Amino acid dipepetide frequency for Sphaerospermopsis kisseleviana NIES-73

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.693AlaAla: 6.693 ± 0.097	0.747AlaCys: 0.747 ± 0.024	3.883AlaAsp: 3.883 ± 0.057	5.097AlaGlu: 5.097 ± 0.067	2.695AlaPhe: 2.695 ± 0.041	4.934AlaGly: 4.934 ± 0.078	1.157AlaHis: 1.157 ± 0.03	6.308AlaIle: 6.308 ± 0.076	4.36AlaLys: 4.36 ± 0.061	7.418AlaLeu: 7.418 ± 0.089	1.516AlaMet: 1.516 ± 0.032	3.436AlaAsn: 3.436 ± 0.063	2.555AlaPro: 2.555 ± 0.052	3.528AlaGln: 3.528 ± 0.058	3.07AlaArg: 3.07 ± 0.051	3.984AlaSer: 3.984 ± 0.057	4.118AlaThr: 4.118 ± 0.06	5.136AlaVal: 5.136 ± 0.059	0.956AlaTrp: 0.956 ± 0.03	2.231AlaTyr: 2.231 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.618CysAla: 0.618 ± 0.023	0.162CysCys: 0.162 ± 0.011	0.549CysAsp: 0.549 ± 0.019	0.552CysGlu: 0.552 ± 0.02	0.429CysPhe: 0.429 ± 0.017	0.816CysGly: 0.816 ± 0.026	0.29CysHis: 0.29 ± 0.014	0.633CysIle: 0.633 ± 0.023	0.377CysLys: 0.377 ± 0.016	1.164CysLeu: 1.164 ± 0.034	0.182CysMet: 0.182 ± 0.012	0.393CysAsn: 0.393 ± 0.016	0.543CysPro: 0.543 ± 0.019	0.639CysGln: 0.639 ± 0.023	0.504CysArg: 0.504 ± 0.02	0.576CysSer: 0.576 ± 0.02	0.468CysThr: 0.468 ± 0.016	0.554CysVal: 0.554 ± 0.023	0.144CysTrp: 0.144 ± 0.011	0.379CysTyr: 0.379 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	3.456AspAla: 3.456 ± 0.054	0.494AspCys: 0.494 ± 0.02	2.358AspAsp: 2.358 ± 0.046	3.099AspGlu: 3.099 ± 0.043	2.486AspPhe: 2.486 ± 0.042	3.02AspGly: 3.02 ± 0.052	0.876AspHis: 0.876 ± 0.022	3.901AspIle: 3.901 ± 0.062	2.692AspLys: 2.692 ± 0.052	5.717AspLeu: 5.717 ± 0.063	0.814AspMet: 0.814 ± 0.022	2.208AspAsn: 2.208 ± 0.04	2.197AspPro: 2.197 ± 0.041	1.899AspGln: 1.899 ± 0.041	2.525AspArg: 2.525 ± 0.047	2.727AspSer: 2.727 ± 0.041	2.486AspThr: 2.486 ± 0.056	3.098AspVal: 3.098 ± 0.049	0.894AspTrp: 0.894 ± 0.024	1.898AspTyr: 1.898 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	4.783GluAla: 4.783 ± 0.073	0.52GluCys: 0.52 ± 0.021	2.941GluAsp: 2.941 ± 0.048	4.468GluGlu: 4.468 ± 0.068	2.669GluPhe: 2.669 ± 0.046	3.084GluGly: 3.084 ± 0.046	0.971GluHis: 0.971 ± 0.029	5.919GluIle: 5.919 ± 0.077	4.568GluLys: 4.568 ± 0.06	7.286GluLeu: 7.286 ± 0.087	1.48GluMet: 1.48 ± 0.034	3.872GluAsn: 3.872 ± 0.071	2.252GluPro: 2.252 ± 0.042	3.48GluGln: 3.48 ± 0.06	3.12GluArg: 3.12 ± 0.053	3.397GluSer: 3.397 ± 0.045	3.838GluThr: 3.838 ± 0.055	4.324GluVal: 4.324 ± 0.059	0.793GluTrp: 0.793 ± 0.026	2.227GluTyr: 2.227 ± 0.044	0.0GluXaa: 0.0 ± 0.0
Phe	2.933PheAla: 2.933 ± 0.051	0.562PheCys: 0.562 ± 0.019	2.252PheAsp: 2.252 ± 0.039	2.095PheGlu: 2.095 ± 0.042	1.699PhePhe: 1.699 ± 0.032	2.807PheGly: 2.807 ± 0.057	0.836PheHis: 0.836 ± 0.025	2.732PheIle: 2.732 ± 0.049	1.754PheLys: 1.754 ± 0.041	4.267PheLeu: 4.267 ± 0.068	0.745PheMet: 0.745 ± 0.021	1.956PheAsn: 1.956 ± 0.041	1.966PhePro: 1.966 ± 0.04	1.836PheGln: 1.836 ± 0.038	1.679PheArg: 1.679 ± 0.035	2.875PheSer: 2.875 ± 0.047	2.5PheThr: 2.5 ± 0.043	2.395PheVal: 2.395 ± 0.047	0.706PheTrp: 0.706 ± 0.026	1.461PheTyr: 1.461 ± 0.034	0.003PheXaa: 0.003 ± 0.003
Gly	4.198GlyAla: 4.198 ± 0.074	0.765GlyCys: 0.765 ± 0.029	3.149GlyAsp: 3.149 ± 0.053	4.211GlyGlu: 4.211 ± 0.055	2.878GlyPhe: 2.878 ± 0.054	4.422GlyGly: 4.422 ± 0.075	1.09GlyHis: 1.09 ± 0.03	5.126GlyIle: 5.126 ± 0.069	4.591GlyLys: 4.591 ± 0.057	6.596GlyLeu: 6.596 ± 0.078	1.5GlyMet: 1.5 ± 0.035	3.156GlyAsn: 3.156 ± 0.084	1.15GlyPro: 1.15 ± 0.036	2.49GlyGln: 2.49 ± 0.039	2.936GlyArg: 2.936 ± 0.055	3.76GlySer: 3.76 ± 0.06	3.523GlyThr: 3.523 ± 0.066	4.524GlyVal: 4.524 ± 0.064	1.054GlyTrp: 1.054 ± 0.031	2.279GlyTyr: 2.279 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	0.999HisAla: 0.999 ± 0.026	0.263HisCys: 0.263 ± 0.014	0.794HisAsp: 0.794 ± 0.025	0.972HisGlu: 0.972 ± 0.028	0.795HisPhe: 0.795 ± 0.024	1.106HisGly: 1.106 ± 0.032	0.635HisHis: 0.635 ± 0.027	1.284HisIle: 1.284 ± 0.03	0.919HisLys: 0.919 ± 0.033	2.24HisLeu: 2.24 ± 0.045	0.14HisMet: 0.14 ± 0.009	0.829HisAsn: 0.829 ± 0.024	1.351HisPro: 1.351 ± 0.036	1.235HisGln: 1.235 ± 0.03	1.012HisArg: 1.012 ± 0.024	1.158HisSer: 1.158 ± 0.033	0.932HisThr: 0.932 ± 0.026	0.769HisVal: 0.769 ± 0.026	0.341HisTrp: 0.341 ± 0.016	0.664HisTyr: 0.664 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	6.729IleAla: 6.729 ± 0.073	0.825IleCys: 0.825 ± 0.025	3.975IleAsp: 3.975 ± 0.053	4.723IleGlu: 4.723 ± 0.064	2.95IlePhe: 2.95 ± 0.048	4.499IleGly: 4.499 ± 0.068	1.353IleHis: 1.353 ± 0.031	5.018IleIle: 5.018 ± 0.064	4.123IleLys: 4.123 ± 0.061	7.48IleLeu: 7.48 ± 0.078	1.051IleMet: 1.051 ± 0.03	3.906IleAsn: 3.906 ± 0.065	3.902IlePro: 3.902 ± 0.049	3.277IleGln: 3.277 ± 0.051	3.082IleArg: 3.082 ± 0.045	5.049IleSer: 5.049 ± 0.064	4.423IleThr: 4.423 ± 0.072	4.284IleVal: 4.284 ± 0.057	0.974IleTrp: 0.974 ± 0.027	2.32IleTyr: 2.32 ± 0.044	0.0IleXaa: 0.0 ± 0.0
Lys	4.039LysAla: 4.039 ± 0.059	0.391LysCys: 0.391 ± 0.018	2.49LysAsp: 2.49 ± 0.048	3.43LysGlu: 3.43 ± 0.057	2.133LysPhe: 2.133 ± 0.036	2.793LysGly: 2.793 ± 0.049	0.931LysHis: 0.931 ± 0.026	4.706LysIle: 4.706 ± 0.066	3.064LysLys: 3.064 ± 0.06	6.312LysLeu: 6.312 ± 0.078	1.151LysMet: 1.151 ± 0.03	2.974LysAsn: 2.974 ± 0.055	2.745LysPro: 2.745 ± 0.056	3.09LysGln: 3.09 ± 0.05	2.507LysArg: 2.507 ± 0.046	3.602LysSer: 3.602 ± 0.058	3.459LysThr: 3.459 ± 0.052	3.391LysVal: 3.391 ± 0.048	0.668LysTrp: 0.668 ± 0.024	1.879LysTyr: 1.879 ± 0.035	0.0LysXaa: 0.0 ± 0.0
Leu	8.883LeuAla: 8.883 ± 0.095	0.983LeuCys: 0.983 ± 0.027	5.216LeuAsp: 5.216 ± 0.058	7.671LeuGlu: 7.671 ± 0.082	3.827LeuPhe: 3.827 ± 0.052	7.384LeuGly: 7.384 ± 0.084	1.836LeuHis: 1.836 ± 0.035	7.245LeuIle: 7.245 ± 0.071	6.011LeuLys: 6.011 ± 0.07	10.966LeuLeu: 10.966 ± 0.112	2.072LeuMet: 2.072 ± 0.041	5.012LeuAsn: 5.012 ± 0.072	5.638LeuPro: 5.638 ± 0.071	5.715LeuGln: 5.715 ± 0.072	5.119LeuArg: 5.119 ± 0.06	7.055LeuSer: 7.055 ± 0.084	6.478LeuThr: 6.478 ± 0.075	6.785LeuVal: 6.785 ± 0.078	1.362LeuTrp: 1.362 ± 0.034	2.747LeuTyr: 2.747 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	1.499MetAla: 1.499 ± 0.03	0.12MetCys: 0.12 ± 0.01	0.732MetAsp: 0.732 ± 0.022	1.051MetGlu: 1.051 ± 0.027	0.596MetPhe: 0.596 ± 0.021	1.353MetGly: 1.353 ± 0.032	0.261MetHis: 0.261 ± 0.014	1.329MetIle: 1.329 ± 0.031	1.049MetLys: 1.049 ± 0.029	1.974MetLeu: 1.974 ± 0.037	0.487MetMet: 0.487 ± 0.02	1.021MetAsn: 1.021 ± 0.03	0.815MetPro: 0.815 ± 0.025	0.96MetGln: 0.96 ± 0.025	0.919MetArg: 0.919 ± 0.029	1.377MetSer: 1.377 ± 0.03	1.397MetThr: 1.397 ± 0.033	1.269MetVal: 1.269 ± 0.03	0.168MetTrp: 0.168 ± 0.011	0.413MetTyr: 0.413 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.971AsnAla: 2.971 ± 0.05	0.527AsnCys: 0.527 ± 0.022	1.855AsnAsp: 1.855 ± 0.043	2.25AsnGlu: 2.25 ± 0.041	2.267AsnPhe: 2.267 ± 0.043	2.689AsnGly: 2.689 ± 0.054	1.075AsnHis: 1.075 ± 0.029	3.699AsnIle: 3.699 ± 0.059	2.458AsnLys: 2.458 ± 0.051	6.049AsnLeu: 6.049 ± 0.075	0.791AsnMet: 0.791 ± 0.02	2.829AsnAsn: 2.829 ± 0.064	2.849AsnPro: 2.849 ± 0.045	3.135AsnGln: 3.135 ± 0.059	2.447AsnArg: 2.447 ± 0.046	3.317AsnSer: 3.317 ± 0.059	2.677AsnThr: 2.677 ± 0.044	2.41AsnVal: 2.41 ± 0.045	0.871AsnTrp: 0.871 ± 0.025	1.867AsnTyr: 1.867 ± 0.04	0.0AsnXaa: 0.0 ± 0.0
Pro	3.022ProAla: 3.022 ± 0.053	0.367ProCys: 0.367 ± 0.015	2.919ProAsp: 2.919 ± 0.053	4.167ProGlu: 4.167 ± 0.055	1.649ProPhe: 1.649 ± 0.037	3.009ProGly: 3.009 ± 0.05	0.97ProHis: 0.97 ± 0.025	3.139ProIle: 3.139 ± 0.047	2.374ProLys: 2.374 ± 0.043	4.409ProLeu: 4.409 ± 0.062	0.665ProMet: 0.665 ± 0.022	2.234ProAsn: 2.234 ± 0.038	2.173ProPro: 2.173 ± 0.051	2.698ProGln: 2.698 ± 0.05	1.623ProArg: 1.623 ± 0.032	2.609ProSer: 2.609 ± 0.05	2.738ProThr: 2.738 ± 0.056	3.357ProVal: 3.357 ± 0.05	0.602ProTrp: 0.602 ± 0.021	1.307ProTyr: 1.307 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	4.11GlnAla: 4.11 ± 0.064	0.347GlnCys: 0.347 ± 0.017	2.205GlnAsp: 2.205 ± 0.04	4.139GlnGlu: 4.139 ± 0.064	1.728GlnPhe: 1.728 ± 0.034	3.108GlnGly: 3.108 ± 0.047	0.85GlnHis: 0.85 ± 0.022	3.947GlnIle: 3.947 ± 0.06	3.304GlnLys: 3.304 ± 0.053	5.605GlnLeu: 5.605 ± 0.079	1.05GlnMet: 1.05 ± 0.03	2.474GlnAsn: 2.474 ± 0.046	2.593GlnPro: 2.593 ± 0.048	3.591GlnGln: 3.591 ± 0.063	2.723GlnArg: 2.723 ± 0.047	2.719GlnSer: 2.719 ± 0.045	2.957GlnThr: 2.957 ± 0.048	3.66GlnVal: 3.66 ± 0.059	0.641GlnTrp: 0.641 ± 0.02	1.278GlnTyr: 1.278 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	2.661ArgAla: 2.661 ± 0.043	0.489ArgCys: 0.489 ± 0.019	2.285ArgAsp: 2.285 ± 0.041	3.273ArgGlu: 3.273 ± 0.056	2.021ArgPhe: 2.021 ± 0.04	2.718ArgGly: 2.718 ± 0.045	0.917ArgHis: 0.917 ± 0.028	3.269ArgIle: 3.269 ± 0.052	2.527ArgLys: 2.527 ± 0.05	5.418ArgLeu: 5.418 ± 0.066	0.97ArgMet: 0.97 ± 0.027	2.203ArgAsn: 2.203 ± 0.039	1.883ArgPro: 1.883 ± 0.037	2.731ArgGln: 2.731 ± 0.047	2.734ArgArg: 2.734 ± 0.045	2.831ArgSer: 2.831 ± 0.047	2.296ArgThr: 2.296 ± 0.047	3.022ArgVal: 3.022 ± 0.054	0.741ArgTrp: 0.741 ± 0.024	1.832ArgTyr: 1.832 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	3.923SerAla: 3.923 ± 0.059	0.656SerCys: 0.656 ± 0.018	2.946SerAsp: 2.946 ± 0.045	3.933SerGlu: 3.933 ± 0.056	2.375SerPhe: 2.375 ± 0.044	4.234SerGly: 4.234 ± 0.055	1.296SerHis: 1.296 ± 0.034	3.964SerIle: 3.964 ± 0.064	2.87SerLys: 2.87 ± 0.057	7.19SerLeu: 7.19 ± 0.078	1.124SerMet: 1.124 ± 0.03	2.767SerAsn: 2.767 ± 0.047	3.28SerPro: 3.28 ± 0.056	3.721SerGln: 3.721 ± 0.053	3.0SerArg: 3.0 ± 0.048	4.078SerSer: 4.078 ± 0.066	3.284SerThr: 3.284 ± 0.044	3.855SerVal: 3.855 ± 0.052	0.929SerTrp: 0.929 ± 0.03	1.873SerTyr: 1.873 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	4.614ThrAla: 4.614 ± 0.069	0.491ThrCys: 0.491 ± 0.02	2.757ThrAsp: 2.757 ± 0.049	3.59ThrGlu: 3.59 ± 0.06	2.133ThrPhe: 2.133 ± 0.05	4.261ThrGly: 4.261 ± 0.074	1.009ThrHis: 1.009 ± 0.029	4.022ThrIle: 4.022 ± 0.06	2.7ThrLys: 2.7 ± 0.051	6.048ThrLeu: 6.048 ± 0.086	0.832ThrMet: 0.832 ± 0.024	2.519ThrAsn: 2.519 ± 0.047	3.483ThrPro: 3.483 ± 0.057	2.881ThrGln: 2.881 ± 0.049	2.288ThrArg: 2.288 ± 0.045	3.494ThrSer: 3.494 ± 0.059	3.495ThrThr: 3.495 ± 0.071	3.883ThrVal: 3.883 ± 0.066	0.743ThrTrp: 0.743 ± 0.023	1.665ThrTyr: 1.665 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	4.989ValAla: 4.989 ± 0.066	0.669ValCys: 0.669 ± 0.023	3.342ValAsp: 3.342 ± 0.047	4.369ValGlu: 4.369 ± 0.062	2.633ValPhe: 2.633 ± 0.051	4.196ValGly: 4.196 ± 0.067	0.994ValHis: 0.994 ± 0.031	4.764ValIle: 4.764 ± 0.061	3.811ValLys: 3.811 ± 0.047	6.264ValLeu: 6.264 ± 0.071	1.415ValMet: 1.415 ± 0.031	3.212ValAsn: 3.212 ± 0.052	2.676ValPro: 2.676 ± 0.051	2.594ValGln: 2.594 ± 0.042	2.921ValArg: 2.921 ± 0.049	4.0ValSer: 4.0 ± 0.055	3.665ValThr: 3.665 ± 0.058	4.573ValVal: 4.573 ± 0.058	0.8ValTrp: 0.8 ± 0.023	1.956ValTyr: 1.956 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	0.727TrpAla: 0.727 ± 0.027	0.163TrpCys: 0.163 ± 0.009	0.674TrpAsp: 0.674 ± 0.024	1.053TrpGlu: 1.053 ± 0.028	0.613TrpPhe: 0.613 ± 0.025	0.958TrpGly: 0.958 ± 0.028	0.309TrpHis: 0.309 ± 0.015	0.864TrpIle: 0.864 ± 0.022	0.701TrpLys: 0.701 ± 0.022	1.86TrpLeu: 1.86 ± 0.043	0.351TrpMet: 0.351 ± 0.016	0.646TrpAsn: 0.646 ± 0.024	0.29TrpPro: 0.29 ± 0.015	1.239TrpGln: 1.239 ± 0.029	0.791TrpArg: 0.791 ± 0.022	0.734TrpSer: 0.734 ± 0.02	0.576TrpThr: 0.576 ± 0.021	0.928TrpVal: 0.928 ± 0.029	0.224TrpTrp: 0.224 ± 0.012	0.443TrpTyr: 0.443 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.974TyrAla: 1.974 ± 0.037	0.432TyrCys: 0.432 ± 0.021	1.522TyrAsp: 1.522 ± 0.036	1.851TyrGlu: 1.851 ± 0.043	1.483TyrPhe: 1.483 ± 0.036	2.007TyrGly: 2.007 ± 0.038	0.783TyrHis: 0.783 ± 0.022	2.074TyrIle: 2.074 ± 0.039	1.499TyrLys: 1.499 ± 0.035	3.742TyrLeu: 3.742 ± 0.048	0.452TyrMet: 0.452 ± 0.019	1.386TyrAsn: 1.386 ± 0.037	1.69TyrPro: 1.69 ± 0.035	2.293TyrGln: 2.293 ± 0.045	1.821TyrArg: 1.821 ± 0.037	1.957TyrSer: 1.957 ± 0.042	1.594TyrThr: 1.594 ± 0.038	1.626TyrVal: 1.626 ± 0.036	0.533TyrTrp: 0.533 ± 0.019	1.148TyrTyr: 1.148 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.003XaaThr: 0.003 ± 0.003	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5038 proteins (1454114 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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