Pantoea phage vB_PagS_MED16

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S9U7I2|A0A3S9U7I2_9CAUD Uncharacterized protein OS=Pantoea phage vB_PagS_MED16 OX=2499074 GN=MED16_gp35 PE=4 SV=1
MM1 pKa = 7.5SNVLIQQALEE11 pKa = 3.75AALNDD16 pKa = 4.05FAASGGYY23 pKa = 8.23PVAWEE28 pKa = 4.03NTTFQPDD35 pKa = 3.4PDD37 pKa = 3.79AVYY40 pKa = 10.22LVPYY44 pKa = 8.78FMPNVTDD51 pKa = 6.06SIDD54 pKa = 3.59LQQKK58 pKa = 6.38TRR60 pKa = 11.84IYY62 pKa = 10.33QGIFQVMVVWPAGMGTIGAKK82 pKa = 9.98AVAEE86 pKa = 4.58SIAEE90 pKa = 3.95AFPNGWEE97 pKa = 4.06TLAGSVRR104 pKa = 11.84VYY106 pKa = 10.18TDD108 pKa = 3.77RR109 pKa = 11.84PAQVSAGLSDD119 pKa = 3.57DD120 pKa = 3.17VSYY123 pKa = 11.2NVPVSIYY130 pKa = 10.16YY131 pKa = 8.68RR132 pKa = 11.84TDD134 pKa = 2.47II135 pKa = 4.85

Molecular weight: 14.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S9U7N2|A0A3S9U7N2_9CAUD Uncharacterized protein OS=Pantoea phage vB_PagS_MED16 OX=2499074 GN=MED16_gp72 PE=4 SV=1
MM1 pKa = 7.55TKK3 pKa = 10.07PGLISGMIGITQTMRR18 pKa = 11.84QRR20 pKa = 11.84KK21 pKa = 9.46SMTQSRR27 pKa = 11.84EE28 pKa = 3.95QKK30 pKa = 9.49SGTRR34 pKa = 11.84RR35 pKa = 11.84PHH37 pKa = 4.73LTGYY41 pKa = 8.63SLNMIQRR48 pKa = 11.84LRR50 pKa = 11.84WLKK53 pKa = 10.88VGLLLLVVSLLGCAKK68 pKa = 10.36EE69 pKa = 4.39SVTPSPSYY77 pKa = 11.14SNNAAKK83 pKa = 10.51VPALNPSSLQGPAPEE98 pKa = 4.52FCSPSCLEE106 pKa = 4.12SLTKK110 pKa = 10.14QRR112 pKa = 11.84QTMQQRR118 pKa = 11.84LTAQGGQGG126 pKa = 3.15

Molecular weight: 13.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

14960

28

1091

204.9

22.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.0 ± 0.368

1.217 ± 0.134

5.989 ± 0.205

5.682 ± 0.348

3.262 ± 0.155

7.941 ± 0.324

1.858 ± 0.157

5.541 ± 0.211

5.354 ± 0.333

7.567 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.088 ± 0.153

4.472 ± 0.18

4.191 ± 0.277

4.532 ± 0.411

5.555 ± 0.274

6.11 ± 0.235

6.07 ± 0.284

6.845 ± 0.202

1.765 ± 0.152

2.961 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski