Rice stripe necrosis virus
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B5STA0|B5STA0_9VIRU 12K protein OS=Rice stripe necrosis virus OX=373373 GN=TGBp2 PE=4 SV=1
MM1 pKa = 6.36 TSEE4 pKa = 4.24 WARR7 pKa = 11.84 EE8 pKa = 3.87 HH9 pKa = 6.1 PTDD12 pKa = 3.23 VFAIFEE18 pKa = 4.32 TCARR22 pKa = 11.84 EE23 pKa = 5.74 AGFTWNDD30 pKa = 3.35 VPPHH34 pKa = 5.41 VVNFDD39 pKa = 3.57 SLEE42 pKa = 3.92 KK43 pKa = 10.78 SKK45 pKa = 11.05 AISNLTDD52 pKa = 3.66 LLEE55 pKa = 4.68 NEE57 pKa = 4.36 VAKK60 pKa = 10.78 GCKK63 pKa = 9.46 EE64 pKa = 4.09 KK65 pKa = 11.28 GDD67 pKa = 3.51 VAAIKK72 pKa = 10.55 LEE74 pKa = 4.1 NVNGMTKK81 pKa = 10.07 EE82 pKa = 3.69 YY83 pKa = 9.19 NARR86 pKa = 11.84 VGVCVGAPGAGKK98 pKa = 6.85 TTLIKK103 pKa = 10.74 AVMSKK108 pKa = 10.73 ASRR111 pKa = 11.84 VVIAVPNSTLLKK123 pKa = 10.21 NVYY126 pKa = 9.84 SGNPNAFLIDD136 pKa = 4.0 DD137 pKa = 4.58 LFSRR141 pKa = 11.84 PVEE144 pKa = 3.78 FAKK147 pKa = 11.17 YY148 pKa = 7.38 EE149 pKa = 4.16 TILIDD154 pKa = 3.79 EE155 pKa = 4.47 FTKK158 pKa = 10.54 VHH160 pKa = 4.94 VCEE163 pKa = 4.07 VLMLSALLRR172 pKa = 11.84 VKK174 pKa = 10.51 NILMFGDD181 pKa = 4.33 PQQGMHH187 pKa = 6.0 YY188 pKa = 9.81 RR189 pKa = 11.84 PGSFVYY195 pKa = 10.97 YY196 pKa = 9.8 NFPVLAEE203 pKa = 4.01 SHH205 pKa = 6.52 ASHH208 pKa = 7.1 RR209 pKa = 11.84 MPQAIGEE216 pKa = 4.36 AYY218 pKa = 9.83 NNAMGTKK225 pKa = 9.21 IEE227 pKa = 4.48 PKK229 pKa = 9.86 SSQSGEE235 pKa = 4.1 FEE237 pKa = 4.15 IKK239 pKa = 10.47 DD240 pKa = 3.97 LLGMIRR246 pKa = 11.84 DD247 pKa = 3.83 KK248 pKa = 11.64 SKK250 pKa = 10.92 VLCLSEE256 pKa = 4.19 KK257 pKa = 9.2 TQSNLDD263 pKa = 3.5 DD264 pKa = 4.77 CGISAEE270 pKa = 4.54 LVSKK274 pKa = 10.66 VQGCEE279 pKa = 3.67 FAAVTLILEE288 pKa = 4.65 EE289 pKa = 4.48 PQDD292 pKa = 3.63 IAPFCNKK299 pKa = 10.25 SIRR302 pKa = 11.84 CVALSRR308 pKa = 11.84 AKK310 pKa = 10.18 EE311 pKa = 3.9 VLIIQATPYY320 pKa = 9.35 FKK322 pKa = 11.33 SMLCNAEE329 pKa = 4.13 FVDD332 pKa = 4.74 PYY334 pKa = 10.87 EE335 pKa = 4.4 VDD337 pKa = 3.74 SSCSGQTLCNSRR349 pKa = 3.74
Molecular weight: 38.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.294
IPC2_protein 5.41
IPC_protein 5.372
Toseland 5.423
ProMoST 5.563
Dawson 5.436
Bjellqvist 5.525
Wikipedia 5.334
Rodwell 5.385
Grimsley 5.397
Solomon 5.436
Lehninger 5.397
Nozaki 5.601
DTASelect 5.728
Thurlkill 5.537
EMBOSS 5.474
Sillero 5.69
Patrickios 3.961
IPC_peptide 5.448
IPC2_peptide 5.677
IPC2.peptide.svr19 5.711
Protein with the highest isoelectric point:
>tr|B5STA1|B5STA1_9VIRU 15K protein OS=Rice stripe necrosis virus OX=373373 GN=TGBp3 PE=4 SV=2
MM1 pKa = 7.35 SGQYY5 pKa = 10.44 VSARR9 pKa = 11.84 PNKK12 pKa = 9.89 FIPICICIGVVCVAVCLVLATPRR35 pKa = 11.84 HH36 pKa = 5.19 KK37 pKa = 9.4 THH39 pKa = 6.51 SAGDD43 pKa = 3.61 YY44 pKa = 9.83 GVPTFANGGSYY55 pKa = 11.33 ADD57 pKa = 3.45 GTRR60 pKa = 11.84 RR61 pKa = 11.84 AKK63 pKa = 10.46 FNCNNDD69 pKa = 3.2 RR70 pKa = 11.84 AYY72 pKa = 10.64 GSSQPQMSSNFVAFIAVILLVAFALRR98 pKa = 11.84 SCNSVGNCGEE108 pKa = 4.11 RR109 pKa = 11.84 CNGGCCKK116 pKa = 10.55 NN117 pKa = 3.46
Molecular weight: 12.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.053
IPC2_protein 8.024
IPC_protein 7.863
Toseland 7.263
ProMoST 8.419
Dawson 8.507
Bjellqvist 9.033
Wikipedia 8.419
Rodwell 8.521
Grimsley 7.205
Solomon 8.609
Lehninger 8.639
Nozaki 9.355
DTASelect 8.653
Thurlkill 8.697
EMBOSS 8.726
Sillero 9.092
Patrickios 0.846
IPC_peptide 8.609
IPC2_peptide 8.77
IPC2.peptide.svr19 8.919
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
4177
117
2100
522.1
58.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.283 ± 0.225
1.819 ± 0.648
5.602 ± 0.276
6.416 ± 0.581
4.166 ± 0.302
6.584 ± 0.538
1.891 ± 0.202
4.956 ± 0.306
4.812 ± 0.534
8.188 ± 0.432
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.657 ± 0.293
4.597 ± 0.282
4.98 ± 0.447
3.663 ± 0.41
6.512 ± 0.743
7.709 ± 0.679
4.74 ± 0.237
7.541 ± 0.831
1.269 ± 0.241
3.543 ± 0.258
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here