Escherichia virus LS2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Kayfunavirus; unclassified Kayfunavirus

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D5FUK9|A0A7D5FUK9_9CAUD Primase OS=Escherichia virus LS2 OX=2743776 GN=LS2_21 PE=3 SV=1
MM1 pKa = 7.37EE2 pKa = 5.49RR3 pKa = 11.84NANAYY8 pKa = 10.17YY9 pKa = 10.19EE10 pKa = 4.4LLAATVEE17 pKa = 4.13AFIEE21 pKa = 4.8RR22 pKa = 11.84IQYY25 pKa = 10.64DD26 pKa = 4.39HH27 pKa = 6.98LTQDD31 pKa = 4.22DD32 pKa = 4.81DD33 pKa = 4.96YY34 pKa = 11.8SDD36 pKa = 4.23ALHH39 pKa = 6.46EE40 pKa = 4.42VVGDD44 pKa = 3.81QVPHH48 pKa = 6.36YY49 pKa = 8.69YY50 pKa = 10.4HH51 pKa = 7.5EE52 pKa = 4.81IFTVMAADD60 pKa = 5.4GIDD63 pKa = 3.84HH64 pKa = 6.56EE65 pKa = 5.23FEE67 pKa = 5.3DD68 pKa = 4.36SGLMPEE74 pKa = 4.55TKK76 pKa = 10.2DD77 pKa = 3.18VTRR80 pKa = 11.84ILQARR85 pKa = 11.84IYY87 pKa = 8.84EE88 pKa = 4.05ALYY91 pKa = 10.81NDD93 pKa = 3.8VSNSLDD99 pKa = 3.62VVWFEE104 pKa = 5.44DD105 pKa = 3.95EE106 pKa = 4.14EE107 pKa = 5.87ADD109 pKa = 5.03EE110 pKa = 4.35EE111 pKa = 4.27DD112 pKa = 4.56EE113 pKa = 4.47YY114 pKa = 11.18WVVDD118 pKa = 4.07AKK120 pKa = 10.28TGAIIVQAVALEE132 pKa = 4.19VATACAKK139 pKa = 10.32VNYY142 pKa = 9.74AAGRR146 pKa = 11.84HH147 pKa = 5.33LKK149 pKa = 10.6VEE151 pKa = 4.94DD152 pKa = 3.52INDD155 pKa = 3.43NVVFDD160 pKa = 4.99PAAEE164 pKa = 4.18DD165 pKa = 4.0CEE167 pKa = 4.3RR168 pKa = 5.01

Molecular weight: 19.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D5FT26|A0A7D5FT26_9CAUD Uncharacterized protein OS=Escherichia virus LS2 OX=2743776 GN=LS2_40 PE=4 SV=1
MM1 pKa = 7.41LRR3 pKa = 11.84KK4 pKa = 10.15LKK6 pKa = 10.55ARR8 pKa = 11.84YY9 pKa = 8.73HH10 pKa = 5.97RR11 pKa = 11.84FMYY14 pKa = 9.78KK15 pKa = 8.07WWSDD19 pKa = 3.14EE20 pKa = 4.0ATCLSNILGDD30 pKa = 3.61QRR32 pKa = 11.84FDD34 pKa = 3.17SKK36 pKa = 10.92AWKK39 pKa = 9.15RR40 pKa = 11.84ANRR43 pKa = 11.84KK44 pKa = 8.77FMYY47 pKa = 10.17HH48 pKa = 6.57FLRR51 pKa = 11.84TDD53 pKa = 4.04FF54 pKa = 4.64

Molecular weight: 6.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

11375

31

1339

258.5

28.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.492 ± 0.383

1.108 ± 0.15

6.435 ± 0.27

6.224 ± 0.381

3.596 ± 0.183

6.954 ± 0.39

1.574 ± 0.185

5.081 ± 0.296

6.136 ± 0.358

8.527 ± 0.434

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.059 ± 0.217

4.484 ± 0.265

3.64 ± 0.191

3.64 ± 0.452

5.846 ± 0.325

6.629 ± 0.378

6.347 ± 0.339

7.235 ± 0.355

1.503 ± 0.158

3.49 ± 0.198
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski