Cryptosporidium parvum virus 1 (strain KSU-1)
Average proteome isoelectric point is 7.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|O15926|CAPSD_CPVKS Capsid protein OS=Cryptosporidium parvum virus 1 (strain KSU-1) OX=766191 PE=3 SV=1
MM1 pKa = 6.86 ITSFEE6 pKa = 4.33 SIEE9 pKa = 4.24 KK10 pKa = 10.25 KK11 pKa = 10.13 NAVYY15 pKa = 10.85 YY16 pKa = 10.09 PVDD19 pKa = 3.83 LKK21 pKa = 11.19 FVTDD25 pKa = 4.48 LSSDD29 pKa = 3.75 LSNTADD35 pKa = 3.45 GLGQAWYY42 pKa = 9.68 KK43 pKa = 9.96 ISQVAVEE50 pKa = 4.83 HH51 pKa = 6.98 IILTALKK58 pKa = 9.58 INYY61 pKa = 8.35 VLNHH65 pKa = 5.94 RR66 pKa = 11.84 TLLLIYY72 pKa = 9.68 NKK74 pKa = 9.91 KK75 pKa = 9.59 VPDD78 pKa = 3.55 MDD80 pKa = 5.81 LINIIQSSIMVPRR93 pKa = 11.84 FVRR96 pKa = 11.84 DD97 pKa = 4.0 LIRR100 pKa = 11.84 EE101 pKa = 4.07 ILRR104 pKa = 11.84 PMHH107 pKa = 6.58 HH108 pKa = 6.69 SGITYY113 pKa = 10.0 IPDD116 pKa = 3.93 LDD118 pKa = 4.3 LSTRR122 pKa = 11.84 PTPHH126 pKa = 7.38 LLDD129 pKa = 3.31 TFYY132 pKa = 10.66 PIAEE136 pKa = 4.42 HH137 pKa = 5.41 LTRR140 pKa = 11.84 WNNVCQKK147 pKa = 10.48 LGFEE151 pKa = 4.64 MVPILPEE158 pKa = 3.98 AVQSVSLTFYY168 pKa = 10.79 SYY170 pKa = 10.01 EE171 pKa = 4.0 TDD173 pKa = 3.19 EE174 pKa = 5.57 LLSFDD179 pKa = 4.89 NLINLDD185 pKa = 3.26 WRR187 pKa = 11.84 IEE189 pKa = 3.93 AFGWTKK195 pKa = 10.68 HH196 pKa = 5.83 LVHH199 pKa = 6.7 NPTSEE204 pKa = 3.74 VDD206 pKa = 3.65 EE207 pKa = 4.81 LGKK210 pKa = 10.41 SQTATSRR217 pKa = 11.84 KK218 pKa = 8.62 RR219 pKa = 11.84 VQEE222 pKa = 3.74 KK223 pKa = 10.46 DD224 pKa = 3.55 YY225 pKa = 11.36 EE226 pKa = 4.83 CDD228 pKa = 3.38 DD229 pKa = 3.47 FRR231 pKa = 11.84 KK232 pKa = 10.17 VYY234 pKa = 9.74 EE235 pKa = 4.31 RR236 pKa = 11.84 PLQNRR241 pKa = 11.84 RR242 pKa = 11.84 ILGMIVYY249 pKa = 9.77 RR250 pKa = 11.84 YY251 pKa = 7.17 TCAPYY256 pKa = 10.54 TMRR259 pKa = 11.84 LGHH262 pKa = 7.15 LSPNCRR268 pKa = 11.84 FPTEE272 pKa = 4.38 KK273 pKa = 10.25 NHH275 pKa = 6.78 SEE277 pKa = 4.15 TPPTSNRR284 pKa = 11.84 VLLSEE289 pKa = 3.85 QTMVHH294 pKa = 6.68 SIQKK298 pKa = 9.38 NRR300 pKa = 11.84 SKK302 pKa = 10.45 PKK304 pKa = 8.99 KK305 pKa = 8.57 VKK307 pKa = 10.18 IVTTEE312 pKa = 3.97 CSADD316 pKa = 3.57 RR317 pKa = 11.84 SHH319 pKa = 7.26
Molecular weight: 37.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.02
IPC2_protein 7.307
IPC_protein 7.337
Toseland 7.19
ProMoST 7.702
Dawson 7.966
Bjellqvist 8.17
Wikipedia 7.907
Rodwell 7.966
Grimsley 7.293
Solomon 8.083
Lehninger 8.097
Nozaki 8.346
DTASelect 8.083
Thurlkill 8.126
EMBOSS 8.214
Sillero 8.39
Patrickios 4.291
IPC_peptide 8.068
IPC2_peptide 7.395
IPC2.peptide.svr19 7.402
Protein with the highest isoelectric point:
>sp|O15926|CAPSD_CPVKS Capsid protein OS=Cryptosporidium parvum virus 1 (strain KSU-1) OX=766191 PE=3 SV=1
MM1 pKa = 7.41 KK2 pKa = 10.09 FVNIYY7 pKa = 9.6 EE8 pKa = 4.09 IQRR11 pKa = 11.84 FDD13 pKa = 3.65 GQPTRR18 pKa = 11.84 HH19 pKa = 6.29 GIAPKK24 pKa = 10.24 KK25 pKa = 9.93 IFRR28 pKa = 11.84 SKK30 pKa = 10.75 YY31 pKa = 8.63 IPTGLVPRR39 pKa = 11.84 LKK41 pKa = 10.01 YY42 pKa = 9.66 WRR44 pKa = 11.84 DD45 pKa = 3.29 VPSRR49 pKa = 11.84 AEE51 pKa = 3.55 MSKK54 pKa = 10.65 RR55 pKa = 11.84 IGKK58 pKa = 9.31 YY59 pKa = 9.85 FEE61 pKa = 5.87 DD62 pKa = 3.71 EE63 pKa = 4.3 FKK65 pKa = 10.67 FYY67 pKa = 10.61 PNEE70 pKa = 3.92 EE71 pKa = 4.29 KK72 pKa = 11.02 LNEE75 pKa = 4.07 AVNIVQEE82 pKa = 4.26 KK83 pKa = 8.98 WISHH87 pKa = 6.06 YY88 pKa = 9.49 GTSLNVTSVSEE99 pKa = 4.33 SFRR102 pKa = 11.84 TLPKK106 pKa = 9.45 STSAGLPFKK115 pKa = 10.71 SGCTKK120 pKa = 10.57 YY121 pKa = 9.93 EE122 pKa = 3.75 ARR124 pKa = 11.84 NKK126 pKa = 9.12 MMRR129 pKa = 11.84 FARR132 pKa = 11.84 SQWDD136 pKa = 3.57 RR137 pKa = 11.84 VSKK140 pKa = 10.2 EE141 pKa = 3.64 LQLQVLPCRR150 pKa = 11.84 LGARR154 pKa = 11.84 CQLRR158 pKa = 11.84 KK159 pKa = 9.98 RR160 pKa = 11.84 GEE162 pKa = 4.02 NKK164 pKa = 9.47 PRR166 pKa = 11.84 LIWAYY171 pKa = 9.41 PGYY174 pKa = 10.83 LSIIEE179 pKa = 4.16 NQYY182 pKa = 8.77 LTAIKK187 pKa = 9.95 KK188 pKa = 8.94 VPPPNFIGWSTNWLDD203 pKa = 3.94 GGKK206 pKa = 9.65 SLNRR210 pKa = 11.84 LLFGDD215 pKa = 3.26 KK216 pKa = 8.38 WTWQSIAQIDD226 pKa = 4.29 FSSFDD231 pKa = 3.15 ATVRR235 pKa = 11.84 TEE237 pKa = 4.68 LIFHH241 pKa = 7.16 AFKK244 pKa = 10.69 ILRR247 pKa = 11.84 SLFDD251 pKa = 3.16 LTRR254 pKa = 11.84 TEE256 pKa = 5.79 NIMLDD261 pKa = 3.47 QLRR264 pKa = 11.84 HH265 pKa = 4.97 YY266 pKa = 9.77 FINTPILFYY275 pKa = 11.29 DD276 pKa = 4.11 KK277 pKa = 10.58 IIVKK281 pKa = 9.98 NRR283 pKa = 11.84 GIPSGSAFTQIIGTIVNMIACQYY306 pKa = 10.82 ASLRR310 pKa = 11.84 SRR312 pKa = 11.84 DD313 pKa = 3.61 YY314 pKa = 11.0 NLRR317 pKa = 11.84 IPFSCWLGDD326 pKa = 3.55 DD327 pKa = 3.76 SFLNFEE333 pKa = 4.29 TALCRR338 pKa = 11.84 QEE340 pKa = 5.24 FEE342 pKa = 3.84 YY343 pKa = 10.73 DD344 pKa = 3.42 YY345 pKa = 11.59 LEE347 pKa = 4.26 KK348 pKa = 10.76 FKK350 pKa = 11.04 EE351 pKa = 3.91 LGLNVSIDD359 pKa = 3.34 KK360 pKa = 9.0 THH362 pKa = 5.42 YY363 pKa = 4.91 TTRR366 pKa = 11.84 FIDD369 pKa = 3.83 DD370 pKa = 3.52 FEE372 pKa = 4.74 VRR374 pKa = 11.84 FKK376 pKa = 11.06 GVRR379 pKa = 11.84 PYY381 pKa = 11.53 VKK383 pKa = 10.34 FLGKK387 pKa = 10.44 QIDD390 pKa = 3.6 ILLDD394 pKa = 3.52 LTFHH398 pKa = 7.21 NDD400 pKa = 3.13 LDD402 pKa = 4.16 KK403 pKa = 11.6 LDD405 pKa = 4.16 AQMALPEE412 pKa = 4.58 KK413 pKa = 9.71 EE414 pKa = 4.61 DD415 pKa = 3.43 LSAYY419 pKa = 6.32 EE420 pKa = 3.96 TGVRR424 pKa = 11.84 LIGLVWAYY432 pKa = 9.83 GAHH435 pKa = 5.67 YY436 pKa = 10.11 DD437 pKa = 3.21 IYY439 pKa = 11.33 LRR441 pKa = 11.84 ILKK444 pKa = 10.23 VYY446 pKa = 10.63 LSLKK450 pKa = 9.77 LKK452 pKa = 10.71 PEE454 pKa = 3.85 FHH456 pKa = 6.04 VQQLLSYY463 pKa = 9.24 SEE465 pKa = 4.07 KK466 pKa = 9.94 PEE468 pKa = 3.68 RR469 pKa = 11.84 TKK471 pKa = 10.5 RR472 pKa = 11.84 YY473 pKa = 9.12 QEE475 pKa = 4.03 NFFSSMKK482 pKa = 10.19 YY483 pKa = 9.78 QLNLDD488 pKa = 3.74 LDD490 pKa = 4.15 IYY492 pKa = 11.44 DD493 pKa = 4.95 LLAFPKK499 pKa = 10.35 FWDD502 pKa = 3.24 VSNRR506 pKa = 11.84 YY507 pKa = 8.86 FGSKK511 pKa = 8.98 YY512 pKa = 9.7 EE513 pKa = 3.99 RR514 pKa = 11.84 LDD516 pKa = 3.6 FRR518 pKa = 11.84 SHH520 pKa = 7.25 KK521 pKa = 10.48 IYY523 pKa = 11.0 GG524 pKa = 3.49
Molecular weight: 61.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.771
IPC2_protein 8.814
IPC_protein 8.712
Toseland 9.282
ProMoST 9.151
Dawson 9.619
Bjellqvist 9.385
Wikipedia 9.823
Rodwell 9.809
Grimsley 9.706
Solomon 9.633
Lehninger 9.589
Nozaki 9.399
DTASelect 9.341
Thurlkill 9.443
EMBOSS 9.736
Sillero 9.56
Patrickios 4.609
IPC_peptide 9.633
IPC2_peptide 7.995
IPC2.peptide.svr19 7.792
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
843
319
524
421.5
49.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.915 ± 0.247
1.305 ± 0.139
5.694 ± 0.027
5.694 ± 0.139
5.457 ± 1.231
4.033 ± 0.809
2.491 ± 0.674
7.117 ± 0.049
7.117 ± 0.616
10.558 ± 0.113
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.898 ± 0.323
4.508 ± 0.269
4.745 ± 0.476
3.559 ± 0.225
6.762 ± 0.261
7.236 ± 0.319
5.694 ± 0.97
5.219 ± 0.889
1.779 ± 0.279
5.219 ± 0.441
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here