Rice stripe necrosis virus 
Average proteome isoelectric point is 6.49 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|B5STA0|B5STA0_9VIRU 12K protein OS=Rice stripe necrosis virus OX=373373 GN=TGBp2 PE=4 SV=1 
MM1 pKa = 6.36  TSEE4 pKa = 4.24  WARR7 pKa = 11.84  EE8 pKa = 3.87  HH9 pKa = 6.1  PTDD12 pKa = 3.23  VFAIFEE18 pKa = 4.32  TCARR22 pKa = 11.84  EE23 pKa = 5.74  AGFTWNDD30 pKa = 3.35  VPPHH34 pKa = 5.41  VVNFDD39 pKa = 3.57  SLEE42 pKa = 3.92  KK43 pKa = 10.78  SKK45 pKa = 11.05  AISNLTDD52 pKa = 3.66  LLEE55 pKa = 4.68  NEE57 pKa = 4.36  VAKK60 pKa = 10.78  GCKK63 pKa = 9.46  EE64 pKa = 4.09  KK65 pKa = 11.28  GDD67 pKa = 3.51  VAAIKK72 pKa = 10.55  LEE74 pKa = 4.1  NVNGMTKK81 pKa = 10.07  EE82 pKa = 3.69  YY83 pKa = 9.19  NARR86 pKa = 11.84  VGVCVGAPGAGKK98 pKa = 6.85  TTLIKK103 pKa = 10.74  AVMSKK108 pKa = 10.73  ASRR111 pKa = 11.84  VVIAVPNSTLLKK123 pKa = 10.21  NVYY126 pKa = 9.84  SGNPNAFLIDD136 pKa = 4.0  DD137 pKa = 4.58  LFSRR141 pKa = 11.84  PVEE144 pKa = 3.78  FAKK147 pKa = 11.17  YY148 pKa = 7.38  EE149 pKa = 4.16  TILIDD154 pKa = 3.79  EE155 pKa = 4.47  FTKK158 pKa = 10.54  VHH160 pKa = 4.94  VCEE163 pKa = 4.07  VLMLSALLRR172 pKa = 11.84  VKK174 pKa = 10.51  NILMFGDD181 pKa = 4.33  PQQGMHH187 pKa = 6.0  YY188 pKa = 9.81  RR189 pKa = 11.84  PGSFVYY195 pKa = 10.97  YY196 pKa = 9.8  NFPVLAEE203 pKa = 4.01  SHH205 pKa = 6.52  ASHH208 pKa = 7.1  RR209 pKa = 11.84  MPQAIGEE216 pKa = 4.36  AYY218 pKa = 9.83  NNAMGTKK225 pKa = 9.21  IEE227 pKa = 4.48  PKK229 pKa = 9.86  SSQSGEE235 pKa = 4.1  FEE237 pKa = 4.15  IKK239 pKa = 10.47  DD240 pKa = 3.97  LLGMIRR246 pKa = 11.84  DD247 pKa = 3.83  KK248 pKa = 11.64  SKK250 pKa = 10.92  VLCLSEE256 pKa = 4.19  KK257 pKa = 9.2  TQSNLDD263 pKa = 3.5  DD264 pKa = 4.77  CGISAEE270 pKa = 4.54  LVSKK274 pKa = 10.66  VQGCEE279 pKa = 3.67  FAAVTLILEE288 pKa = 4.65  EE289 pKa = 4.48  PQDD292 pKa = 3.63  IAPFCNKK299 pKa = 10.25  SIRR302 pKa = 11.84  CVALSRR308 pKa = 11.84  AKK310 pKa = 10.18  EE311 pKa = 3.9  VLIIQATPYY320 pKa = 9.35  FKK322 pKa = 11.33  SMLCNAEE329 pKa = 4.13  FVDD332 pKa = 4.74  PYY334 pKa = 10.87  EE335 pKa = 4.4  VDD337 pKa = 3.74  SSCSGQTLCNSRR349 pKa = 3.74   
 Molecular weight: 38.42 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  5.294 
IPC2_protein 5.41 
IPC_protein 5.372 
Toseland    5.423 
ProMoST     5.563 
Dawson      5.436 
Bjellqvist  5.525 
Wikipedia   5.334 
Rodwell     5.385 
Grimsley    5.397 
Solomon     5.436 
Lehninger   5.397 
Nozaki      5.601 
DTASelect   5.728 
Thurlkill   5.537 
EMBOSS      5.474 
Sillero     5.69 
Patrickios  3.961 
IPC_peptide 5.448 
IPC2_peptide  5.677 
IPC2.peptide.svr19  5.711 
 Protein with the highest isoelectric point: 
>tr|B5STA1|B5STA1_9VIRU 15K protein OS=Rice stripe necrosis virus OX=373373 GN=TGBp3 PE=4 SV=2 
MM1 pKa = 7.35  SGQYY5 pKa = 10.44  VSARR9 pKa = 11.84  PNKK12 pKa = 9.89  FIPICICIGVVCVAVCLVLATPRR35 pKa = 11.84  HH36 pKa = 5.19  KK37 pKa = 9.4  THH39 pKa = 6.51  SAGDD43 pKa = 3.61  YY44 pKa = 9.83  GVPTFANGGSYY55 pKa = 11.33  ADD57 pKa = 3.45  GTRR60 pKa = 11.84  RR61 pKa = 11.84  AKK63 pKa = 10.46  FNCNNDD69 pKa = 3.2  RR70 pKa = 11.84  AYY72 pKa = 10.64  GSSQPQMSSNFVAFIAVILLVAFALRR98 pKa = 11.84  SCNSVGNCGEE108 pKa = 4.11  RR109 pKa = 11.84  CNGGCCKK116 pKa = 10.55  NN117 pKa = 3.46   
 Molecular weight: 12.36 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.053 
IPC2_protein 8.024 
IPC_protein 7.863 
Toseland    7.263 
ProMoST     8.419 
Dawson      8.507 
Bjellqvist  9.033 
Wikipedia   8.419 
Rodwell     8.521 
Grimsley    7.205 
Solomon     8.609 
Lehninger   8.639 
Nozaki      9.355 
DTASelect   8.653 
Thurlkill   8.697 
EMBOSS      8.726 
Sillero     9.092 
Patrickios  0.846 
IPC_peptide 8.609 
IPC2_peptide  8.77 
IPC2.peptide.svr19  8.919 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        8 
 
        
        0
 
        
        8 
         
        4177
 
        117
 
        2100
 
        522.1
 
        58.27
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        8.283 ± 0.225
1.819 ± 0.648
 
        5.602 ± 0.276
6.416 ± 0.581
 
        4.166 ± 0.302
6.584 ± 0.538
 
        1.891 ± 0.202
4.956 ± 0.306
       
        4.812 ± 0.534
8.188 ± 0.432
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.657 ± 0.293
4.597 ± 0.282
 
        4.98 ± 0.447
3.663 ± 0.41
 
        6.512 ± 0.743
7.709 ± 0.679
 
        4.74 ± 0.237
7.541 ± 0.831
       
        1.269 ± 0.241
3.543 ± 0.258
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here