Amino acid dipepetide frequency for Clostridium sp. CAG:242

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.419AlaAla: 7.419 ± 0.136	1.302AlaCys: 1.302 ± 0.047	4.048AlaAsp: 4.048 ± 0.076	4.885AlaGlu: 4.885 ± 0.094	3.158AlaPhe: 3.158 ± 0.063	5.51AlaGly: 5.51 ± 0.113	1.149AlaHis: 1.149 ± 0.04	4.958AlaIle: 4.958 ± 0.096	4.871AlaLys: 4.871 ± 0.089	7.426AlaLeu: 7.426 ± 0.11	2.476AlaMet: 2.476 ± 0.066	2.626AlaAsn: 2.626 ± 0.07	2.168AlaPro: 2.168 ± 0.053	3.748AlaGln: 3.748 ± 0.085	2.841AlaArg: 2.841 ± 0.066	4.302AlaSer: 4.302 ± 0.086	3.065AlaThr: 3.065 ± 0.084	6.401AlaVal: 6.401 ± 0.125	0.569AlaTrp: 0.569 ± 0.031	2.333AlaTyr: 2.333 ± 0.057	0.01AlaXaa: 0.01 ± 0.004
Cys	1.297CysAla: 1.297 ± 0.043	0.448CysCys: 0.448 ± 0.029	1.054CysAsp: 1.054 ± 0.04	1.061CysGlu: 1.061 ± 0.041	0.817CysPhe: 0.817 ± 0.043	1.724CysGly: 1.724 ± 0.051	0.401CysHis: 0.401 ± 0.025	1.141CysIle: 1.141 ± 0.046	0.906CysLys: 0.906 ± 0.035	1.445CysLeu: 1.445 ± 0.052	0.517CysMet: 0.517 ± 0.028	0.6CysAsn: 0.6 ± 0.033	0.814CysPro: 0.814 ± 0.04	0.594CysGln: 0.594 ± 0.031	0.851CysArg: 0.851 ± 0.033	1.193CysSer: 1.193 ± 0.037	0.89CysThr: 0.89 ± 0.039	1.12CysVal: 1.12 ± 0.043	0.175CysTrp: 0.175 ± 0.015	0.57CysTyr: 0.57 ± 0.03	0.0CysXaa: 0.0 ± 0.0
Asp	3.864AspAla: 3.864 ± 0.084	0.992AspCys: 0.992 ± 0.04	2.681AspAsp: 2.681 ± 0.074	4.148AspGlu: 4.148 ± 0.082	2.706AspPhe: 2.706 ± 0.065	4.227AspGly: 4.227 ± 0.089	0.996AspHis: 0.996 ± 0.044	3.957AspIle: 3.957 ± 0.08	3.085AspLys: 3.085 ± 0.071	4.738AspLeu: 4.738 ± 0.083	1.461AspMet: 1.461 ± 0.042	1.955AspAsn: 1.955 ± 0.056	2.192AspPro: 2.192 ± 0.059	1.747AspGln: 1.747 ± 0.049	2.478AspArg: 2.478 ± 0.06	3.261AspSer: 3.261 ± 0.082	2.955AspThr: 2.955 ± 0.069	3.619AspVal: 3.619 ± 0.079	0.549AspTrp: 0.549 ± 0.029	2.5AspTyr: 2.5 ± 0.068	0.001AspXaa: 0.001 ± 0.001
Glu	4.605GluAla: 4.605 ± 0.09	0.862GluCys: 0.862 ± 0.032	4.01GluAsp: 4.01 ± 0.076	7.334GluGlu: 7.334 ± 0.138	2.399GluPhe: 2.399 ± 0.065	4.274GluGly: 4.274 ± 0.082	1.427GluHis: 1.427 ± 0.048	5.054GluIle: 5.054 ± 0.084	5.871GluLys: 5.871 ± 0.121	6.464GluLeu: 6.464 ± 0.104	2.435GluMet: 2.435 ± 0.058	3.948GluAsn: 3.948 ± 0.092	1.954GluPro: 1.954 ± 0.055	4.041GluGln: 4.041 ± 0.096	3.124GluArg: 3.124 ± 0.077	3.53GluSer: 3.53 ± 0.085	3.543GluThr: 3.543 ± 0.085	4.274GluVal: 4.274 ± 0.074	0.632GluTrp: 0.632 ± 0.031	2.817GluTyr: 2.817 ± 0.069	0.0GluXaa: 0.0 ± 0.0
Phe	3.378PheAla: 3.378 ± 0.074	1.071PheCys: 1.071 ± 0.044	2.603PheAsp: 2.603 ± 0.062	2.661PheGlu: 2.661 ± 0.069	2.173PhePhe: 2.173 ± 0.069	3.19PheGly: 3.19 ± 0.077	0.927PheHis: 0.927 ± 0.038	2.402PheIle: 2.402 ± 0.063	1.517PheLys: 1.517 ± 0.05	4.636PheLeu: 4.636 ± 0.101	1.034PheMet: 1.034 ± 0.038	1.451PheAsn: 1.451 ± 0.047	1.544PhePro: 1.544 ± 0.047	1.418PheGln: 1.418 ± 0.04	1.583PheArg: 1.583 ± 0.043	3.626PheSer: 3.626 ± 0.077	2.402PheThr: 2.402 ± 0.063	3.092PheVal: 3.092 ± 0.072	0.483PheTrp: 0.483 ± 0.025	1.775PheTyr: 1.775 ± 0.051	0.0PheXaa: 0.0 ± 0.0
Gly	4.905GlyAla: 4.905 ± 0.105	1.576GlyCys: 1.576 ± 0.057	3.336GlyAsp: 3.336 ± 0.068	5.057GlyGlu: 5.057 ± 0.08	3.186GlyPhe: 3.186 ± 0.072	4.627GlyGly: 4.627 ± 0.1	1.204GlyHis: 1.204 ± 0.044	5.512GlyIle: 5.512 ± 0.102	5.13GlyLys: 5.13 ± 0.097	5.853GlyLeu: 5.853 ± 0.116	2.296GlyMet: 2.296 ± 0.064	2.861GlyAsn: 2.861 ± 0.077	1.328GlyPro: 1.328 ± 0.047	2.383GlyGln: 2.383 ± 0.061	2.913GlyArg: 2.913 ± 0.07	4.168GlySer: 4.168 ± 0.095	3.767GlyThr: 3.767 ± 0.082	5.13GlyVal: 5.13 ± 0.087	0.725GlyTrp: 0.725 ± 0.033	2.943GlyTyr: 2.943 ± 0.068	0.003GlyXaa: 0.003 ± 0.002
His	1.228HisAla: 1.228 ± 0.046	0.396HisCys: 0.396 ± 0.025	0.973HisAsp: 0.973 ± 0.042	1.056HisGlu: 1.056 ± 0.042	0.948HisPhe: 0.948 ± 0.035	1.279HisGly: 1.279 ± 0.051	0.508HisHis: 0.508 ± 0.034	1.289HisIle: 1.289 ± 0.046	0.987HisLys: 0.987 ± 0.034	1.831HisLeu: 1.831 ± 0.054	0.376HisMet: 0.376 ± 0.022	0.748HisAsn: 0.748 ± 0.034	1.099HisPro: 1.099 ± 0.038	0.749HisGln: 0.749 ± 0.032	0.871HisArg: 0.871 ± 0.034	1.071HisSer: 1.071 ± 0.042	1.004HisThr: 1.004 ± 0.041	1.007HisVal: 1.007 ± 0.034	0.206HisTrp: 0.206 ± 0.019	0.748HisTyr: 0.748 ± 0.028	0.001HisXaa: 0.001 ± 0.001
Ile	5.772IleAla: 5.772 ± 0.11	1.486IleCys: 1.486 ± 0.04	4.037IleAsp: 4.037 ± 0.083	4.488IleGlu: 4.488 ± 0.087	2.966IlePhe: 2.966 ± 0.077	4.851IleGly: 4.851 ± 0.106	1.323IleHis: 1.323 ± 0.045	4.358IleIle: 4.358 ± 0.104	3.248IleLys: 3.248 ± 0.085	7.339IleLeu: 7.339 ± 0.124	1.686IleMet: 1.686 ± 0.05	2.65IleAsn: 2.65 ± 0.064	3.165IlePro: 3.165 ± 0.074	2.479IleGln: 2.479 ± 0.063	3.334IleArg: 3.334 ± 0.067	5.065IleSer: 5.065 ± 0.097	3.927IleThr: 3.927 ± 0.079	4.64IleVal: 4.64 ± 0.097	0.603IleTrp: 0.603 ± 0.031	2.252IleTyr: 2.252 ± 0.053	0.0IleXaa: 0.0 ± 0.0
Lys	4.575LysAla: 4.575 ± 0.088	0.606LysCys: 0.606 ± 0.028	3.15LysAsp: 3.15 ± 0.068	5.746LysGlu: 5.746 ± 0.119	1.641LysPhe: 1.641 ± 0.04	3.707LysGly: 3.707 ± 0.089	0.958LysHis: 0.958 ± 0.037	4.45LysIle: 4.45 ± 0.082	4.95LysLys: 4.95 ± 0.093	5.315LysLeu: 5.315 ± 0.096	2.226LysMet: 2.226 ± 0.063	3.058LysAsn: 3.058 ± 0.081	2.071LysPro: 2.071 ± 0.057	2.635LysGln: 2.635 ± 0.063	2.871LysArg: 2.871 ± 0.068	2.9LysSer: 2.9 ± 0.067	3.616LysThr: 3.616 ± 0.074	3.986LysVal: 3.986 ± 0.084	0.459LysTrp: 0.459 ± 0.024	2.048LysTyr: 2.048 ± 0.058	0.0LysXaa: 0.0 ± 0.0
Leu	7.046LeuAla: 7.046 ± 0.112	2.216LeuCys: 2.216 ± 0.073	5.196LeuAsp: 5.196 ± 0.09	6.388LeuGlu: 6.388 ± 0.098	4.476LeuPhe: 4.476 ± 0.111	6.472LeuGly: 6.472 ± 0.105	1.804LeuHis: 1.804 ± 0.055	6.365LeuIle: 6.365 ± 0.121	5.462LeuLys: 5.462 ± 0.088	9.345LeuLeu: 9.345 ± 0.173	2.8LeuMet: 2.8 ± 0.071	3.647LeuAsn: 3.647 ± 0.068	4.178LeuPro: 4.178 ± 0.088	3.151LeuGln: 3.151 ± 0.069	4.012LeuArg: 4.012 ± 0.077	7.918LeuSer: 7.918 ± 0.106	5.378LeuThr: 5.378 ± 0.089	5.585LeuVal: 5.585 ± 0.098	0.832LeuTrp: 0.832 ± 0.032	3.288LeuTyr: 3.288 ± 0.08	0.004LeuXaa: 0.004 ± 0.003
Met	2.242MetAla: 2.242 ± 0.054	0.318MetCys: 0.318 ± 0.019	1.617MetAsp: 1.617 ± 0.046	2.311MetGlu: 2.311 ± 0.061	0.982MetPhe: 0.982 ± 0.042	2.233MetGly: 2.233 ± 0.061	0.435MetHis: 0.435 ± 0.025	2.254MetIle: 2.254 ± 0.061	2.251MetLys: 2.251 ± 0.048	3.013MetLeu: 3.013 ± 0.068	1.123MetMet: 1.123 ± 0.043	1.335MetAsn: 1.335 ± 0.043	1.165MetPro: 1.165 ± 0.038	1.365MetGln: 1.365 ± 0.053	1.233MetArg: 1.233 ± 0.04	1.786MetSer: 1.786 ± 0.048	1.704MetThr: 1.704 ± 0.046	2.123MetVal: 2.123 ± 0.055	0.213MetTrp: 0.213 ± 0.018	0.801MetTyr: 0.801 ± 0.038	0.001MetXaa: 0.001 ± 0.001
Asn	2.954AsnAla: 2.954 ± 0.071	0.718AsnCys: 0.718 ± 0.036	1.911AsnAsp: 1.911 ± 0.053	2.385AsnGlu: 2.385 ± 0.06	1.642AsnPhe: 1.642 ± 0.045	3.192AsnGly: 3.192 ± 0.091	0.894AsnHis: 0.894 ± 0.04	3.148AsnIle: 3.148 ± 0.068	2.064AsnLys: 2.064 ± 0.052	4.006AsnLeu: 4.006 ± 0.1	1.147AsnMet: 1.147 ± 0.042	1.647AsnAsn: 1.647 ± 0.055	2.221AsnPro: 2.221 ± 0.066	1.702AsnGln: 1.702 ± 0.05	1.92AsnArg: 1.92 ± 0.049	2.18AsnSer: 2.18 ± 0.071	2.31AsnThr: 2.31 ± 0.06	2.478AsnVal: 2.478 ± 0.068	0.393AsnTrp: 0.393 ± 0.026	1.586AsnTyr: 1.586 ± 0.051	0.0AsnXaa: 0.0 ± 0.0
Pro	2.635ProAla: 2.635 ± 0.063	0.545ProCys: 0.545 ± 0.033	2.504ProAsp: 2.504 ± 0.056	3.219ProGlu: 3.219 ± 0.077	1.735ProPhe: 1.735 ± 0.054	2.097ProGly: 2.097 ± 0.061	0.637ProHis: 0.637 ± 0.03	2.392ProIle: 2.392 ± 0.061	2.21ProLys: 2.21 ± 0.059	3.134ProLeu: 3.134 ± 0.063	1.058ProMet: 1.058 ± 0.039	1.435ProAsn: 1.435 ± 0.046	0.909ProPro: 0.909 ± 0.038	1.892ProGln: 1.892 ± 0.051	1.016ProArg: 1.016 ± 0.041	2.33ProSer: 2.33 ± 0.067	1.809ProThr: 1.809 ± 0.058	3.252ProVal: 3.252 ± 0.061	0.293ProTrp: 0.293 ± 0.021	1.382ProTyr: 1.382 ± 0.041	0.001ProXaa: 0.001 ± 0.001
Gln	3.206GlnAla: 3.206 ± 0.071	0.397GlnCys: 0.397 ± 0.023	1.976GlnAsp: 1.976 ± 0.056	3.209GlnGlu: 3.209 ± 0.079	1.31GlnPhe: 1.31 ± 0.047	2.288GlnGly: 2.288 ± 0.056	0.642GlnHis: 0.642 ± 0.031	3.148GlnIle: 3.148 ± 0.065	3.331GlnLys: 3.331 ± 0.079	3.717GlnLeu: 3.717 ± 0.08	1.531GlnMet: 1.531 ± 0.052	2.295GlnAsn: 2.295 ± 0.067	1.362GlnPro: 1.362 ± 0.046	2.3GlnGln: 2.3 ± 0.076	1.806GlnArg: 1.806 ± 0.048	2.335GlnSer: 2.335 ± 0.066	2.483GlnThr: 2.483 ± 0.064	2.493GlnVal: 2.493 ± 0.057	0.352GlnTrp: 0.352 ± 0.023	1.396GlnTyr: 1.396 ± 0.044	0.001GlnXaa: 0.001 ± 0.002
Arg	2.795ArgAla: 2.795 ± 0.064	0.778ArgCys: 0.778 ± 0.036	2.093ArgAsp: 2.093 ± 0.057	3.658ArgGlu: 3.658 ± 0.079	2.03ArgPhe: 2.03 ± 0.05	2.372ArgGly: 2.372 ± 0.062	0.796ArgHis: 0.796 ± 0.033	3.224ArgIle: 3.224 ± 0.071	3.048ArgLys: 3.048 ± 0.069	3.969ArgLeu: 3.969 ± 0.082	1.668ArgMet: 1.668 ± 0.047	1.693ArgAsn: 1.693 ± 0.053	1.403ArgPro: 1.403 ± 0.043	1.911ArgGln: 1.911 ± 0.053	2.226ArgArg: 2.226 ± 0.074	2.244ArgSer: 2.244 ± 0.059	2.133ArgThr: 2.133 ± 0.053	2.776ArgVal: 2.776 ± 0.06	0.427ArgTrp: 0.427 ± 0.026	1.887ArgTyr: 1.887 ± 0.053	0.0ArgXaa: 0.0 ± 0.0
Ser	4.869SerAla: 4.869 ± 0.095	1.096SerCys: 1.096 ± 0.044	3.481SerAsp: 3.481 ± 0.08	3.929SerGlu: 3.929 ± 0.082	3.271SerPhe: 3.271 ± 0.07	5.068SerGly: 5.068 ± 0.094	1.145SerHis: 1.145 ± 0.041	4.496SerIle: 4.496 ± 0.101	3.238SerLys: 3.238 ± 0.076	6.075SerLeu: 6.075 ± 0.106	2.042SerMet: 2.042 ± 0.059	2.192SerAsn: 2.192 ± 0.061	2.124SerPro: 2.124 ± 0.061	2.75SerGln: 2.75 ± 0.068	2.758SerArg: 2.758 ± 0.069	4.42SerSer: 4.42 ± 0.118	3.038SerThr: 3.038 ± 0.083	4.64SerVal: 4.64 ± 0.089	0.634SerTrp: 0.634 ± 0.03	2.309SerTyr: 2.309 ± 0.06	0.001SerXaa: 0.001 ± 0.001
Thr	4.589ThrAla: 4.589 ± 0.1	0.687ThrCys: 0.687 ± 0.031	2.871ThrAsp: 2.871 ± 0.066	3.69ThrGlu: 3.69 ± 0.094	2.279ThrPhe: 2.279 ± 0.059	3.979ThrGly: 3.979 ± 0.08	0.91ThrHis: 0.91 ± 0.034	3.926ThrIle: 3.926 ± 0.084	2.672ThrLys: 2.672 ± 0.065	5.362ThrLeu: 5.362 ± 0.095	1.465ThrMet: 1.465 ± 0.047	1.872ThrAsn: 1.872 ± 0.054	2.427ThrPro: 2.427 ± 0.061	2.069ThrGln: 2.069 ± 0.054	1.947ThrArg: 1.947 ± 0.052	3.297ThrSer: 3.297 ± 0.084	2.975ThrThr: 2.975 ± 0.087	4.416ThrVal: 4.416 ± 0.093	0.394ThrTrp: 0.394 ± 0.022	1.733ThrTyr: 1.733 ± 0.064	0.001ThrXaa: 0.001 ± 0.001
Val	4.796ValAla: 4.796 ± 0.097	1.361ValCys: 1.361 ± 0.049	3.778ValAsp: 3.778 ± 0.084	4.338ValGlu: 4.338 ± 0.082	3.121ValPhe: 3.121 ± 0.076	4.54ValGly: 4.54 ± 0.105	1.116ValHis: 1.116 ± 0.04	4.709ValIle: 4.709 ± 0.081	3.914ValLys: 3.914 ± 0.077	7.161ValLeu: 7.161 ± 0.109	2.059ValMet: 2.059 ± 0.063	2.503ValAsn: 2.503 ± 0.06	2.569ValPro: 2.569 ± 0.049	2.368ValGln: 2.368 ± 0.055	3.095ValArg: 3.095 ± 0.071	5.154ValSer: 5.154 ± 0.104	3.993ValThr: 3.993 ± 0.092	5.192ValVal: 5.192 ± 0.094	0.67ValTrp: 0.67 ± 0.028	2.404ValTyr: 2.404 ± 0.058	0.001ValXaa: 0.001 ± 0.002
Trp	0.587TrpAla: 0.587 ± 0.026	0.168TrpCys: 0.168 ± 0.014	0.521TrpAsp: 0.521 ± 0.031	0.709TrpGlu: 0.709 ± 0.033	0.447TrpPhe: 0.447 ± 0.026	0.697TrpGly: 0.697 ± 0.032	0.183TrpHis: 0.183 ± 0.017	0.67TrpIle: 0.67 ± 0.033	0.672TrpLys: 0.672 ± 0.029	0.847TrpLeu: 0.847 ± 0.034	0.351TrpMet: 0.351 ± 0.024	0.466TrpAsn: 0.466 ± 0.024	0.163TrpPro: 0.163 ± 0.016	0.417TrpGln: 0.417 ± 0.024	0.32TrpArg: 0.32 ± 0.023	0.459TrpSer: 0.459 ± 0.024	0.4TrpThr: 0.4 ± 0.024	0.573TrpVal: 0.573 ± 0.033	0.077TrpTrp: 0.077 ± 0.011	0.345TrpTyr: 0.345 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.496TyrAla: 2.496 ± 0.064	0.594TyrCys: 0.594 ± 0.03	2.271TyrAsp: 2.271 ± 0.053	2.327TyrGlu: 2.327 ± 0.062	1.675TyrPhe: 1.675 ± 0.049	2.568TyrGly: 2.568 ± 0.056	0.92TyrHis: 0.92 ± 0.035	2.217TyrIle: 2.217 ± 0.057	1.537TyrLys: 1.537 ± 0.058	3.888TyrLeu: 3.888 ± 0.071	0.754TyrMet: 0.754 ± 0.032	1.517TyrAsn: 1.517 ± 0.043	1.63TyrPro: 1.63 ± 0.049	1.859TyrGln: 1.859 ± 0.056	1.93TyrArg: 1.93 ± 0.054	2.334TyrSer: 2.334 ± 0.062	2.171TyrThr: 2.171 ± 0.063	2.047TyrVal: 2.047 ± 0.049	0.385TyrTrp: 0.385 ± 0.026	1.537TyrTyr: 1.537 ± 0.052	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.006XaaAla: 0.006 ± 0.002	0.003XaaCys: 0.003 ± 0.002	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.002	0.006XaaGly: 0.006 ± 0.003	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.007XaaArg: 0.007 ± 0.003	0.003XaaSer: 0.003 ± 0.002	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.002	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.028XaaXaa: 0.028 ± 0.009

Statistics based on 2433 proteins (709936 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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