13.349
Lehninger 13.247
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.427
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2391
0
2391
786201
39
5296
328.8
37.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.103 ± 0.053
1.364 ± 0.019
5.988 ± 0.046
7.393 ± 0.059
4.388 ± 0.041
6.14 ± 0.055
1.477 ± 0.021
8.677 ± 0.055
9.077 ± 0.055
8.374 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.793 ± 0.025
5.938 ± 0.045
2.701 ± 0.027
2.823 ± 0.026
3.533 ± 0.029
6.187 ± 0.041
5.065 ± 0.038
6.941 ± 0.036
0.691 ± 0.015
4.346 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here