Arthrobacter phage Maja
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S9UN09|A0A3S9UN09_9CAUD Esterase OS=Arthrobacter phage Maja OX=2499009 GN=26 PE=4 SV=1
MM1 pKa = 7.62 TDD3 pKa = 3.42 YY4 pKa = 11.21 PFDD7 pKa = 4.85 NILAVDD13 pKa = 3.97 ASGPEE18 pKa = 4.13 LLGGLPVIRR27 pKa = 11.84 VASNALVTLFDD38 pKa = 4.11 VNDD41 pKa = 3.68 ATKK44 pKa = 10.28 TPVALKK50 pKa = 9.49 TVDD53 pKa = 3.85 GLPIPNPVQVNALGMGPAPVAQFWQIGWEE82 pKa = 4.25 GGGLSGVFTSNLGLRR97 pKa = 11.84 NEE99 pKa = 4.18 TLAGVEE105 pKa = 4.04 AAQAAQLAAEE115 pKa = 4.54 NAASTAGTEE124 pKa = 3.84 AAAAASAALAGAVSDD139 pKa = 4.07 AQSARR144 pKa = 11.84 ADD146 pKa = 3.57 AEE148 pKa = 3.84 AAAALVGAPAGAAIAAAINGNPEE171 pKa = 3.89 VQGEE175 pKa = 4.22 LSATYY180 pKa = 10.65 ASAFPDD186 pKa = 3.73 SQAITYY192 pKa = 9.38 NADD195 pKa = 2.98 GSVATVTEE203 pKa = 4.17 NGVTTTYY210 pKa = 10.28 TYY212 pKa = 11.47 NSDD215 pKa = 3.23 GTVATDD221 pKa = 3.1 TRR223 pKa = 11.84 AGVTRR228 pKa = 11.84 TYY230 pKa = 10.53 TYY232 pKa = 11.22 TNGNLTGIEE241 pKa = 4.02 AAA243 pKa = 4.5
Molecular weight: 24.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.978
IPC2_protein 3.795
IPC_protein 3.745
Toseland 3.541
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.579
Grimsley 3.465
Solomon 3.719
Lehninger 3.668
Nozaki 3.859
DTASelect 4.05
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.872
Patrickios 2.842
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A3S9UNG9|A0A3S9UNG9_9CAUD Uncharacterized protein OS=Arthrobacter phage Maja OX=2499009 GN=38 PE=4 SV=1
MM1 pKa = 7.83 GDD3 pKa = 3.19 HH4 pKa = 6.54 SAAPSSTQRR13 pKa = 11.84 RR14 pKa = 11.84 FPWRR18 pKa = 11.84 ATLRR22 pKa = 11.84 TAFQAGTALAAGAPLIYY39 pKa = 10.08 QAATDD44 pKa = 4.56 HH45 pKa = 7.13 DD46 pKa = 4.23 PAAATGAAGVALAVAAGITRR66 pKa = 11.84 VMALPTVDD74 pKa = 3.0 TFIRR78 pKa = 11.84 RR79 pKa = 11.84 FLPFLAPDD87 pKa = 3.65 NKK89 pKa = 9.46 GTPP92 pKa = 3.54
Molecular weight: 9.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.589
IPC_protein 10.789
Toseland 10.774
ProMoST 10.877
Dawson 10.847
Bjellqvist 10.701
Wikipedia 11.184
Rodwell 10.73
Grimsley 10.906
Solomon 11.14
Lehninger 11.082
Nozaki 10.745
DTASelect 10.701
Thurlkill 10.774
EMBOSS 11.213
Sillero 10.804
Patrickios 10.657
IPC_peptide 11.14
IPC2_peptide 9.911
IPC2.peptide.svr19 8.737
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
56
0
56
11839
43
1176
211.4
22.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.771 ± 0.399
0.718 ± 0.129
5.896 ± 0.3
5.685 ± 0.425
2.982 ± 0.289
8.683 ± 0.477
1.639 ± 0.172
4.612 ± 0.261
4.198 ± 0.23
8.345 ± 0.322
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.331 ± 0.14
2.965 ± 0.213
5.744 ± 0.29
4.088 ± 0.211
6.36 ± 0.549
5.355 ± 0.277
6.234 ± 0.405
7.315 ± 0.4
1.934 ± 0.16
2.145 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here