Schistosoma japonicum (Blood fluke)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Spiralia; Lophotrochozoa; Platyhelminthes; Trematoda; Digenea; Strigeidida; Schistosomatoidea; Schistosomatidae; Schistosoma

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14733 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Z2DK85|A0A4Z2DK85_SCHJA Uncharacterized protein OS=Schistosoma japonicum OX=6182 GN=EWB00_000056 PE=4 SV=1
MM1 pKa = 7.23MSIILCLIILSYY13 pKa = 10.49IISFALMEE21 pKa = 5.52LIVEE25 pKa = 4.3PLVVWKK31 pKa = 10.62YY32 pKa = 10.95GKK34 pKa = 9.53NRR36 pKa = 11.84NDD38 pKa = 3.67GYY40 pKa = 11.56VVVTDD45 pKa = 4.1VNNHH49 pKa = 4.77NHH51 pKa = 6.33NDD53 pKa = 3.71NDD55 pKa = 5.31DD56 pKa = 4.25DD57 pKa = 5.49DD58 pKa = 7.16ADD60 pKa = 4.7ADD62 pKa = 4.02VTVMNKK68 pKa = 10.62DD69 pKa = 3.29NFIGNDD75 pKa = 3.04VCVNKK80 pKa = 10.16FDD82 pKa = 6.75DD83 pKa = 4.81IRR85 pKa = 11.84DD86 pKa = 3.67DD87 pKa = 4.43KK88 pKa = 11.7INVDD92 pKa = 3.52EE93 pKa = 5.05AGCGEE98 pKa = 4.11NEE100 pKa = 3.92EE101 pKa = 5.15NIDD104 pKa = 3.96NEE106 pKa = 4.46GEE108 pKa = 3.96NDD110 pKa = 3.47EE111 pKa = 5.34VRR113 pKa = 11.84DD114 pKa = 4.08NDD116 pKa = 3.71GDD118 pKa = 3.98DD119 pKa = 3.54GNEE122 pKa = 4.08YY123 pKa = 10.95GDD125 pKa = 3.57VAIDD129 pKa = 3.29IDD131 pKa = 4.27EE132 pKa = 4.57DD133 pKa = 4.08VAINTYY139 pKa = 11.06SDD141 pKa = 5.11FIDD144 pKa = 3.83NNADD148 pKa = 3.19AGTVVVDD155 pKa = 4.83TVAVAAGDD163 pKa = 4.08GFDD166 pKa = 3.46SHH168 pKa = 8.46VDD170 pKa = 3.19IVSSNGDD177 pKa = 3.38VVIDD181 pKa = 3.43IDD183 pKa = 4.67KK184 pKa = 11.37SVDD187 pKa = 2.93ACSDD191 pKa = 3.35YY192 pKa = 11.54DD193 pKa = 3.55ATAAASAVVASAAVAAHH210 pKa = 6.55YY211 pKa = 11.03DD212 pKa = 4.07DD213 pKa = 6.64DD214 pKa = 5.82DD215 pKa = 4.11VDD217 pKa = 4.05YY218 pKa = 11.44DD219 pKa = 4.27GNVSKK224 pKa = 11.02KK225 pKa = 10.23FRR227 pKa = 11.84WKK229 pKa = 9.36VFRR232 pKa = 11.84YY233 pKa = 8.12FCCCC237 pKa = 3.7

Molecular weight: 25.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C1L4U3|C1L4U3_SCHJA Glutaminyl-tRNA synthetase OS=Schistosoma japonicum OX=6182 GN=EWB00_006154 PE=2 SV=1
MM1 pKa = 7.39FGMRR5 pKa = 11.84FSPSKK10 pKa = 10.84CKK12 pKa = 9.85MLLQDD17 pKa = 4.61WLNSAPKK24 pKa = 10.19LVIGSEE30 pKa = 4.27TIEE33 pKa = 4.2CVNRR37 pKa = 11.84FTYY40 pKa = 10.29LGSLISPNGLVSDD53 pKa = 5.01EE54 pKa = 3.81ISARR58 pKa = 11.84IHH60 pKa = 6.08KK61 pKa = 10.2ARR63 pKa = 11.84SAFANLRR70 pKa = 11.84HH71 pKa = 5.92LWRR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84DD77 pKa = 3.14IRR79 pKa = 11.84LMTKK83 pKa = 10.17GRR85 pKa = 11.84VYY87 pKa = 10.32CAAVRR92 pKa = 11.84SVLLYY97 pKa = 10.92GCEE100 pKa = 3.78TWPLRR105 pKa = 11.84IEE107 pKa = 4.79DD108 pKa = 3.58IRR110 pKa = 11.84RR111 pKa = 11.84LLVFDD116 pKa = 4.77LRR118 pKa = 11.84CLRR121 pKa = 11.84NIARR125 pKa = 4.26

Molecular weight: 14.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10042

4691

14733

7781729

41

6097

528.2

59.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.809 ± 0.018

2.218 ± 0.012

5.311 ± 0.014

5.594 ± 0.023

3.904 ± 0.014

4.481 ± 0.028

2.906 ± 0.012

6.431 ± 0.018

5.594 ± 0.02

9.858 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.025 ± 0.008

6.668 ± 0.026

4.615 ± 0.019

4.276 ± 0.015

4.99 ± 0.016

10.175 ± 0.037

6.258 ± 0.02

5.582 ± 0.015

1.005 ± 0.006

3.298 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski