Delftia phage IME-DE1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Piedvirus; Delftia virus IMEDE1

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F7ILQ6|A0A0F7ILQ6_9CAUD Uncharacterized protein OS=Delftia phage IME-DE1 OX=1647385 PE=4 SV=1
MM1 pKa = 7.64AEE3 pKa = 3.82VSQALMFSAMFLLWMSIPKK22 pKa = 10.31DD23 pKa = 3.66DD24 pKa = 5.49DD25 pKa = 3.72EE26 pKa = 5.8PPTGSAPVVPRR37 pKa = 4.04

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F7IK60|A0A0F7IK60_9CAUD Uncharacterized protein OS=Delftia phage IME-DE1 OX=1647385 PE=4 SV=1
MM1 pKa = 7.07SQSNEE6 pKa = 3.8NFQKK10 pKa = 9.37TRR12 pKa = 11.84KK13 pKa = 7.0TRR15 pKa = 11.84EE16 pKa = 3.91SQGFADD22 pKa = 4.65LQAHH26 pKa = 7.93RR27 pKa = 11.84IRR29 pKa = 11.84KK30 pKa = 8.07QKK32 pKa = 10.04RR33 pKa = 11.84QQAHH37 pKa = 4.57KK38 pKa = 9.66QARR41 pKa = 11.84RR42 pKa = 11.84DD43 pKa = 3.72ALEE46 pKa = 4.0DD47 pKa = 3.31RR48 pKa = 4.9

Molecular weight: 5.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

11528

37

1290

240.2

26.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.234 ± 0.693

0.954 ± 0.155

5.812 ± 0.26

6.358 ± 0.365

3.591 ± 0.268

8.276 ± 0.366

1.917 ± 0.217

4.615 ± 0.201

6.098 ± 0.437

7.981 ± 0.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.403 ± 0.204

3.86 ± 0.267

4.511 ± 0.23

4.502 ± 0.39

5.43 ± 0.183

5.647 ± 0.312

5.283 ± 0.295

6.757 ± 0.238

1.848 ± 0.205

2.923 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski