Amino acid dipepetide frequency for Bacillus sp. FJAT-21945

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.643AlaAla: 5.643 ± 0.085
0.591AlaCys: 0.591 ± 0.021
3.282AlaAsp: 3.282 ± 0.047
4.621AlaGlu: 4.621 ± 0.067
3.273AlaPhe: 3.273 ± 0.057
5.196AlaGly: 5.196 ± 0.068
1.187AlaHis: 1.187 ± 0.032
6.192AlaIle: 6.192 ± 0.08
4.62AlaLys: 4.62 ± 0.068
6.87AlaLeu: 6.87 ± 0.075
1.939AlaMet: 1.939 ± 0.039
2.835AlaAsn: 2.835 ± 0.049
1.905AlaPro: 1.905 ± 0.037
1.978AlaGln: 1.978 ± 0.041
2.456AlaArg: 2.456 ± 0.046
3.91AlaSer: 3.91 ± 0.064
3.068AlaThr: 3.068 ± 0.048
5.127AlaVal: 5.127 ± 0.076
0.574AlaTrp: 0.574 ± 0.023
2.221AlaTyr: 2.221 ± 0.037
0.0AlaXaa: 0.0 ± 0.0
Cys
0.451CysAla: 0.451 ± 0.018
0.098CysCys: 0.098 ± 0.009
0.382CysAsp: 0.382 ± 0.02
0.496CysGlu: 0.496 ± 0.022
0.352CysPhe: 0.352 ± 0.015
0.639CysGly: 0.639 ± 0.021
0.217CysHis: 0.217 ± 0.013
0.642CysIle: 0.642 ± 0.022
0.448CysLys: 0.448 ± 0.019
0.722CysLeu: 0.722 ± 0.022
0.196CysMet: 0.196 ± 0.012
0.285CysAsn: 0.285 ± 0.014
0.374CysPro: 0.374 ± 0.02
0.273CysGln: 0.273 ± 0.014
0.296CysArg: 0.296 ± 0.015
0.531CysSer: 0.531 ± 0.019
0.421CysThr: 0.421 ± 0.017
0.415CysVal: 0.415 ± 0.017
0.075CysTrp: 0.075 ± 0.007
0.261CysTyr: 0.261 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
3.046AspAla: 3.046 ± 0.047
0.387AspCys: 0.387 ± 0.017
2.237AspAsp: 2.237 ± 0.041
4.245AspGlu: 4.245 ± 0.068
2.395AspPhe: 2.395 ± 0.05
3.172AspGly: 3.172 ± 0.054
1.161AspHis: 1.161 ± 0.027
4.229AspIle: 4.229 ± 0.056
3.241AspLys: 3.241 ± 0.052
4.942AspLeu: 4.942 ± 0.062
1.296AspMet: 1.296 ± 0.032
1.788AspAsn: 1.788 ± 0.045
1.928AspPro: 1.928 ± 0.039
1.931AspGln: 1.931 ± 0.036
2.151AspArg: 2.151 ± 0.044
2.606AspSer: 2.606 ± 0.047
2.204AspThr: 2.204 ± 0.045
3.404AspVal: 3.404 ± 0.056
0.613AspTrp: 0.613 ± 0.024
2.086AspTyr: 2.086 ± 0.04
0.0AspXaa: 0.0 ± 0.0
Glu
5.082GluAla: 5.082 ± 0.072
0.398GluCys: 0.398 ± 0.017
3.671GluAsp: 3.671 ± 0.056
7.005GluGlu: 7.005 ± 0.089
2.732GluPhe: 2.732 ± 0.043
4.316GluGly: 4.316 ± 0.057
1.426GluHis: 1.426 ± 0.033
6.261GluIle: 6.261 ± 0.078
7.148GluLys: 7.148 ± 0.085
7.317GluLeu: 7.317 ± 0.085
2.558GluMet: 2.558 ± 0.046
3.936GluAsn: 3.936 ± 0.051
1.9GluPro: 1.9 ± 0.043
3.104GluGln: 3.104 ± 0.056
3.283GluArg: 3.283 ± 0.054
3.555GluSer: 3.555 ± 0.054
3.686GluThr: 3.686 ± 0.054
4.88GluVal: 4.88 ± 0.065
0.869GluTrp: 0.869 ± 0.027
2.453GluTyr: 2.453 ± 0.046
0.0GluXaa: 0.0 ± 0.0
Phe
3.083PheAla: 3.083 ± 0.051
0.382PheCys: 0.382 ± 0.018
2.356PheAsp: 2.356 ± 0.041
2.991PheGlu: 2.991 ± 0.049
2.548PhePhe: 2.548 ± 0.057
3.328PheGly: 3.328 ± 0.057
1.031PheHis: 1.031 ± 0.027
4.381PheIle: 4.381 ± 0.066
2.549PheLys: 2.549 ± 0.05
4.883PheLeu: 4.883 ± 0.068
1.219PheMet: 1.219 ± 0.028
2.157PheAsn: 2.157 ± 0.044
1.763PhePro: 1.763 ± 0.039
1.751PheGln: 1.751 ± 0.034
1.496PheArg: 1.496 ± 0.036
3.376PheSer: 3.376 ± 0.057
2.594PheThr: 2.594 ± 0.05
3.068PheVal: 3.068 ± 0.05
0.487PheTrp: 0.487 ± 0.022
1.812PheTyr: 1.812 ± 0.037
0.0PheXaa: 0.0 ± 0.0
Gly
4.573GlyAla: 4.573 ± 0.071
0.642GlyCys: 0.642 ± 0.02
3.048GlyAsp: 3.048 ± 0.056
4.53GlyGlu: 4.53 ± 0.065
3.512GlyPhe: 3.512 ± 0.056
4.595GlyGly: 4.595 ± 0.066
1.32GlyHis: 1.32 ± 0.035
6.189GlyIle: 6.189 ± 0.074
5.241GlyLys: 5.241 ± 0.055
6.635GlyLeu: 6.635 ± 0.083
2.18GlyMet: 2.18 ± 0.045
2.805GlyAsn: 2.805 ± 0.053
1.666GlyPro: 1.666 ± 0.035
2.152GlyGln: 2.152 ± 0.04
2.53GlyArg: 2.53 ± 0.044
3.924GlySer: 3.924 ± 0.057
3.862GlyThr: 3.862 ± 0.058
4.726GlyVal: 4.726 ± 0.058
0.815GlyTrp: 0.815 ± 0.03
2.796GlyTyr: 2.796 ± 0.052
0.0GlyXaa: 0.0 ± 0.0
His
1.26HisAla: 1.26 ± 0.031
0.225HisCys: 0.225 ± 0.011
0.91HisAsp: 0.91 ± 0.028
1.357HisGlu: 1.357 ± 0.032
1.161HisPhe: 1.161 ± 0.033
1.302HisGly: 1.302 ± 0.031
0.591HisHis: 0.591 ± 0.025
1.544HisIle: 1.544 ± 0.034
1.022HisLys: 1.022 ± 0.026
2.157HisLeu: 2.157 ± 0.042
0.529HisMet: 0.529 ± 0.019
0.781HisAsn: 0.781 ± 0.023
1.075HisPro: 1.075 ± 0.03
0.771HisGln: 0.771 ± 0.025
0.832HisArg: 0.832 ± 0.026
1.338HisSer: 1.338 ± 0.031
1.024HisThr: 1.024 ± 0.028
1.356HisVal: 1.356 ± 0.029
0.205HisTrp: 0.205 ± 0.013
0.871HisTyr: 0.871 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
6.13IleAla: 6.13 ± 0.081
0.741IleCys: 0.741 ± 0.025
4.454IleAsp: 4.454 ± 0.062
6.257IleGlu: 6.257 ± 0.075
3.736IlePhe: 3.736 ± 0.066
6.355IleGly: 6.355 ± 0.086
1.842IleHis: 1.842 ± 0.039
7.032IleIle: 7.032 ± 0.09
5.259IleLys: 5.259 ± 0.063
7.97IleLeu: 7.97 ± 0.1
1.973IleMet: 1.973 ± 0.042
3.867IleAsn: 3.867 ± 0.055
3.551IlePro: 3.551 ± 0.054
3.135IleGln: 3.135 ± 0.05
3.299IleArg: 3.299 ± 0.056
5.631IleSer: 5.631 ± 0.069
4.502IleThr: 4.502 ± 0.057
5.618IleVal: 5.618 ± 0.065
0.72IleTrp: 0.72 ± 0.023
2.668IleTyr: 2.668 ± 0.047
0.0IleXaa: 0.0 ± 0.0
Lys
4.669LysAla: 4.669 ± 0.072
0.399LysCys: 0.399 ± 0.018
4.054LysAsp: 4.054 ± 0.057
7.333LysGlu: 7.333 ± 0.087
2.093LysPhe: 2.093 ± 0.04
4.8LysGly: 4.8 ± 0.061
1.3LysHis: 1.3 ± 0.032
5.294LysIle: 5.294 ± 0.061
6.34LysLys: 6.34 ± 0.085
6.185LysLeu: 6.185 ± 0.073
2.371LysMet: 2.371 ± 0.04
3.729LysAsn: 3.729 ± 0.059
2.234LysPro: 2.234 ± 0.041
2.966LysGln: 2.966 ± 0.05
3.271LysArg: 3.271 ± 0.05
3.747LysSer: 3.747 ± 0.056
3.617LysThr: 3.617 ± 0.047
4.769LysVal: 4.769 ± 0.056
0.87LysTrp: 0.87 ± 0.026
2.338LysTyr: 2.338 ± 0.044
0.0LysXaa: 0.0 ± 0.0
Leu
7.03LeuAla: 7.03 ± 0.083
0.712LeuCys: 0.712 ± 0.022
4.673LeuAsp: 4.673 ± 0.064
6.793LeuGlu: 6.793 ± 0.078
5.099LeuPhe: 5.099 ± 0.082
6.381LeuGly: 6.381 ± 0.077
1.931LeuHis: 1.931 ± 0.045
8.012LeuIle: 8.012 ± 0.098
7.022LeuLys: 7.022 ± 0.074
10.441LeuLeu: 10.441 ± 0.115
2.502LeuMet: 2.502 ± 0.041
4.546LeuAsn: 4.546 ± 0.058
3.867LeuPro: 3.867 ± 0.049
3.395LeuGln: 3.395 ± 0.052
3.421LeuArg: 3.421 ± 0.049
7.054LeuSer: 7.054 ± 0.077
5.616LeuThr: 5.616 ± 0.07
6.061LeuVal: 6.061 ± 0.082
0.812LeuTrp: 0.812 ± 0.029
3.242LeuTyr: 3.242 ± 0.057
0.0LeuXaa: 0.0 ± 0.0
Met
2.0MetAla: 2.0 ± 0.041
0.148MetCys: 0.148 ± 0.01
1.53MetAsp: 1.53 ± 0.033
2.152MetGlu: 2.152 ± 0.047
1.063MetPhe: 1.063 ± 0.03
1.767MetGly: 1.767 ± 0.035
0.418MetHis: 0.418 ± 0.019
2.391MetIle: 2.391 ± 0.048
2.689MetLys: 2.689 ± 0.043
2.599MetLeu: 2.599 ± 0.046
0.845MetMet: 0.845 ± 0.027
1.725MetAsn: 1.725 ± 0.032
1.053MetPro: 1.053 ± 0.032
0.877MetGln: 0.877 ± 0.027
1.064MetArg: 1.064 ± 0.03
1.636MetSer: 1.636 ± 0.035
1.567MetThr: 1.567 ± 0.031
1.696MetVal: 1.696 ± 0.035
0.211MetTrp: 0.211 ± 0.012
0.794MetTyr: 0.794 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
2.651AsnAla: 2.651 ± 0.047
0.338AsnCys: 0.338 ± 0.015
2.317AsnAsp: 2.317 ± 0.037
4.009AsnGlu: 4.009 ± 0.061
1.76AsnPhe: 1.76 ± 0.036
3.505AsnGly: 3.505 ± 0.055
1.122AsnHis: 1.122 ± 0.031
3.816AsnIle: 3.816 ± 0.062
3.369AsnLys: 3.369 ± 0.057
4.009AsnLeu: 4.009 ± 0.055
1.28AsnMet: 1.28 ± 0.031
2.316AsnAsn: 2.316 ± 0.052
2.098AsnPro: 2.098 ± 0.041
1.956AsnGln: 1.956 ± 0.037
2.108AsnArg: 2.108 ± 0.039
2.49AsnSer: 2.49 ± 0.048
2.188AsnThr: 2.188 ± 0.048
2.914AsnVal: 2.914 ± 0.048
0.582AsnTrp: 0.582 ± 0.023
1.614AsnTyr: 1.614 ± 0.037
0.0AsnXaa: 0.0 ± 0.0
Pro
2.309ProAla: 2.309 ± 0.044
0.232ProCys: 0.232 ± 0.013
1.877ProAsp: 1.877 ± 0.037
2.832ProGlu: 2.832 ± 0.05
2.097ProPhe: 2.097 ± 0.042
2.171ProGly: 2.171 ± 0.048
0.736ProHis: 0.736 ± 0.025
3.066ProIle: 3.066 ± 0.052
2.203ProLys: 2.203 ± 0.038
3.455ProLeu: 3.455 ± 0.055
0.839ProMet: 0.839 ± 0.026
1.765ProAsn: 1.765 ± 0.036
1.04ProPro: 1.04 ± 0.029
1.082ProGln: 1.082 ± 0.028
1.076ProArg: 1.076 ± 0.031
2.312ProSer: 2.312 ± 0.041
1.835ProThr: 1.835 ± 0.038
2.611ProVal: 2.611 ± 0.047
0.363ProTrp: 0.363 ± 0.016
1.383ProTyr: 1.383 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
2.516GlnAla: 2.516 ± 0.043
0.222GlnCys: 0.222 ± 0.014
1.516GlnAsp: 1.516 ± 0.035
2.701GlnGlu: 2.701 ± 0.043
1.647GlnPhe: 1.647 ± 0.032
2.072GlnGly: 2.072 ± 0.045
0.693GlnHis: 0.693 ± 0.025
2.704GlnIle: 2.704 ± 0.046
2.734GlnLys: 2.734 ± 0.045
3.937GlnLeu: 3.937 ± 0.054
1.199GlnMet: 1.199 ± 0.029
1.643GlnAsn: 1.643 ± 0.04
1.127GlnPro: 1.127 ± 0.03
1.526GlnGln: 1.526 ± 0.048
1.342GlnArg: 1.342 ± 0.032
2.157GlnSer: 2.157 ± 0.041
1.972GlnThr: 1.972 ± 0.036
2.224GlnVal: 2.224 ± 0.045
0.363GlnTrp: 0.363 ± 0.016
1.32GlnTyr: 1.32 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
2.279ArgAla: 2.279 ± 0.043
0.262ArgCys: 0.262 ± 0.013
1.834ArgAsp: 1.834 ± 0.038
3.078ArgGlu: 3.078 ± 0.047
1.87ArgPhe: 1.87 ± 0.04
2.279ArgGly: 2.279 ± 0.048
0.731ArgHis: 0.731 ± 0.022
3.193ArgIle: 3.193 ± 0.051
3.302ArgLys: 3.302 ± 0.057
3.925ArgLeu: 3.925 ± 0.063
1.303ArgMet: 1.303 ± 0.03
1.94ArgAsn: 1.94 ± 0.039
1.23ArgPro: 1.23 ± 0.034
1.354ArgGln: 1.354 ± 0.032
1.73ArgArg: 1.73 ± 0.04
2.129ArgSer: 2.129 ± 0.039
1.956ArgThr: 1.956 ± 0.036
2.499ArgVal: 2.499 ± 0.05
0.39ArgTrp: 0.39 ± 0.017
1.419ArgTyr: 1.419 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
3.774SerAla: 3.774 ± 0.055
0.457SerCys: 0.457 ± 0.017
2.683SerAsp: 2.683 ± 0.05
4.022SerGlu: 4.022 ± 0.052
3.543SerPhe: 3.543 ± 0.054
4.399SerGly: 4.399 ± 0.059
1.263SerHis: 1.263 ± 0.027
5.573SerIle: 5.573 ± 0.079
4.083SerLys: 4.083 ± 0.053
6.358SerLeu: 6.358 ± 0.071
1.747SerMet: 1.747 ± 0.036
2.72SerAsn: 2.72 ± 0.048
2.213SerPro: 2.213 ± 0.045
1.927SerGln: 1.927 ± 0.04
2.199SerArg: 2.199 ± 0.042
4.087SerSer: 4.087 ± 0.073
3.002SerThr: 3.002 ± 0.05
3.98SerVal: 3.98 ± 0.051
0.644SerTrp: 0.644 ± 0.023
2.332SerTyr: 2.332 ± 0.044
0.0SerXaa: 0.0 ± 0.0
Thr
3.833ThrAla: 3.833 ± 0.052
0.344ThrCys: 0.344 ± 0.016
2.505ThrAsp: 2.505 ± 0.044
3.434ThrGlu: 3.434 ± 0.043
2.682ThrPhe: 2.682 ± 0.05
4.041ThrGly: 4.041 ± 0.055
0.996ThrHis: 0.996 ± 0.026
4.714ThrIle: 4.714 ± 0.059
3.275ThrLys: 3.275 ± 0.049
4.929ThrLeu: 4.929 ± 0.059
1.259ThrMet: 1.259 ± 0.033
2.458ThrAsn: 2.458 ± 0.042
2.053ThrPro: 2.053 ± 0.038
1.331ThrGln: 1.331 ± 0.036
1.771ThrArg: 1.771 ± 0.035
3.16ThrSer: 3.16 ± 0.044
2.649ThrThr: 2.649 ± 0.053
3.969ThrVal: 3.969 ± 0.068
0.493ThrTrp: 0.493 ± 0.018
1.815ThrTyr: 1.815 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
4.501ValAla: 4.501 ± 0.063
0.589ValCys: 0.589 ± 0.02
3.296ValAsp: 3.296 ± 0.052
4.432ValGlu: 4.432 ± 0.062
3.272ValPhe: 3.272 ± 0.054
4.28ValGly: 4.28 ± 0.06
1.289ValHis: 1.289 ± 0.031
5.912ValIle: 5.912 ± 0.078
4.759ValLys: 4.759 ± 0.058
6.524ValLeu: 6.524 ± 0.075
1.787ValMet: 1.787 ± 0.035
3.166ValAsn: 3.166 ± 0.047
2.53ValPro: 2.53 ± 0.043
2.298ValGln: 2.298 ± 0.042
2.414ValArg: 2.414 ± 0.045
4.503ValSer: 4.503 ± 0.064
3.684ValThr: 3.684 ± 0.054
4.501ValVal: 4.501 ± 0.061
0.615ValTrp: 0.615 ± 0.019
2.2ValTyr: 2.2 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
0.617TrpAla: 0.617 ± 0.025
0.077TrpCys: 0.077 ± 0.008
0.54TrpAsp: 0.54 ± 0.021
0.692TrpGlu: 0.692 ± 0.026
0.495TrpPhe: 0.495 ± 0.02
0.7TrpGly: 0.7 ± 0.022
0.214TrpHis: 0.214 ± 0.013
0.905TrpIle: 0.905 ± 0.032
0.794TrpLys: 0.794 ± 0.024
1.127TrpLeu: 1.127 ± 0.032
0.337TrpMet: 0.337 ± 0.016
0.517TrpAsn: 0.517 ± 0.021
0.258TrpPro: 0.258 ± 0.015
0.338TrpGln: 0.338 ± 0.017
0.407TrpArg: 0.407 ± 0.018
0.594TrpSer: 0.594 ± 0.022
0.529TrpThr: 0.529 ± 0.021
0.633TrpVal: 0.633 ± 0.024
0.154TrpTrp: 0.154 ± 0.011
0.34TrpTyr: 0.34 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.04TyrAla: 2.04 ± 0.038
0.333TyrCys: 0.333 ± 0.015
1.895TyrAsp: 1.895 ± 0.046
2.598TyrGlu: 2.598 ± 0.042
1.928TyrPhe: 1.928 ± 0.04
2.441TyrGly: 2.441 ± 0.046
0.843TyrHis: 0.843 ± 0.025
2.69TyrIle: 2.69 ± 0.05
2.283TyrLys: 2.283 ± 0.044
3.626TyrLeu: 3.626 ± 0.053
0.864TyrMet: 0.864 ± 0.026
1.462TyrAsn: 1.462 ± 0.036
1.422TyrPro: 1.422 ± 0.033
1.469TyrGln: 1.469 ± 0.036
1.556TyrArg: 1.556 ± 0.035
2.266TyrSer: 2.266 ± 0.042
1.735TyrThr: 1.735 ± 0.037
2.104TyrVal: 2.104 ± 0.037
0.412TyrTrp: 0.412 ± 0.018
1.495TyrTyr: 1.495 ± 0.039
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4788 proteins (1362251 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski