Amino acid dipepetide frequency for Stx2 converting phage I

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.118AlaAla: 8.118 ± 0.705
1.755AlaCys: 1.755 ± 0.343
4.096AlaAsp: 4.096 ± 0.544
6.29AlaGlu: 6.29 ± 0.671
3.84AlaPhe: 3.84 ± 0.568
7.204AlaGly: 7.204 ± 0.802
1.609AlaHis: 1.609 ± 0.254
4.096AlaIle: 4.096 ± 0.377
4.059AlaLys: 4.059 ± 0.546
7.387AlaLeu: 7.387 ± 0.605
2.743AlaMet: 2.743 ± 0.376
2.779AlaAsn: 2.779 ± 0.31
3.657AlaPro: 3.657 ± 0.427
4.279AlaGln: 4.279 ± 0.573
5.193AlaArg: 5.193 ± 0.482
6.18AlaSer: 6.18 ± 0.657
4.864AlaThr: 4.864 ± 0.601
5.851AlaVal: 5.851 ± 0.52
1.572AlaTrp: 1.572 ± 0.235
1.902AlaTyr: 1.902 ± 0.279
0.0AlaXaa: 0.0 ± 0.0
Cys
1.755CysAla: 1.755 ± 0.277
0.951CysCys: 0.951 ± 0.222
0.622CysAsp: 0.622 ± 0.192
0.914CysGlu: 0.914 ± 0.214
0.951CysPhe: 0.951 ± 0.226
1.39CysGly: 1.39 ± 0.302
1.097CysHis: 1.097 ± 0.264
0.914CysIle: 0.914 ± 0.238
0.585CysLys: 0.585 ± 0.17
1.572CysLeu: 1.572 ± 0.353
0.475CysMet: 0.475 ± 0.132
0.585CysAsn: 0.585 ± 0.144
1.097CysPro: 1.097 ± 0.231
0.622CysGln: 0.622 ± 0.161
2.048CysArg: 2.048 ± 0.425
2.048CysSer: 2.048 ± 0.378
0.914CysThr: 0.914 ± 0.205
1.426CysVal: 1.426 ± 0.277
0.329CysTrp: 0.329 ± 0.1
0.366CysTyr: 0.366 ± 0.119
0.0CysXaa: 0.0 ± 0.0
Asp
4.644AspAla: 4.644 ± 0.547
0.805AspCys: 0.805 ± 0.179
3.072AspAsp: 3.072 ± 0.294
3.291AspGlu: 3.291 ± 0.343
1.499AspPhe: 1.499 ± 0.22
3.547AspGly: 3.547 ± 0.387
0.695AspHis: 0.695 ± 0.192
3.072AspIle: 3.072 ± 0.309
3.145AspLys: 3.145 ± 0.323
3.401AspLeu: 3.401 ± 0.474
1.499AspMet: 1.499 ± 0.241
2.158AspAsn: 2.158 ± 0.37
1.792AspPro: 1.792 ± 0.299
1.426AspGln: 1.426 ± 0.263
2.743AspArg: 2.743 ± 0.336
2.925AspSer: 2.925 ± 0.32
2.048AspThr: 2.048 ± 0.329
3.218AspVal: 3.218 ± 0.336
0.768AspTrp: 0.768 ± 0.191
1.097AspTyr: 1.097 ± 0.213
0.0AspXaa: 0.0 ± 0.0
Glu
5.083GluAla: 5.083 ± 0.488
1.17GluCys: 1.17 ± 0.249
1.682GluAsp: 1.682 ± 0.284
2.999GluGlu: 2.999 ± 0.336
2.158GluPhe: 2.158 ± 0.331
2.962GluGly: 2.962 ± 0.479
1.17GluHis: 1.17 ± 0.218
3.072GluIle: 3.072 ± 0.33
3.328GluLys: 3.328 ± 0.38
4.425GluLeu: 4.425 ± 0.476
1.609GluMet: 1.609 ± 0.237
2.304GluAsn: 2.304 ± 0.364
1.755GluPro: 1.755 ± 0.301
3.255GluGln: 3.255 ± 0.448
4.132GluArg: 4.132 ± 0.536
3.291GluSer: 3.291 ± 0.342
2.743GluThr: 2.743 ± 0.546
3.474GluVal: 3.474 ± 0.511
0.951GluTrp: 0.951 ± 0.173
2.048GluTyr: 2.048 ± 0.24
0.0GluXaa: 0.0 ± 0.0
Phe
3.218PheAla: 3.218 ± 0.463
1.06PheCys: 1.06 ± 0.236
1.609PheAsp: 1.609 ± 0.245
0.987PheGlu: 0.987 ± 0.229
2.048PhePhe: 2.048 ± 0.345
2.56PheGly: 2.56 ± 0.261
1.17PheHis: 1.17 ± 0.198
2.414PheIle: 2.414 ± 0.332
1.39PheLys: 1.39 ± 0.199
2.999PheLeu: 2.999 ± 0.337
1.024PheMet: 1.024 ± 0.175
1.353PheAsn: 1.353 ± 0.226
2.231PhePro: 2.231 ± 0.343
1.39PheGln: 1.39 ± 0.221
3.511PheArg: 3.511 ± 0.523
4.242PheSer: 4.242 ± 0.514
2.852PheThr: 2.852 ± 0.348
2.414PheVal: 2.414 ± 0.335
0.805PheTrp: 0.805 ± 0.153
0.914PheTyr: 0.914 ± 0.18
0.0PheXaa: 0.0 ± 0.0
Gly
4.681GlyAla: 4.681 ± 0.712
1.426GlyCys: 1.426 ± 0.32
3.84GlyAsp: 3.84 ± 0.552
4.864GlyGlu: 4.864 ± 1.072
2.706GlyPhe: 2.706 ± 0.355
4.059GlyGly: 4.059 ± 0.525
0.987GlyHis: 0.987 ± 0.212
3.73GlyIle: 3.73 ± 0.405
3.767GlyLys: 3.767 ± 0.686
4.534GlyLeu: 4.534 ± 0.344
2.633GlyMet: 2.633 ± 0.307
2.925GlyAsn: 2.925 ± 0.301
3.511GlyPro: 3.511 ± 1.533
2.377GlyGln: 2.377 ± 0.341
3.73GlyArg: 3.73 ± 0.409
3.62GlySer: 3.62 ± 0.427
3.511GlyThr: 3.511 ± 0.383
4.9GlyVal: 4.9 ± 0.407
1.097GlyTrp: 1.097 ± 0.184
2.158GlyTyr: 2.158 ± 0.303
0.0GlyXaa: 0.0 ± 0.0
His
1.682HisAla: 1.682 ± 0.261
0.768HisCys: 0.768 ± 0.181
1.024HisAsp: 1.024 ± 0.222
0.731HisGlu: 0.731 ± 0.134
1.06HisPhe: 1.06 ± 0.223
1.975HisGly: 1.975 ± 0.334
0.914HisHis: 0.914 ± 0.257
0.987HisIle: 0.987 ± 0.235
0.695HisLys: 0.695 ± 0.21
2.45HisLeu: 2.45 ± 0.575
0.475HisMet: 0.475 ± 0.13
0.951HisAsn: 0.951 ± 0.155
1.682HisPro: 1.682 ± 0.308
0.841HisGln: 0.841 ± 0.211
1.975HisArg: 1.975 ± 0.366
1.536HisSer: 1.536 ± 0.25
1.17HisThr: 1.17 ± 0.238
1.097HisVal: 1.097 ± 0.204
0.512HisTrp: 0.512 ± 0.176
0.622HisTyr: 0.622 ± 0.134
0.0HisXaa: 0.0 ± 0.0
Ile
4.169IleAla: 4.169 ± 0.354
1.06IleCys: 1.06 ± 0.243
2.925IleAsp: 2.925 ± 0.348
2.633IleGlu: 2.633 ± 0.357
1.975IlePhe: 1.975 ± 0.325
2.231IleGly: 2.231 ± 0.352
1.609IleHis: 1.609 ± 0.266
2.34IleIle: 2.34 ± 0.301
2.706IleLys: 2.706 ± 0.361
3.949IleLeu: 3.949 ± 0.585
1.024IleMet: 1.024 ± 0.166
2.669IleAsn: 2.669 ± 0.288
2.56IlePro: 2.56 ± 0.333
1.865IleGln: 1.865 ± 0.221
5.376IleArg: 5.376 ± 0.431
4.864IleSer: 4.864 ± 0.615
3.803IleThr: 3.803 ± 0.503
2.377IleVal: 2.377 ± 0.288
0.439IleTrp: 0.439 ± 0.142
1.207IleTyr: 1.207 ± 0.248
0.0IleXaa: 0.0 ± 0.0
Lys
4.9LysAla: 4.9 ± 0.501
0.475LysCys: 0.475 ± 0.144
2.487LysAsp: 2.487 ± 0.348
2.852LysGlu: 2.852 ± 0.359
0.914LysPhe: 0.914 ± 0.162
4.096LysGly: 4.096 ± 0.78
0.878LysHis: 0.878 ± 0.229
2.779LysIle: 2.779 ± 0.334
3.328LysLys: 3.328 ± 0.388
4.534LysLeu: 4.534 ± 0.36
1.463LysMet: 1.463 ± 0.245
2.889LysAsn: 2.889 ± 0.356
2.816LysPro: 2.816 ± 0.375
2.596LysGln: 2.596 ± 0.356
3.145LysArg: 3.145 ± 0.328
2.999LysSer: 2.999 ± 0.303
2.962LysThr: 2.962 ± 0.385
2.999LysVal: 2.999 ± 0.362
0.878LysTrp: 0.878 ± 0.229
1.536LysTyr: 1.536 ± 0.253
0.0LysXaa: 0.0 ± 0.0
Leu
7.314LeuAla: 7.314 ± 0.692
1.902LeuCys: 1.902 ± 0.404
2.999LeuAsp: 2.999 ± 0.344
2.925LeuGlu: 2.925 ± 0.426
3.511LeuPhe: 3.511 ± 0.48
3.108LeuGly: 3.108 ± 0.399
2.084LeuHis: 2.084 ± 0.377
4.096LeuIle: 4.096 ± 0.497
4.681LeuLys: 4.681 ± 0.472
7.57LeuLeu: 7.57 ± 0.675
2.377LeuMet: 2.377 ± 0.334
3.986LeuAsn: 3.986 ± 0.406
6.107LeuPro: 6.107 ± 0.776
3.401LeuGln: 3.401 ± 0.52
6.875LeuArg: 6.875 ± 0.742
7.57LeuSer: 7.57 ± 0.648
5.339LeuThr: 5.339 ± 0.35
5.046LeuVal: 5.046 ± 0.426
0.805LeuTrp: 0.805 ± 0.193
2.34LeuTyr: 2.34 ± 0.317
0.0LeuXaa: 0.0 ± 0.0
Met
2.633MetAla: 2.633 ± 0.304
0.366MetCys: 0.366 ± 0.116
1.024MetAsp: 1.024 ± 0.183
1.097MetGlu: 1.097 ± 0.177
0.951MetPhe: 0.951 ± 0.163
1.06MetGly: 1.06 ± 0.26
0.658MetHis: 0.658 ± 0.16
1.28MetIle: 1.28 ± 0.228
2.048MetLys: 2.048 ± 0.261
2.706MetLeu: 2.706 ± 0.347
0.841MetMet: 0.841 ± 0.194
1.426MetAsn: 1.426 ± 0.264
2.158MetPro: 2.158 ± 0.302
1.28MetGln: 1.28 ± 0.217
1.938MetArg: 1.938 ± 0.239
2.194MetSer: 2.194 ± 0.34
2.158MetThr: 2.158 ± 0.266
1.353MetVal: 1.353 ± 0.242
0.329MetTrp: 0.329 ± 0.105
0.731MetTyr: 0.731 ± 0.195
0.0MetXaa: 0.0 ± 0.0
Asn
4.023AsnAla: 4.023 ± 0.627
0.622AsnCys: 0.622 ± 0.174
1.938AsnAsp: 1.938 ± 0.306
2.743AsnGlu: 2.743 ± 0.325
1.682AsnPhe: 1.682 ± 0.265
2.889AsnGly: 2.889 ± 0.408
1.207AsnHis: 1.207 ± 0.222
2.414AsnIle: 2.414 ± 0.317
1.755AsnLys: 1.755 ± 0.287
2.999AsnLeu: 2.999 ± 0.349
1.06AsnMet: 1.06 ± 0.201
1.499AsnAsn: 1.499 ± 0.245
1.975AsnPro: 1.975 ± 0.267
1.865AsnGln: 1.865 ± 0.27
2.706AsnArg: 2.706 ± 0.372
2.999AsnSer: 2.999 ± 0.369
2.34AsnThr: 2.34 ± 0.318
2.084AsnVal: 2.084 ± 0.297
0.585AsnTrp: 0.585 ± 0.151
0.987AsnTyr: 0.987 ± 0.19
0.0AsnXaa: 0.0 ± 0.0
Pro
5.046ProAla: 5.046 ± 0.605
1.316ProCys: 1.316 ± 0.296
3.913ProAsp: 3.913 ± 0.424
4.681ProGlu: 4.681 ± 0.646
1.792ProPhe: 1.792 ± 0.251
3.181ProGly: 3.181 ± 0.419
1.134ProHis: 1.134 ± 0.282
1.28ProIle: 1.28 ± 0.256
2.194ProLys: 2.194 ± 0.444
4.388ProLeu: 4.388 ± 0.559
0.987ProMet: 0.987 ± 0.19
0.914ProAsn: 0.914 ± 0.211
2.34ProPro: 2.34 ± 0.345
2.487ProGln: 2.487 ± 0.425
2.962ProArg: 2.962 ± 0.35
3.913ProSer: 3.913 ± 0.533
1.902ProThr: 1.902 ± 0.263
5.595ProVal: 5.595 ± 0.623
0.695ProTrp: 0.695 ± 0.15
1.755ProTyr: 1.755 ± 0.285
0.0ProXaa: 0.0 ± 0.0
Gln
3.986GlnAla: 3.986 ± 0.489
0.878GlnCys: 0.878 ± 0.246
1.792GlnAsp: 1.792 ± 0.316
2.596GlnGlu: 2.596 ± 0.321
1.682GlnPhe: 1.682 ± 0.282
2.56GlnGly: 2.56 ± 0.539
1.243GlnHis: 1.243 ± 0.251
1.938GlnIle: 1.938 ± 0.294
2.816GlnLys: 2.816 ± 0.365
2.962GlnLeu: 2.962 ± 0.341
1.134GlnMet: 1.134 ± 0.226
1.975GlnAsn: 1.975 ± 0.247
2.523GlnPro: 2.523 ± 0.342
3.181GlnGln: 3.181 ± 0.641
3.949GlnArg: 3.949 ± 0.5
2.267GlnSer: 2.267 ± 0.267
1.938GlnThr: 1.938 ± 0.274
2.158GlnVal: 2.158 ± 0.355
0.841GlnTrp: 0.841 ± 0.184
1.28GlnTyr: 1.28 ± 0.217
0.0GlnXaa: 0.0 ± 0.0
Arg
4.498ArgAla: 4.498 ± 0.387
1.207ArgCys: 1.207 ± 0.281
3.364ArgAsp: 3.364 ± 0.459
4.132ArgGlu: 4.132 ± 0.513
2.999ArgPhe: 2.999 ± 0.341
5.266ArgGly: 5.266 ± 0.679
2.158ArgHis: 2.158 ± 0.314
4.352ArgIle: 4.352 ± 0.506
4.425ArgLys: 4.425 ± 0.459
6.765ArgLeu: 6.765 ± 0.517
2.669ArgMet: 2.669 ± 0.375
3.328ArgAsn: 3.328 ± 0.354
3.328ArgPro: 3.328 ± 0.439
3.949ArgGln: 3.949 ± 0.449
6.802ArgArg: 6.802 ± 0.588
5.01ArgSer: 5.01 ± 0.492
3.803ArgThr: 3.803 ± 0.452
4.059ArgVal: 4.059 ± 0.402
1.207ArgTrp: 1.207 ± 0.176
2.194ArgTyr: 2.194 ± 0.328
0.0ArgXaa: 0.0 ± 0.0
Ser
7.497SerAla: 7.497 ± 0.61
1.536SerCys: 1.536 ± 0.322
3.511SerAsp: 3.511 ± 0.403
3.218SerGlu: 3.218 ± 0.359
3.255SerPhe: 3.255 ± 0.43
6.802SerGly: 6.802 ± 0.534
0.951SerHis: 0.951 ± 0.186
3.584SerIle: 3.584 ± 0.439
2.669SerLys: 2.669 ± 0.418
6.729SerLeu: 6.729 ± 0.629
1.828SerMet: 1.828 ± 0.276
2.56SerAsn: 2.56 ± 0.309
4.315SerPro: 4.315 ± 0.509
2.706SerGln: 2.706 ± 0.292
6.034SerArg: 6.034 ± 0.67
5.778SerSer: 5.778 ± 1.014
3.84SerThr: 3.84 ± 0.393
5.156SerVal: 5.156 ± 0.501
1.024SerTrp: 1.024 ± 0.232
1.426SerTyr: 1.426 ± 0.262
0.0SerXaa: 0.0 ± 0.0
Thr
5.12ThrAla: 5.12 ± 0.418
0.951ThrCys: 0.951 ± 0.243
2.414ThrAsp: 2.414 ± 0.384
2.779ThrGlu: 2.779 ± 0.41
2.194ThrPhe: 2.194 ± 0.336
5.302ThrGly: 5.302 ± 0.59
1.426ThrHis: 1.426 ± 0.248
3.291ThrIle: 3.291 ± 0.342
2.816ThrLys: 2.816 ± 0.292
4.681ThrLeu: 4.681 ± 0.449
1.097ThrMet: 1.097 ± 0.228
1.536ThrAsn: 1.536 ± 0.277
3.62ThrPro: 3.62 ± 0.319
1.426ThrGln: 1.426 ± 0.244
2.852ThrArg: 2.852 ± 0.317
4.827ThrSer: 4.827 ± 0.468
2.925ThrThr: 2.925 ± 0.301
4.023ThrVal: 4.023 ± 0.352
0.878ThrTrp: 0.878 ± 0.171
1.097ThrTyr: 1.097 ± 0.209
0.0ThrXaa: 0.0 ± 0.0
Val
5.814ValAla: 5.814 ± 0.474
1.353ValCys: 1.353 ± 0.24
2.962ValAsp: 2.962 ± 0.407
2.377ValGlu: 2.377 ± 0.292
2.779ValPhe: 2.779 ± 0.388
2.596ValGly: 2.596 ± 0.335
1.06ValHis: 1.06 ± 0.207
3.767ValIle: 3.767 ± 0.481
2.925ValLys: 2.925 ± 0.356
6.436ValLeu: 6.436 ± 0.641
1.865ValMet: 1.865 ± 0.244
3.035ValAsn: 3.035 ± 0.307
2.925ValPro: 2.925 ± 0.333
2.231ValGln: 2.231 ± 0.267
5.558ValArg: 5.558 ± 0.666
5.229ValSer: 5.229 ± 0.426
4.352ValThr: 4.352 ± 0.425
4.169ValVal: 4.169 ± 0.441
1.024ValTrp: 1.024 ± 0.221
1.426ValTyr: 1.426 ± 0.225
0.0ValXaa: 0.0 ± 0.0
Trp
0.878TrpAla: 0.878 ± 0.196
0.293TrpCys: 0.293 ± 0.099
0.293TrpAsp: 0.293 ± 0.099
0.585TrpGlu: 0.585 ± 0.156
0.841TrpPhe: 0.841 ± 0.17
0.512TrpGly: 0.512 ± 0.132
0.366TrpHis: 0.366 ± 0.123
0.914TrpIle: 0.914 ± 0.179
0.987TrpLys: 0.987 ± 0.177
1.902TrpLeu: 1.902 ± 0.301
0.731TrpMet: 0.731 ± 0.161
0.585TrpAsn: 0.585 ± 0.163
0.512TrpPro: 0.512 ± 0.162
1.316TrpGln: 1.316 ± 0.209
1.353TrpArg: 1.353 ± 0.23
1.207TrpSer: 1.207 ± 0.196
0.585TrpThr: 0.585 ± 0.149
1.06TrpVal: 1.06 ± 0.194
0.366TrpTrp: 0.366 ± 0.115
0.329TrpTyr: 0.329 ± 0.115
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.414TyrAla: 2.414 ± 0.31
0.768TyrCys: 0.768 ± 0.21
1.28TyrAsp: 1.28 ± 0.231
0.914TyrGlu: 0.914 ± 0.204
1.353TyrPhe: 1.353 ± 0.227
1.755TyrGly: 1.755 ± 0.322
0.731TyrHis: 0.731 ± 0.165
1.463TyrIle: 1.463 ± 0.263
1.207TyrLys: 1.207 ± 0.194
1.646TyrLeu: 1.646 ± 0.256
0.658TyrMet: 0.658 ± 0.158
0.914TyrAsn: 0.914 ± 0.191
1.572TyrPro: 1.572 ± 0.249
1.207TyrGln: 1.207 ± 0.199
2.633TyrArg: 2.633 ± 0.364
1.719TyrSer: 1.719 ± 0.236
1.243TyrThr: 1.243 ± 0.269
1.426TyrVal: 1.426 ± 0.223
0.512TyrTrp: 0.512 ± 0.145
0.805TyrTyr: 0.805 ± 0.181
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 166 proteins (27347 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski