Avon-Heathcote Estuary associated circular virus 28

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IMN2|A0A0C5IMN2_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 28 OX=1618252 PE=4 SV=1
MM1 pKa = 7.52ARR3 pKa = 11.84THH5 pKa = 6.42AKK7 pKa = 10.35KK8 pKa = 8.92NTSKK12 pKa = 11.0DD13 pKa = 3.18PSFRR17 pKa = 11.84KK18 pKa = 8.05TKK20 pKa = 9.43PLEE23 pKa = 3.86YY24 pKa = 10.54LPVQRR29 pKa = 11.84KK30 pKa = 6.77ITLGTVSGATTYY42 pKa = 11.63GLIDD46 pKa = 3.92AGRR49 pKa = 11.84LLSTSNHH56 pKa = 5.08RR57 pKa = 11.84LYY59 pKa = 10.63RR60 pKa = 11.84YY61 pKa = 8.81GKK63 pKa = 10.03RR64 pKa = 11.84YY65 pKa = 8.92NLKK68 pKa = 9.73IDD70 pKa = 3.64IDD72 pKa = 4.04PSVAATNTIEE82 pKa = 3.88VWALADD88 pKa = 3.21TWAVQKK94 pKa = 10.95AFEE97 pKa = 3.92EE98 pKa = 4.33AKK100 pKa = 10.44RR101 pKa = 11.84VFDD104 pKa = 3.95KK105 pKa = 11.15AYY107 pKa = 8.58TNEE110 pKa = 4.05RR111 pKa = 11.84EE112 pKa = 4.21NLSKK116 pKa = 10.71DD117 pKa = 3.01ARR119 pKa = 11.84ARR121 pKa = 11.84WFDD124 pKa = 3.36FRR126 pKa = 11.84ASIGVSGDD134 pKa = 2.46IMMAAVSTDD143 pKa = 3.89PNPGTLTLLTGGEE156 pKa = 4.2FANSIVEE163 pKa = 4.3DD164 pKa = 3.58NAGVQRR170 pKa = 11.84AFSWSPATSASTYY183 pKa = 10.16SIPAEE188 pKa = 3.8YY189 pKa = 10.56DD190 pKa = 3.22LAGNTSTSPTTPTGAGPYY208 pKa = 10.15DD209 pKa = 4.26DD210 pKa = 5.57LQADD214 pKa = 3.99SSAIEE219 pKa = 4.06MEE221 pKa = 4.11ALQDD225 pKa = 3.7RR226 pKa = 11.84GNLPPYY232 pKa = 9.0EE233 pKa = 4.92ANTFPPVWVKK243 pKa = 10.43IATLGQSAAGSQRR256 pKa = 11.84LSTGFFDD263 pKa = 4.53APCGQVVLSSSAPITEE279 pKa = 4.45LTGKK283 pKa = 10.34AYY285 pKa = 10.42AEE287 pKa = 4.21FKK289 pKa = 10.54IGDD292 pKa = 3.76YY293 pKa = 10.81KK294 pKa = 10.88GVKK297 pKa = 8.97AHH299 pKa = 6.04NLEE302 pKa = 4.26RR303 pKa = 11.84MM304 pKa = 3.65

Molecular weight: 32.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IMN2|A0A0C5IMN2_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 28 OX=1618252 PE=4 SV=1
MM1 pKa = 6.99LQEE4 pKa = 4.65SIVRR8 pKa = 11.84TPRR11 pKa = 11.84FPPNVCWGVSGKK23 pKa = 9.44WEE25 pKa = 4.1DD26 pKa = 3.29QCMKK30 pKa = 10.66VEE32 pKa = 4.06ATPDD36 pKa = 3.0GCLNARR42 pKa = 11.84QFLNDD47 pKa = 3.48RR48 pKa = 11.84SLCPRR53 pKa = 11.84WRR55 pKa = 11.84LCPGCEE61 pKa = 3.58KK62 pKa = 10.51VRR64 pKa = 11.84AKK66 pKa = 10.55RR67 pKa = 11.84NQYY70 pKa = 10.39KK71 pKa = 9.4IAKK74 pKa = 9.03RR75 pKa = 11.84LEE77 pKa = 4.2FDD79 pKa = 4.64LEE81 pKa = 4.12WAKK84 pKa = 10.78EE85 pKa = 3.54ADD87 pKa = 3.4IPLKK91 pKa = 10.97VGVLTTTLPGKK102 pKa = 9.81EE103 pKa = 3.59SWIRR107 pKa = 11.84NASLGEE113 pKa = 3.93QYY115 pKa = 11.19SYY117 pKa = 10.12LTEE120 pKa = 3.97RR121 pKa = 11.84RR122 pKa = 11.84TMSGYY127 pKa = 9.08TGWHH131 pKa = 5.56SMRR134 pKa = 11.84GLNTKK139 pKa = 10.09LKK141 pKa = 9.52EE142 pKa = 3.84WGISGGSHH150 pKa = 5.16YY151 pKa = 11.26LEE153 pKa = 4.47FTNKK157 pKa = 7.65GTTWNTHH164 pKa = 3.61MHH166 pKa = 6.49SILVGFQDD174 pKa = 4.04DD175 pKa = 3.71WQVPLKK181 pKa = 9.47EE182 pKa = 4.34TTKK185 pKa = 10.33QLEE188 pKa = 4.41WNDD191 pKa = 3.92DD192 pKa = 3.1LTMRR196 pKa = 11.84LQTEE200 pKa = 3.82KK201 pKa = 11.24AEE203 pKa = 4.15NKK205 pKa = 8.3TRR207 pKa = 11.84SNKK210 pKa = 10.14RR211 pKa = 11.84ILEE214 pKa = 3.92PLGLGRR220 pKa = 11.84LYY222 pKa = 10.6TLDD225 pKa = 3.36IASDD229 pKa = 4.05DD230 pKa = 3.91EE231 pKa = 4.3LASIARR237 pKa = 11.84YY238 pKa = 7.54SAKK241 pKa = 10.16VEE243 pKa = 4.26YY244 pKa = 8.82VTKK247 pKa = 9.96PVKK250 pKa = 10.62VPEE253 pKa = 4.55GKK255 pKa = 10.5LVDD258 pKa = 3.74VTNFLAGGFEE268 pKa = 4.23HH269 pKa = 6.79GKK271 pKa = 9.37EE272 pKa = 3.88RR273 pKa = 11.84SGHH276 pKa = 5.01GRR278 pKa = 11.84HH279 pKa = 6.05LPRR282 pKa = 11.84LARR285 pKa = 11.84PFGDD289 pKa = 2.81WMKK292 pKa = 10.82NGPEE296 pKa = 3.43RR297 pKa = 11.84RR298 pKa = 11.84YY299 pKa = 10.62AA300 pKa = 3.48

Molecular weight: 34.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

604

300

304

302.0

33.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.609 ± 2.535

1.159 ± 0.591

5.298 ± 0.443

6.291 ± 0.966

3.146 ± 0.336

7.781 ± 0.388

1.656 ± 0.476

3.642 ± 0.451

6.623 ± 0.733

8.609 ± 0.977

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.987 ± 0.243

4.305 ± 0.02

5.298 ± 0.209

2.815 ± 0.13

6.623 ± 0.968

6.954 ± 1.138

8.113 ± 0.782

4.967 ± 0.023

2.649 ± 0.715

3.477 ± 0.335
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski