Tortoise microvirus 74

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4P8W6I5|A0A4P8W6I5_9VIRU Uncharacterized protein OS=Tortoise microvirus 74 OX=2583181 PE=4 SV=1
MM1 pKa = 7.21SVKK4 pKa = 10.04LRR6 pKa = 11.84HH7 pKa = 5.07LAKK10 pKa = 9.6WAHH13 pKa = 5.89LPAGEE18 pKa = 4.42VIPLAGKK25 pKa = 9.96KK26 pKa = 10.21RR27 pKa = 11.84IVTLEE32 pKa = 3.93FNVDD36 pKa = 3.76FPTRR40 pKa = 11.84FDD42 pKa = 3.32VLEE45 pKa = 4.38NGKK48 pKa = 8.48STLLRR53 pKa = 11.84AVEE56 pKa = 3.94PRR58 pKa = 11.84EE59 pKa = 4.24CPLKK63 pKa = 11.0VSFAVEE69 pKa = 4.16GDD71 pKa = 3.79CAIVADD77 pKa = 4.36TEE79 pKa = 4.58GDD81 pKa = 3.84VWWATDD87 pKa = 3.53DD88 pKa = 4.71GEE90 pKa = 4.44VLSYY94 pKa = 10.6EE95 pKa = 4.28VEE97 pKa = 4.29TEE99 pKa = 4.23SFTKK103 pKa = 10.3LQQRR107 pKa = 11.84MEE109 pKa = 4.22MTPEE113 pKa = 3.82MEE115 pKa = 3.83LTIYY119 pKa = 10.38KK120 pKa = 10.02AQLRR124 pKa = 11.84AEE126 pKa = 3.91QRR128 pKa = 11.84ARR130 pKa = 11.84EE131 pKa = 4.06VAEE134 pKa = 4.03LLLAKK139 pKa = 9.96KK140 pKa = 9.87QRR142 pKa = 11.84EE143 pKa = 4.01EE144 pKa = 4.27AEE146 pKa = 4.08AANADD151 pKa = 4.06PEE153 pKa = 4.45TGEE156 pKa = 4.64VNDD159 pKa = 4.47KK160 pKa = 11.12NGAGLGQAAADD171 pKa = 4.04GTSEE175 pKa = 4.5AGSSAAPKK183 pKa = 9.76PP184 pKa = 3.76

Molecular weight:
20.11 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4P8W6U3|A0A4P8W6U3_9VIRU Uncharacterized protein OS=Tortoise microvirus 74 OX=2583181 PE=4 SV=1
MM1 pKa = 7.38VNGKK5 pKa = 8.75GAWKK9 pKa = 9.16MFKK12 pKa = 10.34QINDD16 pKa = 5.06LILKK20 pKa = 8.83PLSTRR25 pKa = 11.84LGTMVTGGLIGLGANAQHH43 pKa = 6.91ADD45 pKa = 3.14WVGTGIAGGLLIGADD60 pKa = 4.87LLAAWLRR67 pKa = 11.84KK68 pKa = 9.57RR69 pKa = 11.84SIEE72 pKa = 3.97RR73 pKa = 11.84KK74 pKa = 9.21AVAKK78 pKa = 10.46AVASS82 pKa = 3.88

Molecular weight:
8.57 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1868

71

612

266.9

29.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.405 ± 0.95

1.071 ± 0.364

6.263 ± 0.543

6.585 ± 0.854

3.266 ± 0.461

10.011 ± 1.505

2.944 ± 0.25

2.998 ± 0.508

5.567 ± 0.594

7.013 ± 1.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.609

3.64 ± 0.531

6.424 ± 0.403

4.497 ± 0.753

6.852 ± 0.952

4.925 ± 0.877

4.764 ± 0.754

7.334 ± 0.412

2.623 ± 0.632

2.57 ± 0.573

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski