Clostridium sp. CAG:533

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 969 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6AMM8|R6AMM8_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:533 OX=1262818 GN=BN698_00149 PE=4 SV=1
MM1 pKa = 7.35CTLNNRR7 pKa = 11.84TITYY11 pKa = 10.16SFEE14 pKa = 3.75EE15 pKa = 5.4DD16 pKa = 3.01ILKK19 pKa = 10.47SVHH22 pKa = 7.44DD23 pKa = 4.01NVTNNDD29 pKa = 3.09TSDD32 pKa = 2.9IDD34 pKa = 4.5AYY36 pKa = 10.27MALLGKK42 pKa = 9.52YY43 pKa = 8.87QEE45 pKa = 4.16LSNRR49 pKa = 11.84LVSNGLVSTTEE60 pKa = 3.93EE61 pKa = 4.17TDD63 pKa = 3.15SGFAFNADD71 pKa = 3.27VPLSEE76 pKa = 5.52NIRR79 pKa = 11.84EE80 pKa = 4.33TLGEE84 pKa = 4.4DD85 pKa = 3.1YY86 pKa = 10.63DD87 pKa = 4.13YY88 pKa = 11.65NYY90 pKa = 10.8FYY92 pKa = 10.99MNAPLKK98 pKa = 9.87EE99 pKa = 4.0VSYY102 pKa = 11.98DD103 pKa = 3.45MLTKK107 pKa = 10.89GFDD110 pKa = 3.6CKK112 pKa = 11.08

Molecular weight: 12.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6APL9|R6APL9_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:533 OX=1262818 GN=BN698_00428 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 8.28MKK13 pKa = 10.36KK14 pKa = 9.78KK15 pKa = 10.35FGFFARR21 pKa = 11.84KK22 pKa = 9.27ASNILNRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84AKK33 pKa = 9.82GRR35 pKa = 11.84KK36 pKa = 8.16VLCKK40 pKa = 10.47

Molecular weight: 4.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

969

0

969

273910

33

1534

282.7

32.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.911 ± 0.07

1.115 ± 0.031

6.049 ± 0.069

7.181 ± 0.093

4.203 ± 0.065

5.671 ± 0.076

1.253 ± 0.028

9.602 ± 0.107

9.785 ± 0.084

9.275 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.381 ± 0.036

7.075 ± 0.077

2.543 ± 0.038

1.733 ± 0.033

3.036 ± 0.054

6.505 ± 0.07

5.754 ± 0.083

6.248 ± 0.076

0.523 ± 0.024

5.158 ± 0.067
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski