Staphylococcus virus 42e
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4ZCV9|Q4ZCV9_9CAUD ORF064 OS=Staphylococcus virus 42e OX=320837 PE=4 SV=1
MM1 pKa = 7.17 MNNRR5 pKa = 11.84 EE6 pKa = 4.11 QIEE9 pKa = 3.98 QSVISASAYY18 pKa = 10.04 NGNDD22 pKa = 3.29 TEE24 pKa = 4.52 GLLKK28 pKa = 10.49 EE29 pKa = 4.44 IEE31 pKa = 4.24 DD32 pKa = 4.28 VYY34 pKa = 11.57 KK35 pKa = 10.64 KK36 pKa = 10.72 AQAFDD41 pKa = 4.66 EE42 pKa = 4.34 ILEE45 pKa = 4.2 GLPNAMQDD53 pKa = 3.61 ALKK56 pKa = 10.63 EE57 pKa = 4.3 DD58 pKa = 3.43 IGLDD62 pKa = 3.48 EE63 pKa = 5.32 AVGIMTGQVVYY74 pKa = 10.25 KK75 pKa = 10.48 YY76 pKa = 10.54 EE77 pKa = 4.02 EE78 pKa = 4.2 EE79 pKa = 4.27 QEE81 pKa = 4.06 SDD83 pKa = 3.01
Molecular weight: 9.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.211
IPC2_protein 3.935
IPC_protein 3.834
Toseland 3.668
ProMoST 3.897
Dawson 3.795
Bjellqvist 4.012
Wikipedia 3.694
Rodwell 3.681
Grimsley 3.579
Solomon 3.783
Lehninger 3.732
Nozaki 3.923
DTASelect 4.037
Thurlkill 3.706
EMBOSS 3.706
Sillero 3.948
Patrickios 3.427
IPC_peptide 3.783
IPC2_peptide 3.935
IPC2.peptide.svr19 3.893
Protein with the highest isoelectric point:
>tr|Q4ZCX5|Q4ZCX5_9CAUD ORF098 OS=Staphylococcus virus 42e OX=320837 PE=4 SV=1
MM1 pKa = 7.2 NVAILANPAIDD12 pKa = 3.52 HH13 pKa = 6.54 AVVKK17 pKa = 9.77 YY18 pKa = 10.59 RR19 pKa = 11.84 NGISTIAKK27 pKa = 6.56 TVKK30 pKa = 10.25 HH31 pKa = 5.3 STATRR36 pKa = 3.39
Molecular weight: 3.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.252
IPC2_protein 9.838
IPC_protein 10.248
Toseland 10.73
ProMoST 10.95
Dawson 10.818
Bjellqvist 10.452
Wikipedia 10.965
Rodwell 11.345
Grimsley 10.862
Solomon 10.891
Lehninger 10.877
Nozaki 10.687
DTASelect 10.452
Thurlkill 10.716
EMBOSS 11.111
Sillero 10.73
Patrickios 11.199
IPC_peptide 10.906
IPC2_peptide 9.033
IPC2.peptide.svr19 8.661
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
79
0
79
14688
33
2066
185.9
21.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.76 ± 0.524
0.517 ± 0.094
6.291 ± 0.281
7.523 ± 0.326
3.894 ± 0.232
5.964 ± 0.521
1.845 ± 0.148
7.19 ± 0.321
9.838 ± 0.407
8.068 ± 0.275
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.757 ± 0.152
6.59 ± 0.249
2.614 ± 0.209
3.969 ± 0.157
4.01 ± 0.167
6.182 ± 0.365
5.903 ± 0.239
5.678 ± 0.231
1.178 ± 0.133
4.228 ± 0.311
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here