Pacmanvirus A23

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 465 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X6WEV1|A0A1X6WEV1_9VIRU Thioredoxin OS=Pacmanvirus A23 OX=1932881 GN=PACV_123 PE=4 SV=1
MM1 pKa = 7.71EE2 pKa = 5.86SYY4 pKa = 10.65LIAIIIVVTIFILYY18 pKa = 10.37SIATWNVNAYY28 pKa = 9.4EE29 pKa = 4.09DD30 pKa = 4.16YY31 pKa = 10.71IYY33 pKa = 10.75GFWVAEE39 pKa = 3.96DD40 pKa = 5.29DD41 pKa = 4.34EE42 pKa = 4.68FCKK45 pKa = 10.82DD46 pKa = 3.63SEE48 pKa = 4.3IDD50 pKa = 3.97SMLLFIGKK58 pKa = 8.8PEE60 pKa = 4.56DD61 pKa = 3.49SWCSRR66 pKa = 11.84QRR68 pKa = 11.84TCYY71 pKa = 10.31LIIMNNICAQGLKK84 pKa = 9.42IDD86 pKa = 4.7YY87 pKa = 7.59KK88 pKa = 10.49TGWAGIGIGKK98 pKa = 9.59YY99 pKa = 9.82KK100 pKa = 10.19IKK102 pKa = 10.82CNAEE106 pKa = 3.77FDD108 pKa = 5.12DD109 pKa = 4.11EE110 pKa = 4.71QIWDD114 pKa = 3.88EE115 pKa = 4.28NIEE118 pKa = 4.41IIVDD122 pKa = 3.68MLTGAMKK129 pKa = 10.44IMSCDD134 pKa = 2.92GTVYY138 pKa = 11.08AKK140 pKa = 10.61LNKK143 pKa = 9.48QHH145 pKa = 7.03DD146 pKa = 4.26VSNLADD152 pKa = 3.5QMEE155 pKa = 4.67DD156 pKa = 3.07ADD158 pKa = 5.01MIDD161 pKa = 3.51EE162 pKa = 4.3

Molecular weight: 18.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X6WGK1|A0A1X6WGK1_9VIRU Uncharacterized protein OS=Pacmanvirus A23 OX=1932881 GN=PACV_182 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.12NAQIILNPFTGGKK15 pKa = 9.26GATVVIIYY23 pKa = 10.21GGTASGSQATGGRR36 pKa = 3.76

Molecular weight: 3.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

465

0

465

117397

36

3391

252.5

28.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.273 ± 0.137

2.096 ± 0.094

6.05 ± 0.09

6.802 ± 0.163

4.261 ± 0.076

4.831 ± 0.173

1.921 ± 0.053

8.658 ± 0.115

8.456 ± 0.158

7.903 ± 0.106

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.457 ± 0.048

6.743 ± 0.093

3.456 ± 0.06

3.288 ± 0.071

4.225 ± 0.076

6.349 ± 0.117

5.791 ± 0.142

5.63 ± 0.096

1.003 ± 0.039

4.808 ± 0.088
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski