Streptococcus phage Javan407

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AC41|A0A4D6AC41_9CAUD Uncharacterized protein OS=Streptococcus phage Javan407 OX=2548153 GN=Javan407_0033 PE=4 SV=1
MM1 pKa = 7.35IPKK4 pKa = 9.7FRR6 pKa = 11.84AWGKK10 pKa = 9.13NGNYY14 pKa = 9.6PGTPSEE20 pKa = 4.27KK21 pKa = 9.8FEE23 pKa = 3.95MFYY26 pKa = 11.01DD27 pKa = 3.81VSIVTTYY34 pKa = 10.13QDD36 pKa = 3.45KK37 pKa = 8.86EE38 pKa = 3.91QHH40 pKa = 6.03VIADD44 pKa = 3.82FGMYY48 pKa = 10.05NEE50 pKa = 4.52SEE52 pKa = 4.5YY53 pKa = 11.34NGTEE57 pKa = 3.93IIDD60 pKa = 3.75YY61 pKa = 9.55TLMQSTGLFDD71 pKa = 4.67KK72 pKa = 11.14NDD74 pKa = 3.37VEE76 pKa = 4.19IFEE79 pKa = 4.34WDD81 pKa = 3.19IVVNQYY87 pKa = 11.66GNVGYY92 pKa = 9.9IAYY95 pKa = 9.51LKK97 pKa = 10.29QEE99 pKa = 4.0AGFVVVLKK107 pKa = 10.86KK108 pKa = 10.15SDD110 pKa = 3.31YY111 pKa = 10.8RR112 pKa = 11.84LGHH115 pKa = 6.44RR116 pKa = 11.84NTGEE120 pKa = 4.2SYY122 pKa = 10.99DD123 pKa = 3.23ITNNHH128 pKa = 6.77KK129 pKa = 10.5IIGNIYY135 pKa = 9.32EE136 pKa = 4.37NPDD139 pKa = 3.35LLEE142 pKa = 4.41SVEE145 pKa = 4.25EE146 pKa = 4.18

Molecular weight: 16.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AA57|A0A4D6AA57_9CAUD Uncharacterized protein OS=Streptococcus phage Javan407 OX=2548153 GN=Javan407_0008 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 9.34FTVKK6 pKa = 10.32GSEE9 pKa = 3.99KK10 pKa = 10.53LEE12 pKa = 3.8EE13 pKa = 4.29RR14 pKa = 11.84LRR16 pKa = 11.84LMNSIRR22 pKa = 11.84WDD24 pKa = 3.55GVVNKK29 pKa = 10.52SLTQMFNRR37 pKa = 11.84GARR40 pKa = 11.84PPGTPVGKK48 pKa = 7.82QTKK51 pKa = 8.21NHH53 pKa = 6.19SAGEE57 pKa = 4.07LRR59 pKa = 11.84RR60 pKa = 11.84SRR62 pKa = 11.84RR63 pKa = 11.84KK64 pKa = 9.88QEE66 pKa = 3.54ISSKK70 pKa = 9.45NGSYY74 pKa = 9.67TGSFYY79 pKa = 9.74YY80 pKa = 10.33TKK82 pKa = 10.39DD83 pKa = 3.48YY84 pKa = 10.82APHH87 pKa = 6.47VEE89 pKa = 4.05YY90 pKa = 10.64GHH92 pKa = 6.97RR93 pKa = 11.84IVRR96 pKa = 11.84KK97 pKa = 8.66GKK99 pKa = 9.39QVGYY103 pKa = 11.16ANGKK107 pKa = 9.69KK108 pKa = 10.18YY109 pKa = 10.9LFNNVQKK116 pKa = 10.47QRR118 pKa = 11.84QIYY121 pKa = 8.74FKK123 pKa = 11.49DD124 pKa = 3.21MLAEE128 pKa = 4.05LTKK131 pKa = 10.68KK132 pKa = 10.43RR133 pKa = 3.9

Molecular weight: 15.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

11375

38

1398

180.6

20.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.171 ± 0.324

0.448 ± 0.069

6.198 ± 0.284

7.464 ± 0.587

4.176 ± 0.18

6.47 ± 0.512

1.31 ± 0.115

7.719 ± 0.273

9.116 ± 0.454

8.0 ± 0.341

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.382 ± 0.17

5.864 ± 0.231

2.76 ± 0.163

3.648 ± 0.253

3.446 ± 0.246

6.382 ± 0.453

6.585 ± 0.48

6.47 ± 0.263

1.284 ± 0.115

4.105 ± 0.298
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski