Enterobacteria phage IME10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lederbergvirus; unclassified Lederbergvirus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G5DA86|G5DA86_9CAUD ORF252 OS=Enterobacteria phage IME10 OX=1090133 PE=4 SV=1
MM1 pKa = 7.7ASMKK5 pKa = 9.68IDD7 pKa = 3.55YY8 pKa = 9.66QALRR12 pKa = 11.84EE13 pKa = 4.09KK14 pKa = 10.94AEE16 pKa = 4.07KK17 pKa = 8.76ATCGVWSLEE26 pKa = 4.05YY27 pKa = 11.02GEE29 pKa = 5.26SRR31 pKa = 11.84FDD33 pKa = 3.96GDD35 pKa = 3.49DD36 pKa = 3.43ALIHH40 pKa = 6.59RR41 pKa = 11.84EE42 pKa = 3.79VAGYY46 pKa = 10.15IPICRR51 pKa = 11.84IEE53 pKa = 4.3GAHH56 pKa = 6.67PEE58 pKa = 4.21SGFDD62 pKa = 3.18EE63 pKa = 4.95DD64 pKa = 5.53FQIEE68 pKa = 4.14QQANAEE74 pKa = 4.43FIAAANTATVLALLGEE90 pKa = 4.45LEE92 pKa = 4.32TAKK95 pKa = 10.59KK96 pKa = 10.32RR97 pKa = 11.84IAEE100 pKa = 4.18LEE102 pKa = 4.22AEE104 pKa = 4.66PVSQTCKK111 pKa = 10.62LPVNTPCQDD120 pKa = 3.39APSHH124 pKa = 6.03IWLQTAGVWPEE135 pKa = 4.02DD136 pKa = 3.88GEE138 pKa = 4.48LSEE141 pKa = 5.49LTWCSHH147 pKa = 5.17NQHH150 pKa = 6.77HH151 pKa = 8.11DD152 pKa = 3.11DD153 pKa = 3.69TLYY156 pKa = 11.06VRR158 pKa = 11.84ADD160 pKa = 3.67LVNGNSPGTPDD171 pKa = 2.86SWISCSEE178 pKa = 3.88RR179 pKa = 11.84MPDD182 pKa = 3.77SNCLYY187 pKa = 10.02LCWGTYY193 pKa = 9.72FEE195 pKa = 5.52GDD197 pKa = 3.54EE198 pKa = 4.23PDD200 pKa = 4.18YY201 pKa = 11.1IPAYY205 pKa = 10.33FFVHH209 pKa = 5.91KK210 pKa = 9.68TNGWTEE216 pKa = 3.95WQPVEE221 pKa = 5.09DD222 pKa = 4.16DD223 pKa = 3.8CNPRR227 pKa = 11.84EE228 pKa = 4.17VIITHH233 pKa = 6.41WMPLPAAPKK242 pKa = 10.22QEE244 pKa = 4.11VKK246 pKa = 10.84

Molecular weight: 27.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8EYI7|G8EYI7_9CAUD Terminase small subunit OS=Enterobacteria phage IME10 OX=1090133 GN=3 PE=4 SV=1
MM1 pKa = 7.75EE2 pKa = 5.76PRR4 pKa = 11.84TNQQLTRR11 pKa = 11.84RR12 pKa = 11.84DD13 pKa = 3.15RR14 pKa = 11.84EE15 pKa = 3.84RR16 pKa = 11.84NQRR19 pKa = 11.84RR20 pKa = 11.84GHH22 pKa = 4.2VHH24 pKa = 5.74LWSYY28 pKa = 11.44ASSEE32 pKa = 4.33TTQICQPDD40 pKa = 4.01VYY42 pKa = 10.13RR43 pKa = 11.84YY44 pKa = 9.56RR45 pKa = 11.84SSVLRR50 pKa = 11.84KK51 pKa = 8.29PHH53 pKa = 6.63IDD55 pKa = 3.06SGTGRR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84MRR64 pKa = 11.84LEE66 pKa = 3.91SVAKK70 pKa = 9.15FHH72 pKa = 6.87SPKK75 pKa = 10.44SPMMSDD81 pKa = 3.41SPRR84 pKa = 11.84ATASDD89 pKa = 4.01SLSGTDD95 pKa = 3.38VMAAMGMAQSQAGFGMAAFCGKK117 pKa = 9.93HH118 pKa = 5.73EE119 pKa = 4.72LSQNDD124 pKa = 3.28KK125 pKa = 10.89QKK127 pKa = 11.16AINYY131 pKa = 8.5LMQFAHH137 pKa = 6.59KK138 pKa = 10.66VSGKK142 pKa = 9.17YY143 pKa = 9.64RR144 pKa = 11.84GVAKK148 pKa = 10.96LEE150 pKa = 4.3GNTKK154 pKa = 10.64AKK156 pKa = 10.08VLQVLATFAYY166 pKa = 10.49ADD168 pKa = 3.87YY169 pKa = 9.9CRR171 pKa = 11.84SAATPGARR179 pKa = 11.84CRR181 pKa = 11.84DD182 pKa = 3.55CHH184 pKa = 5.14GTGRR188 pKa = 11.84AVDD191 pKa = 3.51IAKK194 pKa = 8.9TEE196 pKa = 3.51QWGRR200 pKa = 11.84VVEE203 pKa = 4.53KK204 pKa = 10.74EE205 pKa = 3.88CGRR208 pKa = 11.84CKK210 pKa = 10.51GVGYY214 pKa = 10.85SRR216 pKa = 11.84MPASAAYY223 pKa = 9.71RR224 pKa = 11.84AVTMLIPNLTQPTWSRR240 pKa = 11.84TVKK243 pKa = 10.04PLYY246 pKa = 10.17DD247 pKa = 3.46ALVVQCHH254 pKa = 5.63KK255 pKa = 10.78EE256 pKa = 3.65EE257 pKa = 4.83SIADD261 pKa = 3.94NILNAVTRR269 pKa = 4.15

Molecular weight: 29.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27

0

27

7427

107

721

275.1

30.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.465 ± 0.655

1.118 ± 0.177

6.247 ± 0.265

7.136 ± 0.747

2.976 ± 0.231

6.907 ± 0.424

1.616 ± 0.169

5.695 ± 0.271

6.14 ± 0.341

7.54 ± 0.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.895 ± 0.188

4.753 ± 0.344

3.932 ± 0.297

5.426 ± 0.467

6.099 ± 0.381

6.019 ± 0.417

5.17 ± 0.331

5.924 ± 0.456

1.548 ± 0.213

3.393 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski