Arthrobacter phage Maja

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Majavirus; Arthrobacter virus Maja

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A3S9UN09|A0A3S9UN09_9CAUD Esterase OS=Arthrobacter phage Maja OX=2499009 GN=26 PE=4 SV=1
MM1 pKa = 7.62TDD3 pKa = 3.42YY4 pKa = 11.21PFDD7 pKa = 4.85NILAVDD13 pKa = 3.97ASGPEE18 pKa = 4.13LLGGLPVIRR27 pKa = 11.84VASNALVTLFDD38 pKa = 4.11VNDD41 pKa = 3.68ATKK44 pKa = 10.28TPVALKK50 pKa = 9.49TVDD53 pKa = 3.85GLPIPNPVQVNALGMGPAPVAQFWQIGWEE82 pKa = 4.25GGGLSGVFTSNLGLRR97 pKa = 11.84NEE99 pKa = 4.18TLAGVEE105 pKa = 4.04AAQAAQLAAEE115 pKa = 4.54NAASTAGTEE124 pKa = 3.84AAAAASAALAGAVSDD139 pKa = 4.07AQSARR144 pKa = 11.84ADD146 pKa = 3.57AEE148 pKa = 3.84AAAALVGAPAGAAIAAAINGNPEE171 pKa = 3.89VQGEE175 pKa = 4.22LSATYY180 pKa = 10.65ASAFPDD186 pKa = 3.73SQAITYY192 pKa = 9.38NADD195 pKa = 2.98GSVATVTEE203 pKa = 4.17NGVTTTYY210 pKa = 10.28TYY212 pKa = 11.47NSDD215 pKa = 3.23GTVATDD221 pKa = 3.1TRR223 pKa = 11.84AGVTRR228 pKa = 11.84TYY230 pKa = 10.53TYY232 pKa = 11.22TNGNLTGIEE241 pKa = 4.02AAA243 pKa = 4.5

Molecular weight:
24.05 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A3S9UNG9|A0A3S9UNG9_9CAUD Uncharacterized protein OS=Arthrobacter phage Maja OX=2499009 GN=38 PE=4 SV=1
MM1 pKa = 7.83GDD3 pKa = 3.19HH4 pKa = 6.54SAAPSSTQRR13 pKa = 11.84RR14 pKa = 11.84FPWRR18 pKa = 11.84ATLRR22 pKa = 11.84TAFQAGTALAAGAPLIYY39 pKa = 10.08QAATDD44 pKa = 4.56HH45 pKa = 7.13DD46 pKa = 4.23PAAATGAAGVALAVAAGITRR66 pKa = 11.84VMALPTVDD74 pKa = 3.0TFIRR78 pKa = 11.84RR79 pKa = 11.84FLPFLAPDD87 pKa = 3.65NKK89 pKa = 9.46GTPP92 pKa = 3.54

Molecular weight:
9.48 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

11839

43

1176

211.4

22.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.771 ± 0.399

0.718 ± 0.129

5.896 ± 0.3

5.685 ± 0.425

2.982 ± 0.289

8.683 ± 0.477

1.639 ± 0.172

4.612 ± 0.261

4.198 ± 0.23

8.345 ± 0.322

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.331 ± 0.14

2.965 ± 0.213

5.744 ± 0.29

4.088 ± 0.211

6.36 ± 0.549

5.355 ± 0.277

6.234 ± 0.405

7.315 ± 0.4

1.934 ± 0.16

2.145 ± 0.19

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski