Sanxia water strider virus 14

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGJ6|A0A1L3KGJ6_9VIRU RNA-directed RNA polymerase OS=Sanxia water strider virus 14 OX=1923398 PE=4 SV=1
MM1 pKa = 7.69PKK3 pKa = 10.33SKK5 pKa = 10.05GHH7 pKa = 6.27SLLPNCSHH15 pKa = 7.29RR16 pKa = 11.84AKK18 pKa = 10.25TSDD21 pKa = 3.75CRR23 pKa = 11.84CSRR26 pKa = 11.84ATYY29 pKa = 10.94KK30 pKa = 10.96LFDD33 pKa = 3.78YY34 pKa = 10.92SLEE37 pKa = 4.07HH38 pKa = 7.11LNEE41 pKa = 4.29SVVWTHH47 pKa = 6.97RR48 pKa = 11.84GCVCNEE54 pKa = 3.62LVALRR59 pKa = 11.84EE60 pKa = 3.94RR61 pKa = 11.84HH62 pKa = 5.55QLDD65 pKa = 2.92TGARR69 pKa = 11.84YY70 pKa = 8.9SAPINAVSVNVDD82 pKa = 3.61EE83 pKa = 6.05LVMANAGRR91 pKa = 11.84FPEE94 pKa = 4.39LKK96 pKa = 9.92RR97 pKa = 11.84NAFSVVISHH106 pKa = 6.03YY107 pKa = 10.84SGGKK111 pKa = 7.33RR112 pKa = 11.84AEE114 pKa = 3.98YY115 pKa = 10.19EE116 pKa = 3.88RR117 pKa = 11.84ARR119 pKa = 11.84EE120 pKa = 3.98SLLIDD125 pKa = 3.75PLVEE129 pKa = 4.1RR130 pKa = 11.84DD131 pKa = 3.0SRR133 pKa = 11.84LRR135 pKa = 11.84MFLKK139 pKa = 10.36DD140 pKa = 4.09DD141 pKa = 5.01KK142 pKa = 11.17YY143 pKa = 10.68HH144 pKa = 5.67TWDD147 pKa = 4.47FKK149 pKa = 11.37APRR152 pKa = 11.84CIQFRR157 pKa = 11.84SKK159 pKa = 10.76RR160 pKa = 11.84YY161 pKa = 9.24GLTLSSYY168 pKa = 8.91LQPIEE173 pKa = 4.04EE174 pKa = 4.25FTYY177 pKa = 10.4EE178 pKa = 4.88LRR180 pKa = 11.84DD181 pKa = 3.66TTDD184 pKa = 2.46SHH186 pKa = 7.71VFAKK190 pKa = 10.37SRR192 pKa = 11.84NLDD195 pKa = 3.25DD196 pKa = 4.07RR197 pKa = 11.84AADD200 pKa = 5.32LRR202 pKa = 11.84LKK204 pKa = 9.51WDD206 pKa = 3.6SFIDD210 pKa = 4.51PIAYY214 pKa = 10.24CLDD217 pKa = 3.32HH218 pKa = 7.19SKK220 pKa = 10.62FDD222 pKa = 3.53CHH224 pKa = 7.73VNVDD228 pKa = 4.23LLKK231 pKa = 10.48QEE233 pKa = 3.73HH234 pKa = 6.52RR235 pKa = 11.84YY236 pKa = 9.35YY237 pKa = 10.54RR238 pKa = 11.84QFYY241 pKa = 9.65PRR243 pKa = 11.84DD244 pKa = 3.28AGLRR248 pKa = 11.84KK249 pKa = 9.67LLSQQINNRR258 pKa = 11.84GSTKK262 pKa = 10.77NGTTYY267 pKa = 8.78KK268 pKa = 9.6TCGTRR273 pKa = 11.84MSGDD277 pKa = 3.73PNTGLGNSILNYY289 pKa = 10.48GMLRR293 pKa = 11.84EE294 pKa = 4.11AFKK297 pKa = 10.69KK298 pKa = 10.29VRR300 pKa = 11.84AAYY303 pKa = 10.3YY304 pKa = 9.78IDD306 pKa = 5.19GDD308 pKa = 3.96DD309 pKa = 3.39SVVIIEE315 pKa = 5.04SADD318 pKa = 3.46QVNVNVGCFEE328 pKa = 4.23RR329 pKa = 11.84FGMKK333 pKa = 9.6TKK335 pKa = 10.82VEE337 pKa = 3.93VATIFEE343 pKa = 4.39HH344 pKa = 7.35TEE346 pKa = 3.87FCQCRR351 pKa = 11.84PVYY354 pKa = 10.59DD355 pKa = 3.57GLKK358 pKa = 7.98WHH360 pKa = 6.97LVRR363 pKa = 11.84NPYY366 pKa = 8.39RR367 pKa = 11.84TLARR371 pKa = 11.84LPWIVKK377 pKa = 9.88KK378 pKa = 10.64NHH380 pKa = 6.39LSCKK384 pKa = 9.52ARR386 pKa = 11.84YY387 pKa = 8.13IKK389 pKa = 10.71SVGLCEE395 pKa = 4.24LALNMGIPVLQQIASLMVEE414 pKa = 4.05RR415 pKa = 11.84GEE417 pKa = 4.19GKK419 pKa = 10.16YY420 pKa = 10.38IKK422 pKa = 9.52TDD424 pKa = 2.89RR425 pKa = 11.84HH426 pKa = 5.47FMAKK430 pKa = 9.95RR431 pKa = 11.84ATIKK435 pKa = 9.6PWHH438 pKa = 6.21ARR440 pKa = 11.84KK441 pKa = 10.07VPIRR445 pKa = 11.84EE446 pKa = 3.88VTRR449 pKa = 11.84EE450 pKa = 3.94SFEE453 pKa = 4.16IAWGISRR460 pKa = 11.84EE461 pKa = 4.06EE462 pKa = 3.78QLEE465 pKa = 4.31LEE467 pKa = 4.53NVTLSLPHH475 pKa = 6.94GDD477 pKa = 4.09AEE479 pKa = 4.8SLFYY483 pKa = 10.35THH485 pKa = 7.62LAPGVTHH492 pKa = 6.6CEE494 pKa = 4.24CC495 pKa = 5.1

Molecular weight: 57.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG71|A0A1L3KG71_9VIRU Uncharacterized protein OS=Sanxia water strider virus 14 OX=1923398 PE=4 SV=1
MM1 pKa = 6.44QTSTSYY7 pKa = 11.23KK8 pKa = 9.62PDD10 pKa = 3.12LKK12 pKa = 11.1KK13 pKa = 10.91FGEE16 pKa = 4.15QRR18 pKa = 11.84ARR20 pKa = 11.84TSWQGPKK27 pKa = 10.04SSQNGFRR34 pKa = 11.84RR35 pKa = 11.84TPTGQGSPAANKK47 pKa = 7.32QQCSSCGEE55 pKa = 3.78LGKK58 pKa = 10.4RR59 pKa = 11.84LRR61 pKa = 11.84KK62 pKa = 9.77LEE64 pKa = 3.79EE65 pKa = 4.43RR66 pKa = 11.84IRR68 pKa = 11.84TLSNKK73 pKa = 9.26HH74 pKa = 5.03VRR76 pKa = 11.84LPSATGSWQTVCRR89 pKa = 11.84EE90 pKa = 3.59KK91 pKa = 10.83VAPKK95 pKa = 10.05SRR97 pKa = 11.84QRR99 pKa = 11.84PTTSHH104 pKa = 6.06QDD106 pKa = 3.11RR107 pKa = 11.84TGVKK111 pKa = 9.49PLNNQEE117 pKa = 3.84RR118 pKa = 11.84DD119 pKa = 3.56TILRR123 pKa = 11.84KK124 pKa = 9.45HH125 pKa = 6.69DD126 pKa = 4.26LLNPSSTNQARR137 pKa = 11.84RR138 pKa = 11.84VGSVPNVSSAGTSGEE153 pKa = 4.31SMTPLLKK160 pKa = 9.69TASLSRR166 pKa = 11.84ILPSRR171 pKa = 11.84RR172 pKa = 11.84TSPRR176 pKa = 11.84RR177 pKa = 11.84SSRR180 pKa = 11.84RR181 pKa = 11.84SDD183 pKa = 2.74ATTSAKK189 pKa = 10.24KK190 pKa = 10.65SSGPDD195 pKa = 3.29AACATAPRR203 pKa = 11.84TAKK206 pKa = 10.37AAAATVQSGSVIEE219 pKa = 4.79ALATTPKK226 pKa = 10.31DD227 pKa = 3.49LKK229 pKa = 10.59RR230 pKa = 11.84RR231 pKa = 11.84PGYY234 pKa = 10.46KK235 pKa = 9.02SDD237 pKa = 3.96RR238 pKa = 11.84DD239 pKa = 3.62SPTIKK244 pKa = 10.18RR245 pKa = 11.84VTWEE249 pKa = 3.66ARR251 pKa = 11.84RR252 pKa = 11.84APDD255 pKa = 3.79DD256 pKa = 4.4AADD259 pKa = 3.76KK260 pKa = 10.49PVYY263 pKa = 9.08ATNTSKK269 pKa = 10.84RR270 pKa = 11.84GPARR274 pKa = 11.84KK275 pKa = 9.5VPLGEE280 pKa = 4.01RR281 pKa = 11.84RR282 pKa = 11.84RR283 pKa = 11.84QRR285 pKa = 11.84KK286 pKa = 9.17AFVDD290 pKa = 3.59VDD292 pKa = 4.46DD293 pKa = 4.08NLYY296 pKa = 9.89WYY298 pKa = 10.52LVLEE302 pKa = 4.68FALVPRR308 pKa = 11.84TSDD311 pKa = 2.79ILRR314 pKa = 11.84QMKK317 pKa = 10.33AKK319 pKa = 10.42AKK321 pKa = 10.28QFLNLHH327 pKa = 6.46DD328 pKa = 5.64LNDD331 pKa = 3.62ISLKK335 pKa = 10.57DD336 pKa = 3.53QYY338 pKa = 11.46TMVIGTISRR347 pKa = 11.84AMLVPEE353 pKa = 4.19QEE355 pKa = 3.79MKK357 pKa = 10.64LRR359 pKa = 11.84QEE361 pKa = 4.59LKK363 pKa = 9.63SDD365 pKa = 3.45AAIEE369 pKa = 4.09EE370 pKa = 4.42MGKK373 pKa = 9.14HH374 pKa = 5.36AKK376 pKa = 9.16LVKK379 pKa = 10.02RR380 pKa = 11.84GMIGRR385 pKa = 11.84AFGGLGKK392 pKa = 9.76PFSLPEE398 pKa = 4.47AIKK401 pKa = 10.82

Molecular weight: 44.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1503

254

495

375.8

42.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.051 ± 0.617

2.129 ± 0.739

4.657 ± 0.482

5.522 ± 0.78

3.061 ± 0.428

6.587 ± 0.809

2.262 ± 0.785

4.125 ± 0.621

6.188 ± 0.855

7.585 ± 0.824

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.063 ± 0.351

3.992 ± 0.278

5.522 ± 0.762

3.859 ± 0.589

9.115 ± 0.699

7.984 ± 0.6

7.319 ± 1.332

5.921 ± 0.55

1.264 ± 0.197

2.794 ± 0.645
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski