Amino acid dipepetide frequency for Sphingomonas sp. WZY 27

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.21AlaAla: 22.21 ± 0.192
1.046AlaCys: 1.046 ± 0.033
7.862AlaAsp: 7.862 ± 0.084
8.414AlaGlu: 8.414 ± 0.102
4.407AlaPhe: 4.407 ± 0.062
12.968AlaGly: 12.968 ± 0.14
2.317AlaHis: 2.317 ± 0.048
6.482AlaIle: 6.482 ± 0.091
3.561AlaLys: 3.561 ± 0.068
14.933AlaLeu: 14.933 ± 0.166
3.652AlaMet: 3.652 ± 0.068
3.147AlaAsn: 3.147 ± 0.062
7.346AlaPro: 7.346 ± 0.117
4.857AlaGln: 4.857 ± 0.08
11.246AlaArg: 11.246 ± 0.13
6.625AlaSer: 6.625 ± 0.074
7.274AlaThr: 7.274 ± 0.105
9.612AlaVal: 9.612 ± 0.124
1.785AlaTrp: 1.785 ± 0.043
2.535AlaTyr: 2.535 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
0.949CysAla: 0.949 ± 0.031
0.089CysCys: 0.089 ± 0.01
0.488CysAsp: 0.488 ± 0.022
0.344CysGlu: 0.344 ± 0.017
0.258CysPhe: 0.258 ± 0.015
0.8CysGly: 0.8 ± 0.032
0.196CysHis: 0.196 ± 0.013
0.311CysIle: 0.311 ± 0.018
0.111CysLys: 0.111 ± 0.009
0.697CysLeu: 0.697 ± 0.027
0.135CysMet: 0.135 ± 0.012
0.175CysAsn: 0.175 ± 0.014
0.428CysPro: 0.428 ± 0.02
0.178CysGln: 0.178 ± 0.014
0.552CysArg: 0.552 ± 0.025
0.416CysSer: 0.416 ± 0.022
0.397CysThr: 0.397 ± 0.017
0.548CysVal: 0.548 ± 0.023
0.105CysTrp: 0.105 ± 0.009
0.149CysTyr: 0.149 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
7.993AspAla: 7.993 ± 0.102
0.419AspCys: 0.419 ± 0.022
2.929AspAsp: 2.929 ± 0.054
3.229AspGlu: 3.229 ± 0.06
2.018AspPhe: 2.018 ± 0.047
5.343AspGly: 5.343 ± 0.089
1.216AspHis: 1.216 ± 0.037
2.221AspIle: 2.221 ± 0.048
1.494AspLys: 1.494 ± 0.039
5.808AspLeu: 5.808 ± 0.079
1.297AspMet: 1.297 ± 0.038
1.196AspAsn: 1.196 ± 0.036
3.762AspPro: 3.762 ± 0.059
1.773AspGln: 1.773 ± 0.046
5.145AspArg: 5.145 ± 0.081
1.912AspSer: 1.912 ± 0.046
2.802AspThr: 2.802 ± 0.051
4.32AspVal: 4.32 ± 0.074
1.115AspTrp: 1.115 ± 0.031
1.453AspTyr: 1.453 ± 0.045
0.0AspXaa: 0.0 ± 0.0
Glu
8.25GluAla: 8.25 ± 0.117
0.279GluCys: 0.279 ± 0.018
2.606GluAsp: 2.606 ± 0.05
3.086GluGlu: 3.086 ± 0.065
1.333GluPhe: 1.333 ± 0.035
4.48GluGly: 4.48 ± 0.077
1.164GluHis: 1.164 ± 0.037
2.503GluIle: 2.503 ± 0.052
1.588GluLys: 1.588 ± 0.044
5.074GluLeu: 5.074 ± 0.072
1.204GluMet: 1.204 ± 0.039
1.236GluAsn: 1.236 ± 0.035
2.745GluPro: 2.745 ± 0.058
2.221GluGln: 2.221 ± 0.054
5.341GluArg: 5.341 ± 0.078
1.906GluSer: 1.906 ± 0.044
3.06GluThr: 3.06 ± 0.053
3.694GluVal: 3.694 ± 0.063
0.748GluTrp: 0.748 ± 0.025
0.895GluTyr: 0.895 ± 0.032
0.0GluXaa: 0.0 ± 0.0
Phe
4.859PheAla: 4.859 ± 0.084
0.313PheCys: 0.313 ± 0.016
2.541PheAsp: 2.541 ± 0.051
1.903PheGlu: 1.903 ± 0.042
1.247PhePhe: 1.247 ± 0.038
3.566PheGly: 3.566 ± 0.058
0.688PheHis: 0.688 ± 0.026
1.051PheIle: 1.051 ± 0.029
0.732PheLys: 0.732 ± 0.025
3.211PheLeu: 3.211 ± 0.062
0.58PheMet: 0.58 ± 0.026
0.911PheAsn: 0.911 ± 0.038
1.44PhePro: 1.44 ± 0.038
0.905PheGln: 0.905 ± 0.025
2.341PheArg: 2.341 ± 0.048
1.845PheSer: 1.845 ± 0.042
2.011PheThr: 2.011 ± 0.04
2.641PheVal: 2.641 ± 0.05
0.521PheTrp: 0.521 ± 0.027
0.803PheTyr: 0.803 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
11.014GlyAla: 11.014 ± 0.13
0.826GlyCys: 0.826 ± 0.033
4.947GlyAsp: 4.947 ± 0.067
5.114GlyGlu: 5.114 ± 0.073
3.536GlyPhe: 3.536 ± 0.052
8.509GlyGly: 8.509 ± 0.123
1.817GlyHis: 1.817 ± 0.04
4.093GlyIle: 4.093 ± 0.066
2.901GlyLys: 2.901 ± 0.066
8.728GlyLeu: 8.728 ± 0.099
2.225GlyMet: 2.225 ± 0.054
2.116GlyAsn: 2.116 ± 0.058
3.565GlyPro: 3.565 ± 0.056
3.043GlyGln: 3.043 ± 0.055
7.314GlyArg: 7.314 ± 0.091
4.896GlySer: 4.896 ± 0.085
5.434GlyThr: 5.434 ± 0.101
6.588GlyVal: 6.588 ± 0.085
1.731GlyTrp: 1.731 ± 0.048
2.277GlyTyr: 2.277 ± 0.051
0.0GlyXaa: 0.0 ± 0.0
His
2.531HisAla: 2.531 ± 0.053
0.183HisCys: 0.183 ± 0.013
1.137HisAsp: 1.137 ± 0.032
0.969HisGlu: 0.969 ± 0.035
0.695HisPhe: 0.695 ± 0.026
1.981HisGly: 1.981 ± 0.051
0.547HisHis: 0.547 ± 0.022
0.65HisIle: 0.65 ± 0.023
0.388HisLys: 0.388 ± 0.022
1.962HisLeu: 1.962 ± 0.047
0.379HisMet: 0.379 ± 0.02
0.413HisAsn: 0.413 ± 0.017
1.309HisPro: 1.309 ± 0.038
0.56HisGln: 0.56 ± 0.023
1.575HisArg: 1.575 ± 0.041
0.794HisSer: 0.794 ± 0.031
0.725HisThr: 0.725 ± 0.025
1.502HisVal: 1.502 ± 0.04
0.365HisTrp: 0.365 ± 0.014
0.485HisTyr: 0.485 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
7.415IleAla: 7.415 ± 0.103
0.331IleCys: 0.331 ± 0.018
3.417IleAsp: 3.417 ± 0.052
2.93IleGlu: 2.93 ± 0.056
1.237IlePhe: 1.237 ± 0.043
4.875IleGly: 4.875 ± 0.086
0.713IleHis: 0.713 ± 0.028
1.316IleIle: 1.316 ± 0.042
0.92IleLys: 0.92 ± 0.035
3.467IleLeu: 3.467 ± 0.061
0.616IleMet: 0.616 ± 0.022
1.135IleAsn: 1.135 ± 0.034
1.789IlePro: 1.789 ± 0.042
0.949IleGln: 0.949 ± 0.031
2.861IleArg: 2.861 ± 0.053
2.028IleSer: 2.028 ± 0.049
2.301IleThr: 2.301 ± 0.047
3.783IleVal: 3.783 ± 0.069
0.488IleTrp: 0.488 ± 0.022
0.809IleTyr: 0.809 ± 0.028
0.0IleXaa: 0.0 ± 0.0
Lys
3.628LysAla: 3.628 ± 0.068
0.132LysCys: 0.132 ± 0.012
1.246LysAsp: 1.246 ± 0.039
1.025LysGlu: 1.025 ± 0.041
0.66LysPhe: 0.66 ± 0.029
2.305LysGly: 2.305 ± 0.055
0.424LysHis: 0.424 ± 0.019
1.13LysIle: 1.13 ± 0.034
0.921LysLys: 0.921 ± 0.038
2.726LysLeu: 2.726 ± 0.057
0.603LysMet: 0.603 ± 0.026
0.642LysAsn: 0.642 ± 0.023
1.769LysPro: 1.769 ± 0.048
0.827LysGln: 0.827 ± 0.029
2.164LysArg: 2.164 ± 0.045
1.299LysSer: 1.299 ± 0.035
1.43LysThr: 1.43 ± 0.042
1.961LysVal: 1.961 ± 0.046
0.299LysTrp: 0.299 ± 0.016
0.481LysTyr: 0.481 ± 0.022
0.0LysXaa: 0.0 ± 0.0
Leu
15.677LeuAla: 15.677 ± 0.158
0.732LeuCys: 0.732 ± 0.027
6.261LeuAsp: 6.261 ± 0.092
4.687LeuGlu: 4.687 ± 0.079
3.603LeuPhe: 3.603 ± 0.065
8.493LeuGly: 8.493 ± 0.102
1.905LeuHis: 1.905 ± 0.051
4.067LeuIle: 4.067 ± 0.065
2.762LeuLys: 2.762 ± 0.053
10.358LeuLeu: 10.358 ± 0.136
1.893LeuMet: 1.893 ± 0.038
2.359LeuAsn: 2.359 ± 0.047
6.033LeuPro: 6.033 ± 0.077
2.649LeuGln: 2.649 ± 0.046
7.44LeuArg: 7.44 ± 0.1
5.773LeuSer: 5.773 ± 0.07
5.597LeuThr: 5.597 ± 0.074
7.802LeuVal: 7.802 ± 0.104
1.259LeuTrp: 1.259 ± 0.039
1.94LeuTyr: 1.94 ± 0.043
0.0LeuXaa: 0.0 ± 0.0
Met
3.1MetAla: 3.1 ± 0.06
0.117MetCys: 0.117 ± 0.011
0.978MetAsp: 0.978 ± 0.032
0.908MetGlu: 0.908 ± 0.032
0.629MetPhe: 0.629 ± 0.022
1.659MetGly: 1.659 ± 0.044
0.388MetHis: 0.388 ± 0.019
1.111MetIle: 1.111 ± 0.035
0.768MetLys: 0.768 ± 0.028
2.375MetLeu: 2.375 ± 0.048
0.534MetMet: 0.534 ± 0.025
0.572MetAsn: 0.572 ± 0.022
1.361MetPro: 1.361 ± 0.037
0.608MetGln: 0.608 ± 0.028
1.728MetArg: 1.728 ± 0.041
1.265MetSer: 1.265 ± 0.035
1.508MetThr: 1.508 ± 0.038
1.497MetVal: 1.497 ± 0.038
0.212MetTrp: 0.212 ± 0.014
0.242MetTyr: 0.242 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.141AsnAla: 3.141 ± 0.062
0.196AsnCys: 0.196 ± 0.014
1.264AsnAsp: 1.264 ± 0.039
1.043AsnGlu: 1.043 ± 0.031
0.826AsnPhe: 0.826 ± 0.03
2.494AsnGly: 2.494 ± 0.061
0.424AsnHis: 0.424 ± 0.022
1.086AsnIle: 1.086 ± 0.037
0.584AsnLys: 0.584 ± 0.024
2.381AsnLeu: 2.381 ± 0.049
0.434AsnMet: 0.434 ± 0.021
0.714AsnAsn: 0.714 ± 0.044
1.635AsnPro: 1.635 ± 0.044
0.709AsnGln: 0.709 ± 0.026
1.823AsnArg: 1.823 ± 0.05
1.124AsnSer: 1.124 ± 0.04
1.183AsnThr: 1.183 ± 0.043
1.747AsnVal: 1.747 ± 0.05
0.408AsnTrp: 0.408 ± 0.018
0.648AsnTyr: 0.648 ± 0.029
0.0AsnXaa: 0.0 ± 0.0
Pro
8.526ProAla: 8.526 ± 0.121
0.326ProCys: 0.326 ± 0.018
3.772ProAsp: 3.772 ± 0.067
3.451ProGlu: 3.451 ± 0.061
1.868ProPhe: 1.868 ± 0.037
5.173ProGly: 5.173 ± 0.075
1.062ProHis: 1.062 ± 0.032
2.332ProIle: 2.332 ± 0.042
1.327ProLys: 1.327 ± 0.041
5.162ProLeu: 5.162 ± 0.079
1.08ProMet: 1.08 ± 0.035
1.257ProAsn: 1.257 ± 0.036
3.094ProPro: 3.094 ± 0.074
1.762ProGln: 1.762 ± 0.043
3.517ProArg: 3.517 ± 0.065
2.715ProSer: 2.715 ± 0.055
2.675ProThr: 2.675 ± 0.049
4.516ProVal: 4.516 ± 0.07
0.693ProTrp: 0.693 ± 0.027
1.032ProTyr: 1.032 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
4.558GlnAla: 4.558 ± 0.069
0.176GlnCys: 0.176 ± 0.013
1.336GlnAsp: 1.336 ± 0.036
1.348GlnGlu: 1.348 ± 0.037
1.049GlnPhe: 1.049 ± 0.032
2.64GlnGly: 2.64 ± 0.047
0.578GlnHis: 0.578 ± 0.024
1.541GlnIle: 1.541 ± 0.038
0.819GlnLys: 0.819 ± 0.032
3.182GlnLeu: 3.182 ± 0.055
0.756GlnMet: 0.756 ± 0.028
0.756GlnAsn: 0.756 ± 0.027
1.94GlnPro: 1.94 ± 0.041
1.2GlnGln: 1.2 ± 0.042
2.746GlnArg: 2.746 ± 0.052
1.577GlnSer: 1.577 ± 0.039
1.582GlnThr: 1.582 ± 0.041
2.668GlnVal: 2.668 ± 0.043
0.419GlnTrp: 0.419 ± 0.022
0.588GlnTyr: 0.588 ± 0.029
0.0GlnXaa: 0.0 ± 0.0
Arg
10.215ArgAla: 10.215 ± 0.119
0.551ArgCys: 0.551 ± 0.023
4.382ArgAsp: 4.382 ± 0.072
4.413ArgGlu: 4.413 ± 0.071
3.327ArgPhe: 3.327 ± 0.064
5.7ArgGly: 5.7 ± 0.08
1.778ArgHis: 1.778 ± 0.044
3.813ArgIle: 3.813 ± 0.059
1.753ArgLys: 1.753 ± 0.045
9.004ArgLeu: 9.004 ± 0.116
1.929ArgMet: 1.929 ± 0.041
1.77ArgAsn: 1.77 ± 0.045
4.325ArgPro: 4.325 ± 0.067
2.721ArgGln: 2.721 ± 0.056
6.811ArgArg: 6.811 ± 0.107
3.869ArgSer: 3.869 ± 0.065
4.104ArgThr: 4.104 ± 0.068
5.463ArgVal: 5.463 ± 0.082
1.427ArgTrp: 1.427 ± 0.04
1.932ArgTyr: 1.932 ± 0.05
0.0ArgXaa: 0.0 ± 0.0
Ser
6.549SerAla: 6.549 ± 0.07
0.375SerCys: 0.375 ± 0.019
2.849SerAsp: 2.849 ± 0.06
2.217SerGlu: 2.217 ± 0.043
2.019SerPhe: 2.019 ± 0.045
5.313SerGly: 5.313 ± 0.073
0.852SerHis: 0.852 ± 0.028
2.344SerIle: 2.344 ± 0.047
1.131SerLys: 1.131 ± 0.031
4.769SerLeu: 4.769 ± 0.074
1.026SerMet: 1.026 ± 0.031
1.287SerAsn: 1.287 ± 0.044
2.904SerPro: 2.904 ± 0.056
1.376SerGln: 1.376 ± 0.041
3.573SerArg: 3.573 ± 0.064
2.541SerSer: 2.541 ± 0.054
2.67SerThr: 2.67 ± 0.055
3.604SerVal: 3.604 ± 0.057
0.781SerTrp: 0.781 ± 0.027
1.264SerTyr: 1.264 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
6.909ThrAla: 6.909 ± 0.108
0.385ThrCys: 0.385 ± 0.019
2.941ThrAsp: 2.941 ± 0.059
2.231ThrGlu: 2.231 ± 0.044
1.904ThrPhe: 1.904 ± 0.044
5.672ThrGly: 5.672 ± 0.092
0.924ThrHis: 0.924 ± 0.033
2.807ThrIle: 2.807 ± 0.056
1.132ThrLys: 1.132 ± 0.038
6.148ThrLeu: 6.148 ± 0.074
1.093ThrMet: 1.093 ± 0.035
1.304ThrAsn: 1.304 ± 0.047
3.699ThrPro: 3.699 ± 0.065
1.578ThrGln: 1.578 ± 0.041
3.879ThrArg: 3.879 ± 0.053
2.628ThrSer: 2.628 ± 0.056
2.771ThrThr: 2.771 ± 0.061
4.491ThrVal: 4.491 ± 0.084
0.646ThrTrp: 0.646 ± 0.026
1.21ThrTyr: 1.21 ± 0.038
0.0ThrXaa: 0.0 ± 0.0
Val
10.726ValAla: 10.726 ± 0.127
0.531ValCys: 0.531 ± 0.023
4.345ValAsp: 4.345 ± 0.067
4.474ValGlu: 4.474 ± 0.082
2.198ValPhe: 2.198 ± 0.049
5.655ValGly: 5.655 ± 0.085
1.339ValHis: 1.339 ± 0.033
3.268ValIle: 3.268 ± 0.058
1.868ValLys: 1.868 ± 0.044
7.471ValLeu: 7.471 ± 0.092
1.424ValMet: 1.424 ± 0.032
1.88ValAsn: 1.88 ± 0.049
4.454ValPro: 4.454 ± 0.068
2.203ValGln: 2.203 ± 0.061
5.755ValArg: 5.755 ± 0.078
4.21ValSer: 4.21 ± 0.063
4.844ValThr: 4.844 ± 0.093
5.768ValVal: 5.768 ± 0.082
0.831ValTrp: 0.831 ± 0.031
1.307ValTyr: 1.307 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
1.433TrpAla: 1.433 ± 0.041
0.129TrpCys: 0.129 ± 0.011
0.681TrpAsp: 0.681 ± 0.027
0.573TrpGlu: 0.573 ± 0.028
0.576TrpPhe: 0.576 ± 0.025
1.003TrpGly: 1.003 ± 0.032
0.348TrpHis: 0.348 ± 0.019
0.617TrpIle: 0.617 ± 0.024
0.404TrpLys: 0.404 ± 0.018
1.772TrpLeu: 1.772 ± 0.045
0.336TrpMet: 0.336 ± 0.018
0.416TrpAsn: 0.416 ± 0.022
0.735TrpPro: 0.735 ± 0.028
0.655TrpGln: 0.655 ± 0.026
1.494TrpArg: 1.494 ± 0.038
0.932TrpSer: 0.932 ± 0.028
0.925TrpThr: 0.925 ± 0.028
0.836TrpVal: 0.836 ± 0.028
0.302TrpTrp: 0.302 ± 0.016
0.297TrpTyr: 0.297 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.596TyrAla: 2.596 ± 0.052
0.192TyrCys: 0.192 ± 0.012
1.462TyrAsp: 1.462 ± 0.043
1.058TyrGlu: 1.058 ± 0.03
0.721TyrPhe: 0.721 ± 0.026
2.046TyrGly: 2.046 ± 0.052
0.468TyrHis: 0.468 ± 0.019
0.639TyrIle: 0.639 ± 0.024
0.506TyrLys: 0.506 ± 0.024
2.059TyrLeu: 2.059 ± 0.046
0.364TyrMet: 0.364 ± 0.021
0.579TyrAsn: 0.579 ± 0.026
1.002TyrPro: 1.002 ± 0.032
0.685TyrGln: 0.685 ± 0.025
1.985TyrArg: 1.985 ± 0.045
1.09TyrSer: 1.09 ± 0.039
1.059TyrThr: 1.059 ± 0.037
1.506TyrVal: 1.506 ± 0.037
0.329TyrTrp: 0.329 ± 0.018
0.592TyrTyr: 0.592 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3410 proteins (1085316 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski