Dorea sp. CAG:105

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Dorea; environmental samples

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2158 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5DAJ6|R5DAJ6_9FIRM PKS_ER domain-containing protein OS=Dorea sp. CAG:105 OX=1262872 GN=BN457_00793 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 10.24FIYY5 pKa = 9.48PAIFRR10 pKa = 11.84QTEE13 pKa = 3.7EE14 pKa = 4.15GSYY17 pKa = 10.33KK18 pKa = 10.65GYY20 pKa = 10.6FPDD23 pKa = 5.36LEE25 pKa = 4.27YY26 pKa = 10.84CYY28 pKa = 11.27GEE30 pKa = 5.43GEE32 pKa = 4.39TLDD35 pKa = 4.86DD36 pKa = 6.19AIDD39 pKa = 4.34SINDD43 pKa = 3.53AGKK46 pKa = 10.32DD47 pKa = 3.28WLTLEE52 pKa = 4.42LSEE55 pKa = 5.55DD56 pKa = 3.94EE57 pKa = 4.33PLIPYY62 pKa = 10.14VSDD65 pKa = 5.18LEE67 pKa = 4.75DD68 pKa = 3.37LTLEE72 pKa = 4.26EE73 pKa = 4.67GDD75 pKa = 3.32IVRR78 pKa = 11.84NISMNIRR85 pKa = 11.84FYY87 pKa = 11.05EE88 pKa = 4.51GWDD91 pKa = 3.35EE92 pKa = 4.06

Molecular weight: 10.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5D955|R5D955_9FIRM Transcriptional repressor NrdR OS=Dorea sp. CAG:105 OX=1262872 GN=nrdR PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.79KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.2VHH16 pKa = 5.65GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2158

0

2158

684930

29

2228

317.4

35.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.526 ± 0.054

1.461 ± 0.019

5.611 ± 0.047

7.822 ± 0.071

3.821 ± 0.036

7.192 ± 0.046

1.833 ± 0.028

7.325 ± 0.047

7.875 ± 0.052

8.631 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.204 ± 0.032

4.126 ± 0.031

3.181 ± 0.029

3.103 ± 0.032

4.213 ± 0.039

5.396 ± 0.038

5.541 ± 0.042

7.156 ± 0.043

0.823 ± 0.015

4.161 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski