Sclerotinia sclerotiorum umbra-like virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Calvusvirinae; Umbravirus; unclassified Umbravirus

Average proteome isoelectric point is 9.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A172T0R0|A0A172T0R0_9TOMB Replication-associated protein OS=Sclerotinia sclerotiorum umbra-like virus 1 OX=1435450 PE=4 SV=1
MM1 pKa = 7.16STDD4 pKa = 3.47TKK6 pKa = 9.98QTAHH10 pKa = 6.57RR11 pKa = 11.84CLRR14 pKa = 11.84LQPQAPPRR22 pKa = 11.84DD23 pKa = 3.43RR24 pKa = 11.84RR25 pKa = 11.84YY26 pKa = 10.79LSVGGLTTTVVDD38 pKa = 3.5WRR40 pKa = 11.84LAAHH44 pKa = 6.2PHH46 pKa = 5.26RR47 pKa = 11.84RR48 pKa = 11.84WWLTPNDD55 pKa = 3.0FMMFKK60 pKa = 10.74FLRR63 pKa = 11.84KK64 pKa = 9.71NKK66 pKa = 9.69IEE68 pKa = 4.24STTPDD73 pKa = 4.61PIEE76 pKa = 4.47SFAASAFSLDD86 pKa = 3.56HH87 pKa = 7.58DD88 pKa = 4.67EE89 pKa = 4.94GRR91 pKa = 11.84HH92 pKa = 4.42TPVVRR97 pKa = 11.84LPKK100 pKa = 9.84VVPPVEE106 pKa = 4.29YY107 pKa = 10.62VIKK110 pKa = 10.45DD111 pKa = 3.75YY112 pKa = 11.46LQGASGRR119 pKa = 11.84WDD121 pKa = 3.67VPYY124 pKa = 9.96MVYY127 pKa = 10.41PPLSLAGQPAPPDD140 pKa = 3.5VATSTDD146 pKa = 2.92VDD148 pKa = 3.81VGVVEE153 pKa = 5.0GEE155 pKa = 4.07VKK157 pKa = 10.55GRR159 pKa = 11.84VVVRR163 pKa = 11.84LPAAKK168 pKa = 9.4QVRR171 pKa = 11.84FDD173 pKa = 3.58GRR175 pKa = 11.84QTEE178 pKa = 4.06ASTGSASLEE187 pKa = 3.94KK188 pKa = 10.26KK189 pKa = 10.12KK190 pKa = 10.37PSRR193 pKa = 11.84EE194 pKa = 3.99FKK196 pKa = 10.51PSTKK200 pKa = 10.13YY201 pKa = 10.86GPSVLEE207 pKa = 3.91NEE209 pKa = 4.24RR210 pKa = 11.84GEE212 pKa = 4.46LRR214 pKa = 11.84DD215 pKa = 3.47AEE217 pKa = 4.32FGFRR221 pKa = 11.84LRR223 pKa = 11.84KK224 pKa = 9.56RR225 pKa = 11.84GRR227 pKa = 11.84LTRR230 pKa = 11.84LLEE233 pKa = 4.59SIAKK237 pKa = 9.75RR238 pKa = 11.84DD239 pKa = 3.55VSHH242 pKa = 7.05QSGEE246 pKa = 4.09GGKK249 pKa = 8.49TSEE252 pKa = 3.89QPARR256 pKa = 11.84HH257 pKa = 5.46WLGLITRR264 pKa = 11.84WGGKK268 pKa = 7.97LTVTQRR274 pKa = 11.84VKK276 pKa = 11.22LNNTDD281 pKa = 3.21KK282 pKa = 11.36DD283 pKa = 4.12EE284 pKa = 4.63PSEE287 pKa = 4.09DD288 pKa = 3.47CYY290 pKa = 9.51MTVRR294 pKa = 11.84IGGSVLAASARR305 pKa = 11.84LLAGLVVSATNKK317 pKa = 9.41RR318 pKa = 11.84RR319 pKa = 11.84TRR321 pKa = 11.84EE322 pKa = 3.86LQLALTAKK330 pKa = 10.09ARR332 pKa = 11.84QIATASGMTDD342 pKa = 2.5SYY344 pKa = 11.52LAMVLAGTVALAMRR358 pKa = 11.84VSGPEE363 pKa = 3.53QMANEE368 pKa = 4.22SLGGVSGRR376 pKa = 11.84QSQRR380 pKa = 11.84ASEE383 pKa = 4.92DD384 pKa = 2.65IHH386 pKa = 8.55AGRR389 pKa = 11.84TTSLFRR395 pKa = 11.84QFTRR399 pKa = 11.84AVGDD403 pKa = 3.5VMRR406 pKa = 11.84VEE408 pKa = 4.62CVTGVVLPRR417 pKa = 11.84ATTXWGVSNSGIGVCVGSKK436 pKa = 8.22EE437 pKa = 3.93QHH439 pKa = 6.98KK440 pKa = 9.91IRR442 pKa = 11.84QGARR446 pKa = 11.84MVIPSLDD453 pKa = 3.8TTQHH457 pKa = 5.11SCGPEE462 pKa = 3.41ARR464 pKa = 11.84RR465 pKa = 11.84RR466 pKa = 11.84MYY468 pKa = 10.22RR469 pKa = 11.84VWVPSVDD476 pKa = 4.12GLWAPAVHH484 pKa = 6.91ANCVHH489 pKa = 6.31NEE491 pKa = 3.62EE492 pKa = 4.11AALRR496 pKa = 11.84LRR498 pKa = 11.84TLGPTPADD506 pKa = 3.52PVCPEE511 pKa = 4.12LDD513 pKa = 3.21KK514 pKa = 11.66AFARR518 pKa = 11.84VGVLLSRR525 pKa = 11.84LNIQRR530 pKa = 11.84WTKK533 pKa = 7.95QQVVEE538 pKa = 4.58SYY540 pKa = 10.04SGRR543 pKa = 11.84MRR545 pKa = 11.84QRR547 pKa = 11.84YY548 pKa = 7.14QAALEE553 pKa = 4.22SLVEE557 pKa = 4.09EE558 pKa = 4.68PRR560 pKa = 11.84LSKK563 pKa = 10.56YY564 pKa = 9.81DD565 pKa = 3.41RR566 pKa = 11.84RR567 pKa = 11.84LTGFLKK573 pKa = 10.78AEE575 pKa = 4.3KK576 pKa = 10.08FNPLLKK582 pKa = 10.0QSKK585 pKa = 9.34PRR587 pKa = 11.84MIMCRR592 pKa = 11.84SPRR595 pKa = 11.84FNLEE599 pKa = 3.51LASYY603 pKa = 8.93LKK605 pKa = 10.34PLEE608 pKa = 4.27HH609 pKa = 6.82ALWRR613 pKa = 11.84RR614 pKa = 11.84WKK616 pKa = 9.49YY617 pKa = 10.74GRR619 pKa = 11.84GGVTPTRR626 pKa = 11.84VVGKK630 pKa = 9.87GLNGEE635 pKa = 4.12QRR637 pKa = 11.84ASLIRR642 pKa = 11.84RR643 pKa = 11.84KK644 pKa = 9.07MEE646 pKa = 4.15SVGEE650 pKa = 4.21CTVFEE655 pKa = 4.31IDD657 pKa = 3.21GKK659 pKa = 10.9AFEE662 pKa = 4.22AHH664 pKa = 3.99VTLRR668 pKa = 11.84QVDD671 pKa = 4.52LEE673 pKa = 4.27HH674 pKa = 6.91KK675 pKa = 9.74VYY677 pKa = 10.87KK678 pKa = 10.1KK679 pKa = 10.66VYY681 pKa = 8.42PRR683 pKa = 11.84DD684 pKa = 3.61ARR686 pKa = 11.84LHH688 pKa = 7.04DD689 pKa = 4.3LLQTQRR695 pKa = 11.84KK696 pKa = 8.86LKK698 pKa = 10.31GKK700 pKa = 7.22TLGGIKK706 pKa = 9.97FEE708 pKa = 4.73RR709 pKa = 11.84EE710 pKa = 3.83GCRR713 pKa = 11.84ASGDD717 pKa = 3.86FNTGLGNTLVMGSCVDD733 pKa = 4.29AILRR737 pKa = 11.84SAEE740 pKa = 4.03SALGAFRR747 pKa = 11.84TTFLADD753 pKa = 3.26GDD755 pKa = 4.02NALVFVEE762 pKa = 4.43TSKK765 pKa = 11.66AEE767 pKa = 4.04GVRR770 pKa = 11.84SFFSSMVHH778 pKa = 4.44TVVGQEE784 pKa = 3.95LTVEE788 pKa = 4.27KK789 pKa = 8.87PTTIYY794 pKa = 10.87EE795 pKa = 4.12EE796 pKa = 4.67VVFGQCKK803 pKa = 8.76PCFNGDD809 pKa = 3.22HH810 pKa = 5.59YY811 pKa = 10.27TMVRR815 pKa = 11.84HH816 pKa = 5.9PLKK819 pKa = 9.78TLSNAFSGYY828 pKa = 7.93RR829 pKa = 11.84HH830 pKa = 6.47YY831 pKa = 11.13DD832 pKa = 3.0QSAYY836 pKa = 10.62VGPALKK842 pKa = 10.18AVCRR846 pKa = 11.84AEE848 pKa = 4.18LALSRR853 pKa = 11.84GIPLLEE859 pKa = 4.56AYY861 pKa = 8.7FARR864 pKa = 11.84ALEE867 pKa = 4.03RR868 pKa = 11.84LEE870 pKa = 4.17VYY872 pKa = 10.42RR873 pKa = 11.84DD874 pKa = 3.48LKK876 pKa = 11.12DD877 pKa = 3.49PSVVLEE883 pKa = 3.93DD884 pKa = 3.91RR885 pKa = 11.84LRR887 pKa = 11.84HH888 pKa = 5.43ALAPAYY894 pKa = 9.51AAKK897 pKa = 10.37SSNRR901 pKa = 11.84GVGMDD906 pKa = 4.23ARR908 pKa = 11.84LSFQKK913 pKa = 10.46AWGIGVEE920 pKa = 4.23EE921 pKa = 4.33QILMEE926 pKa = 4.67AEE928 pKa = 4.05LVASVDD934 pKa = 3.04HH935 pKa = 6.91WDD937 pKa = 3.23WTTLRR942 pKa = 11.84EE943 pKa = 4.12DD944 pKa = 3.48VHH946 pKa = 8.37VGDD949 pKa = 5.68GPFADD954 pKa = 3.68TDD956 pKa = 4.01LVEE959 pKa = 4.2NRR961 pKa = 11.84VDD963 pKa = 3.67LFLSGRR969 pKa = 11.84RR970 pKa = 11.84LKK972 pKa = 10.83DD973 pKa = 3.02

Molecular weight: 108.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A172T0R0|A0A172T0R0_9TOMB Replication-associated protein OS=Sclerotinia sclerotiorum umbra-like virus 1 OX=1435450 PE=4 SV=1
MM1 pKa = 7.16STDD4 pKa = 3.47TKK6 pKa = 9.98QTAHH10 pKa = 6.57RR11 pKa = 11.84CLRR14 pKa = 11.84LQPQAPPRR22 pKa = 11.84DD23 pKa = 3.43RR24 pKa = 11.84RR25 pKa = 11.84YY26 pKa = 10.79LSVGGLTTTVVDD38 pKa = 3.5WRR40 pKa = 11.84LAAHH44 pKa = 6.2PHH46 pKa = 5.26RR47 pKa = 11.84RR48 pKa = 11.84WWLTPNDD55 pKa = 3.0FMMFKK60 pKa = 10.74FLRR63 pKa = 11.84KK64 pKa = 9.71NKK66 pKa = 9.69IEE68 pKa = 4.24STTPDD73 pKa = 4.61PIEE76 pKa = 4.47SFAASAFSLDD86 pKa = 3.56HH87 pKa = 7.58DD88 pKa = 4.67EE89 pKa = 4.94GRR91 pKa = 11.84HH92 pKa = 4.42TPVVRR97 pKa = 11.84LPKK100 pKa = 9.84VVPPVEE106 pKa = 4.29YY107 pKa = 10.62VIKK110 pKa = 10.45DD111 pKa = 3.75YY112 pKa = 11.46LQGASGRR119 pKa = 11.84WDD121 pKa = 3.67VPYY124 pKa = 9.96MVYY127 pKa = 10.41PPLSLAGQPAPPDD140 pKa = 3.5VATSTDD146 pKa = 2.92VDD148 pKa = 3.81VGVVEE153 pKa = 5.0GEE155 pKa = 4.07VKK157 pKa = 10.55GRR159 pKa = 11.84VVVRR163 pKa = 11.84LPAAKK168 pKa = 9.4QVRR171 pKa = 11.84FDD173 pKa = 3.58GRR175 pKa = 11.84QTEE178 pKa = 4.06ASTGSASLEE187 pKa = 3.94KK188 pKa = 10.26KK189 pKa = 10.12KK190 pKa = 10.37PSRR193 pKa = 11.84EE194 pKa = 3.99FKK196 pKa = 10.51PSTKK200 pKa = 10.13YY201 pKa = 10.86GPSVLEE207 pKa = 3.91NEE209 pKa = 4.24RR210 pKa = 11.84GEE212 pKa = 4.46LRR214 pKa = 11.84DD215 pKa = 3.47AEE217 pKa = 4.32FGFRR221 pKa = 11.84LRR223 pKa = 11.84KK224 pKa = 9.56RR225 pKa = 11.84GRR227 pKa = 11.84LTRR230 pKa = 11.84LLEE233 pKa = 4.59SIAKK237 pKa = 9.75RR238 pKa = 11.84DD239 pKa = 3.55VSHH242 pKa = 7.05QSGEE246 pKa = 4.09GGKK249 pKa = 8.49TSEE252 pKa = 3.89QPARR256 pKa = 11.84HH257 pKa = 5.46WLGLITRR264 pKa = 11.84WGGKK268 pKa = 7.97LTVTQRR274 pKa = 11.84VKK276 pKa = 11.22LNNTDD281 pKa = 3.21KK282 pKa = 11.36DD283 pKa = 4.12EE284 pKa = 4.63PSEE287 pKa = 4.09DD288 pKa = 3.47CYY290 pKa = 9.51MTVRR294 pKa = 11.84IGGSVLAASARR305 pKa = 11.84LLAGLVVSATNKK317 pKa = 9.41RR318 pKa = 11.84RR319 pKa = 11.84TRR321 pKa = 11.84EE322 pKa = 3.86LQLALTAKK330 pKa = 10.09ARR332 pKa = 11.84QIATASGMTDD342 pKa = 2.5SYY344 pKa = 11.52LAMVLAGTVALAMRR358 pKa = 11.84VSGPEE363 pKa = 3.53QMANEE368 pKa = 4.22SLGGVSGRR376 pKa = 11.84QSQRR380 pKa = 11.84ASEE383 pKa = 4.92DD384 pKa = 2.65IHH386 pKa = 8.55AGRR389 pKa = 11.84TTSLFRR395 pKa = 11.84QFTRR399 pKa = 11.84AVGDD403 pKa = 3.5VMRR406 pKa = 11.84VEE408 pKa = 4.62CVTGVVLPRR417 pKa = 11.84ATTT420 pKa = 3.33

Molecular weight: 46.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1393

420

973

696.5

77.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.615 ± 0.107

1.22 ± 0.278

4.953 ± 0.026

6.03 ± 0.304

3.015 ± 0.217

7.825 ± 0.018

2.225 ± 0.176

2.082 ± 0.097

5.169 ± 0.093

9.476 ± 0.366

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.225 ± 0.085

2.01 ± 0.188

5.24 ± 0.39

3.589 ± 0.121

9.691 ± 0.169

6.892 ± 0.268

6.748 ± 0.739

9.117 ± 0.093

1.508 ± 0.043

2.297 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski