Pomona bat hepatitis B virus
Average proteome isoelectric point is 8.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A7DQ32|A0A0A7DQ32_9HEPA DNA-directed DNA polymerase OS=Pomona bat hepatitis B virus OX=2049933 PE=3 SV=1
MM1 pKa = 7.76 AARR4 pKa = 11.84 LYY6 pKa = 11.12 CEE8 pKa = 5.34 LDD10 pKa = 3.32 PSRR13 pKa = 11.84 DD14 pKa = 3.65 VLLLRR19 pKa = 11.84 PFGAQPGGRR28 pKa = 11.84 PVSRR32 pKa = 11.84 FPGDD36 pKa = 3.76 SNGSTASTVPPVHH49 pKa = 6.39 RR50 pKa = 11.84 QDD52 pKa = 3.1 FALRR56 pKa = 11.84 RR57 pKa = 11.84 LPACAYY63 pKa = 10.34 SNAGPCVLRR72 pKa = 11.84 FTCAEE77 pKa = 3.74 AAVNMEE83 pKa = 3.9 TTMTNFVTWHH93 pKa = 5.34 TARR96 pKa = 11.84 QRR98 pKa = 11.84 GTVTQTLNSWQWYY111 pKa = 8.95 FGQLLMNQWEE121 pKa = 4.34 EE122 pKa = 4.25 TGWDD126 pKa = 3.64 DD127 pKa = 5.84 RR128 pKa = 11.84 IIAYY132 pKa = 10.07 VLGGCRR138 pKa = 11.84 HH139 pKa = 6.37 KK140 pKa = 11.13 LRR142 pKa = 5.47
Molecular weight: 16.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.192
IPC2_protein 8.024
IPC_protein 8.068
Toseland 7.527
ProMoST 8.712
Dawson 8.551
Bjellqvist 9.004
Wikipedia 8.478
Rodwell 8.536
Grimsley 7.424
Solomon 8.756
Lehninger 8.77
Nozaki 9.224
DTASelect 8.668
Thurlkill 8.697
EMBOSS 8.77
Sillero 9.063
Patrickios 4.342
IPC_peptide 8.741
IPC2_peptide 8.829
IPC2.peptide.svr19 8.977
Protein with the highest isoelectric point:
>tr|A0A0A7DQC0|A0A0A7DQC0_9HEPA External core antigen OS=Pomona bat hepatitis B virus OX=2049933 GN=C PE=3 SV=1
MM1 pKa = 7.55 HH2 pKa = 7.82 PFSQLFQSIQSLEE15 pKa = 4.11 EE16 pKa = 4.42 GEE18 pKa = 4.59 VEE20 pKa = 4.27 QLLGPPEE27 pKa = 4.38 DD28 pKa = 4.57 ALPLLADD35 pKa = 3.84 ANLNHH40 pKa = 6.66 RR41 pKa = 11.84 VAEE44 pKa = 4.07 EE45 pKa = 4.32 LLLQLPLHH53 pKa = 6.24 AEE55 pKa = 3.76 IWVHH59 pKa = 6.08 KK60 pKa = 10.62 AGGLTGLYY68 pKa = 9.74 SEE70 pKa = 5.12 KK71 pKa = 10.54 PKK73 pKa = 10.56 IFNPEE78 pKa = 3.15 WKK80 pKa = 8.77 TPSFPNIHH88 pKa = 6.47 LRR90 pKa = 11.84 HH91 pKa = 6.63 DD92 pKa = 3.78 LCEE95 pKa = 5.37 HH96 pKa = 5.87 IDD98 pKa = 3.61 SRR100 pKa = 11.84 IGLTKK105 pKa = 10.22 GEE107 pKa = 3.86 KK108 pKa = 9.78 RR109 pKa = 11.84 RR110 pKa = 11.84 FKK112 pKa = 10.82 LCLPARR118 pKa = 11.84 FFPNKK123 pKa = 8.53 TKK125 pKa = 10.77 YY126 pKa = 10.22 FPLHH130 pKa = 5.72 NAIKK134 pKa = 10.3 NYY136 pKa = 10.35 YY137 pKa = 8.12 PDD139 pKa = 3.18 HH140 pKa = 6.37 TLTHH144 pKa = 6.93 FFHH147 pKa = 6.52 TAHH150 pKa = 6.33 YY151 pKa = 10.36 LWTLWEE157 pKa = 4.25 SGVTYY162 pKa = 10.69 LRR164 pKa = 11.84 TSQSSASFNGQSYY177 pKa = 8.76 PWEE180 pKa = 4.46 QKK182 pKa = 9.78 HH183 pKa = 5.4 HH184 pKa = 4.65 THH186 pKa = 6.75 GKK188 pKa = 7.25 QQISSQSLRR197 pKa = 11.84 LPPRR201 pKa = 11.84 SPATNSPKK209 pKa = 9.96 FRR211 pKa = 11.84 GLGKK215 pKa = 10.18 ILGPKK220 pKa = 8.23 SAKK223 pKa = 10.15 GSLAPSIGTLSGSLGTKK240 pKa = 10.07 SSPSSWGSTWNRR252 pKa = 11.84 RR253 pKa = 11.84 EE254 pKa = 4.09 FSGSPYY260 pKa = 10.11 QYY262 pKa = 10.6 QKK264 pKa = 11.06 YY265 pKa = 9.48 RR266 pKa = 11.84 RR267 pKa = 11.84 NNQNYY272 pKa = 8.58 YY273 pKa = 9.26 RR274 pKa = 11.84 QKK276 pKa = 7.76 TTNNYY281 pKa = 9.36 YY282 pKa = 10.58 KK283 pKa = 10.61 NKK285 pKa = 9.23 QDD287 pKa = 4.88 QSIYY291 pKa = 9.64 WNNANKK297 pKa = 9.9 TNITSHH303 pKa = 6.27 TSIQSPKK310 pKa = 10.06 GASTNAPDD318 pKa = 3.62 ITSKK322 pKa = 9.96 TDD324 pKa = 2.9 SSTSQYY330 pKa = 10.62 EE331 pKa = 3.76 QDD333 pKa = 3.91 LEE335 pKa = 4.63 TEE337 pKa = 4.3 VRR339 pKa = 11.84 QPDD342 pKa = 3.96 NSCPWLFFNHH352 pKa = 5.97 SPACGRR358 pKa = 11.84 YY359 pKa = 9.31 CLRR362 pKa = 11.84 QCLLLLSDD370 pKa = 4.24 WGPCHH375 pKa = 6.75 RR376 pKa = 11.84 HH377 pKa = 5.42 GDD379 pKa = 3.72 HH380 pKa = 8.01 LIRR383 pKa = 11.84 TPRR386 pKa = 11.84 TPRR389 pKa = 11.84 RR390 pKa = 11.84 ISGGVFLVDD399 pKa = 3.54 KK400 pKa = 10.57 NPNNRR405 pKa = 11.84 TEE407 pKa = 3.84 SRR409 pKa = 11.84 LVVDD413 pKa = 5.53 FSQFSRR419 pKa = 11.84 GNTRR423 pKa = 11.84 VQWPKK428 pKa = 10.19 FAVPNLQALTNLLSSNLSWLSLDD451 pKa = 3.44 VSAAFYY457 pKa = 9.93 HH458 pKa = 6.73 IPLSPAAMPHH468 pKa = 6.39 LLVGSPGLEE477 pKa = 3.7 GLASCMSPKK486 pKa = 9.38 STNNNHH492 pKa = 6.07 QEE494 pKa = 4.08 MQHH497 pKa = 5.46 LHH499 pKa = 5.34 SLCGRR504 pKa = 11.84 RR505 pKa = 11.84 NLLGLLLLFKK515 pKa = 9.84 TCRR518 pKa = 11.84 RR519 pKa = 11.84 KK520 pKa = 8.58 QTLLGHH526 pKa = 5.9 SFFKK530 pKa = 10.76 GFRR533 pKa = 11.84 KK534 pKa = 9.91 IPTGVGLSPILLAPFPSALASVVRR558 pKa = 11.84 RR559 pKa = 11.84 TFPHH563 pKa = 6.58 CLAFTYY569 pKa = 10.09 MDD571 pKa = 4.38 DD572 pKa = 3.8 VVLGATTPPHH582 pKa = 6.63 LEE584 pKa = 3.64 SVYY587 pKa = 11.26 ASVCSLFSDD596 pKa = 4.42 LGIVLNPDD604 pKa = 2.8 KK605 pKa = 10.83 TKK607 pKa = 9.36 WWGKK611 pKa = 8.82 HH612 pKa = 4.06 LQFMGLLITPSGTLPQSKK630 pKa = 7.16 QQKK633 pKa = 8.22 WAIQLLKK640 pKa = 10.68 ALPCYY645 pKa = 10.25 TIFDD649 pKa = 3.79 WKK651 pKa = 10.31 ILQRR655 pKa = 11.84 LTGLLGFLAPFTACGYY671 pKa = 7.21 PALMPLYY678 pKa = 10.4 HH679 pKa = 7.76 AITQKK684 pKa = 10.97 NGFEE688 pKa = 4.06 FSPAYY693 pKa = 9.78 KK694 pKa = 10.59 GFLLRR699 pKa = 11.84 LYY701 pKa = 10.38 SRR703 pKa = 11.84 PLHH706 pKa = 5.4 VVRR709 pKa = 11.84 QRR711 pKa = 11.84 SSTCQVFADD720 pKa = 4.26 ATPTGWGLVNHH731 pKa = 5.94 QLALYY736 pKa = 10.16 KK737 pKa = 10.21 SGRR740 pKa = 11.84 FPRR743 pKa = 11.84 PLPIHH748 pKa = 5.89 HH749 pKa = 7.41 AEE751 pKa = 4.71 LIAAILARR759 pKa = 11.84 CWSGAKK765 pKa = 10.49 LLGTDD770 pKa = 3.34 NTIVCSHH777 pKa = 6.99 KK778 pKa = 9.65 FTHH781 pKa = 6.59 FPWLLGCTANWILRR795 pKa = 11.84 EE796 pKa = 3.91 TSFCYY801 pKa = 10.56 VPSEE805 pKa = 4.58 LNPADD810 pKa = 3.74 APSRR814 pKa = 11.84 GFLGIRR820 pKa = 11.84 TAPPPLRR827 pKa = 11.84 FRR829 pKa = 11.84 PSTGRR834 pKa = 11.84 TSLFVVSPPAPTRR847 pKa = 11.84 MPDD850 pKa = 2.88 RR851 pKa = 11.84 VSFVSPVQKK860 pKa = 10.5 RR861 pKa = 11.84 LSTWRR866 pKa = 11.84 PPP868 pKa = 3.22
Molecular weight: 97.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.207
IPC2_protein 9.238
IPC_protein 9.209
Toseland 9.838
ProMoST 9.604
Dawson 10.087
Bjellqvist 9.823
Wikipedia 10.262
Rodwell 10.423
Grimsley 10.16
Solomon 10.101
Lehninger 10.072
Nozaki 9.955
DTASelect 9.78
Thurlkill 9.94
EMBOSS 10.262
Sillero 10.028
Patrickios 7.556
IPC_peptide 10.101
IPC2_peptide 8.741
IPC2.peptide.svr19 8.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1642
142
868
410.5
46.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.725 ± 0.382
2.741 ± 0.338
2.862 ± 0.147
2.984 ± 0.392
4.75 ± 0.279
6.395 ± 0.344
3.228 ± 0.58
3.837 ± 0.39
4.019 ± 0.748
12.119 ± 0.488
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.34 ± 0.264
4.141 ± 0.402
8.709 ± 0.431
4.202 ± 0.233
6.456 ± 0.917
8.952 ± 0.493
7.734 ± 0.655
4.141 ± 0.47
3.167 ± 0.526
2.497 ± 0.426
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here