Campylobacter sputorum biovar sputorum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Campylobacteraceae; Campylobacter; Campylobacter sputorum

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1764 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A381DJ02|A0A381DJ02_9PROT Putative fibronectin/fibrinogen-binding protein OS=Campylobacter sputorum biovar sputorum OX=32024 GN=NCTC12475_00787 PE=4 SV=1
MM1 pKa = 7.43AVKK4 pKa = 9.0ITDD7 pKa = 3.0ICIACGSCIDD17 pKa = 3.95EE18 pKa = 4.91CPVEE22 pKa = 5.18AIVDD26 pKa = 3.73DD27 pKa = 3.9SDD29 pKa = 3.69NPKK32 pKa = 10.92GEE34 pKa = 4.92DD35 pKa = 2.84IYY37 pKa = 11.63YY38 pKa = 10.05VYY40 pKa = 10.18PDD42 pKa = 3.59KK43 pKa = 11.21CVEE46 pKa = 4.3CVGHH50 pKa = 6.75NDD52 pKa = 3.57QPACASACPTDD63 pKa = 4.4GCIVWDD69 pKa = 4.51APVDD73 pKa = 4.04GQPSRR78 pKa = 11.84SEE80 pKa = 3.49ISADD84 pKa = 3.22MRR86 pKa = 11.84NGSTPVVQQ94 pKa = 4.38

Molecular weight: 9.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A381DIN2|A0A381DIN2_9PROT Flagellar M-ring protein OS=Campylobacter sputorum biovar sputorum OX=32024 GN=fliF PE=3 SV=1
MM1 pKa = 7.28KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 9.99QPHH8 pKa = 6.42KK9 pKa = 9.25LPRR12 pKa = 11.84VRR14 pKa = 11.84THH16 pKa = 6.14GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.97TKK25 pKa = 10.39NGRR28 pKa = 11.84KK29 pKa = 9.12IINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.51GRR39 pKa = 11.84KK40 pKa = 8.68RR41 pKa = 11.84LSVV44 pKa = 3.2

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1764

0

1764

538754

33

2613

305.4

34.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.069 ± 0.055

1.192 ± 0.027

5.947 ± 0.062

6.195 ± 0.059

5.641 ± 0.063

5.864 ± 0.067

1.463 ± 0.021

9.805 ± 0.069

9.547 ± 0.064

9.453 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.509 ± 0.024

6.77 ± 0.077

2.687 ± 0.034

2.622 ± 0.031

2.874 ± 0.034

6.891 ± 0.043

4.421 ± 0.035

5.556 ± 0.054

0.643 ± 0.018

3.851 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski