Staphylococcus virus 52a

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Phietavirus

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4ZAR7|Q4ZAR7_9CAUD ORF033 OS=Staphylococcus virus 52a OX=320847 PE=4 SV=1
MM1 pKa = 6.89QQQAYY6 pKa = 9.38INATIDD12 pKa = 2.95IRR14 pKa = 11.84IPTEE18 pKa = 3.74VEE20 pKa = 3.68YY21 pKa = 10.96HH22 pKa = 6.61HH23 pKa = 7.08FDD25 pKa = 4.33DD26 pKa = 3.91VDD28 pKa = 3.54KK29 pKa = 10.81EE30 pKa = 4.39KK31 pKa = 11.06EE32 pKa = 4.04VLADD36 pKa = 3.49YY37 pKa = 10.72LYY39 pKa = 11.16NNPDD43 pKa = 3.71EE44 pKa = 4.28ILEE47 pKa = 3.92YY48 pKa = 11.25DD49 pKa = 3.43NLKK52 pKa = 9.69IRR54 pKa = 11.84NVNVEE59 pKa = 3.93VEE61 pKa = 4.1

Molecular weight: 7.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q4ZAT0|Q4ZAT0_9CAUD ORF079 OS=Staphylococcus virus 52a OX=320847 PE=4 SV=1
MM1 pKa = 7.2NVAILANPAIDD12 pKa = 3.52HH13 pKa = 6.54AVVKK17 pKa = 9.88YY18 pKa = 10.11RR19 pKa = 11.84NGMSTIAKK27 pKa = 8.97IVKK30 pKa = 9.82HH31 pKa = 5.0STANRR36 pKa = 3.51

Molecular weight: 3.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

13118

35

1047

201.8

23.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.794 ± 0.362

0.549 ± 0.099

6.038 ± 0.304

7.494 ± 0.398

4.322 ± 0.25

5.916 ± 0.32

1.845 ± 0.134

7.311 ± 0.282

9.148 ± 0.334

7.333 ± 0.211

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.592 ± 0.196

6.602 ± 0.28

2.798 ± 0.219

4.353 ± 0.211

4.025 ± 0.21

5.755 ± 0.26

6.289 ± 0.256

6.266 ± 0.251

1.22 ± 0.161

4.353 ± 0.339
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski