Tortoise microvirus 74
Average proteome isoelectric point is 7.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W6I5|A0A4P8W6I5_9VIRU Uncharacterized protein OS=Tortoise microvirus 74 OX=2583181 PE=4 SV=1
MM1 pKa = 7.21 SVKK4 pKa = 10.04 LRR6 pKa = 11.84 HH7 pKa = 5.07 LAKK10 pKa = 9.6 WAHH13 pKa = 5.89 LPAGEE18 pKa = 4.42 VIPLAGKK25 pKa = 9.96 KK26 pKa = 10.21 RR27 pKa = 11.84 IVTLEE32 pKa = 3.93 FNVDD36 pKa = 3.76 FPTRR40 pKa = 11.84 FDD42 pKa = 3.32 VLEE45 pKa = 4.38 NGKK48 pKa = 8.48 STLLRR53 pKa = 11.84 AVEE56 pKa = 3.94 PRR58 pKa = 11.84 EE59 pKa = 4.24 CPLKK63 pKa = 11.0 VSFAVEE69 pKa = 4.16 GDD71 pKa = 3.79 CAIVADD77 pKa = 4.36 TEE79 pKa = 4.58 GDD81 pKa = 3.84 VWWATDD87 pKa = 3.53 DD88 pKa = 4.71 GEE90 pKa = 4.44 VLSYY94 pKa = 10.6 EE95 pKa = 4.28 VEE97 pKa = 4.29 TEE99 pKa = 4.23 SFTKK103 pKa = 10.3 LQQRR107 pKa = 11.84 MEE109 pKa = 4.22 MTPEE113 pKa = 3.82 MEE115 pKa = 3.83 LTIYY119 pKa = 10.38 KK120 pKa = 10.02 AQLRR124 pKa = 11.84 AEE126 pKa = 3.91 QRR128 pKa = 11.84 ARR130 pKa = 11.84 EE131 pKa = 4.06 VAEE134 pKa = 4.03 LLLAKK139 pKa = 9.96 KK140 pKa = 9.87 QRR142 pKa = 11.84 EE143 pKa = 4.01 EE144 pKa = 4.27 AEE146 pKa = 4.08 AANADD151 pKa = 4.06 PEE153 pKa = 4.45 TGEE156 pKa = 4.64 VNDD159 pKa = 4.47 KK160 pKa = 11.12 NGAGLGQAAADD171 pKa = 4.04 GTSEE175 pKa = 4.5 AGSSAAPKK183 pKa = 9.76 PP184 pKa = 3.76
Molecular weight: 20.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.871
IPC2_protein 4.8
IPC_protein 4.698
Toseland 4.558
ProMoST 4.774
Dawson 4.622
Bjellqvist 4.774
Wikipedia 4.457
Rodwell 4.533
Grimsley 4.469
Solomon 4.622
Lehninger 4.571
Nozaki 4.724
DTASelect 4.825
Thurlkill 4.546
EMBOSS 4.482
Sillero 4.8
Patrickios 4.304
IPC_peptide 4.635
IPC2_peptide 4.8
IPC2.peptide.svr19 4.748
Protein with the highest isoelectric point:
>tr|A0A4P8W6U3|A0A4P8W6U3_9VIRU Uncharacterized protein OS=Tortoise microvirus 74 OX=2583181 PE=4 SV=1
MM1 pKa = 7.38 VNGKK5 pKa = 8.75 GAWKK9 pKa = 9.16 MFKK12 pKa = 10.34 QINDD16 pKa = 5.06 LILKK20 pKa = 8.83 PLSTRR25 pKa = 11.84 LGTMVTGGLIGLGANAQHH43 pKa = 6.91 ADD45 pKa = 3.14 WVGTGIAGGLLIGADD60 pKa = 4.87 LLAAWLRR67 pKa = 11.84 KK68 pKa = 9.57 RR69 pKa = 11.84 SIEE72 pKa = 3.97 RR73 pKa = 11.84 KK74 pKa = 9.21 AVAKK78 pKa = 10.46 AVASS82 pKa = 3.88
Molecular weight: 8.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.173
IPC2_protein 9.677
IPC_protein 9.882
Toseland 11.052
ProMoST 10.555
Dawson 11.082
Bjellqvist 10.687
Wikipedia 11.228
Rodwell 11.579
Grimsley 11.096
Solomon 11.199
Lehninger 11.184
Nozaki 11.008
DTASelect 10.687
Thurlkill 11.008
EMBOSS 11.433
Sillero 11.008
Patrickios 11.345
IPC_peptide 11.213
IPC2_peptide 8.99
IPC2.peptide.svr19 8.803
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1868
71
612
266.9
29.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.405 ± 0.95
1.071 ± 0.364
6.263 ± 0.543
6.585 ± 0.854
3.266 ± 0.461
10.011 ± 1.505
2.944 ± 0.25
2.998 ± 0.508
5.567 ± 0.594
7.013 ± 1.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.248 ± 0.609
3.64 ± 0.531
6.424 ± 0.403
4.497 ± 0.753
6.852 ± 0.952
4.925 ± 0.877
4.764 ± 0.754
7.334 ± 0.412
2.623 ± 0.632
2.57 ± 0.573
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here