Hubei sobemo-like virus 42

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEL0|A0A1L3KEL0_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 42 OX=1923230 PE=4 SV=1
MM1 pKa = 7.96DD2 pKa = 5.97RR3 pKa = 11.84ILYY6 pKa = 10.39VMEE9 pKa = 3.79QLYY12 pKa = 10.34EE13 pKa = 4.21SVRR16 pKa = 11.84VDD18 pKa = 3.21HH19 pKa = 6.69YY20 pKa = 12.09NWFCYY25 pKa = 10.74DD26 pKa = 3.15NFLKK30 pKa = 10.69VVRR33 pKa = 11.84CLEE36 pKa = 4.34FSSSPGYY43 pKa = 10.49GFSRR47 pKa = 11.84TAPTIGGWLKK57 pKa = 10.56FDD59 pKa = 4.53GMNFDD64 pKa = 3.94PQRR67 pKa = 11.84LSEE70 pKa = 4.3LWHH73 pKa = 5.87MVNVCYY79 pKa = 10.4RR80 pKa = 11.84EE81 pKa = 4.18PKK83 pKa = 10.15LFSYY87 pKa = 9.59WKK89 pKa = 10.08VFIKK93 pKa = 10.57RR94 pKa = 11.84EE95 pKa = 3.84PHH97 pKa = 5.54KK98 pKa = 9.61TSKK101 pKa = 10.09MEE103 pKa = 3.96NKK105 pKa = 9.24RR106 pKa = 11.84WRR108 pKa = 11.84LIQCCPLDD116 pKa = 4.22VQVLWHH122 pKa = 5.95MLFAKK127 pKa = 10.59SNEE130 pKa = 3.99LEE132 pKa = 4.4ANEE135 pKa = 4.37SMSIPSVQGMVLPFGGWKK153 pKa = 8.62EE154 pKa = 4.14HH155 pKa = 5.37YY156 pKa = 9.68NRR158 pKa = 11.84WMKK161 pKa = 10.99NKK163 pKa = 10.11LFFGSDD169 pKa = 2.56KK170 pKa = 10.88TAWDD174 pKa = 3.45WTASEE179 pKa = 4.16WMIEE183 pKa = 3.77LDD185 pKa = 3.36LEE187 pKa = 4.08LRR189 pKa = 11.84RR190 pKa = 11.84RR191 pKa = 11.84LIHH194 pKa = 7.15TDD196 pKa = 3.69DD197 pKa = 3.86DD198 pKa = 4.95WITQAAKK205 pKa = 9.94VYY207 pKa = 10.67EE208 pKa = 4.07NAFYY212 pKa = 10.48DD213 pKa = 3.33AHH215 pKa = 7.15LILGDD220 pKa = 3.41GRR222 pKa = 11.84IYY224 pKa = 10.44RR225 pKa = 11.84QMYY228 pKa = 8.91PGIIKK233 pKa = 10.05SGCVNTISINSRR245 pKa = 11.84CQVMLHH251 pKa = 6.52LLYY254 pKa = 10.7SFRR257 pKa = 11.84KK258 pKa = 9.7GISPYY263 pKa = 10.33PMVVAVGDD271 pKa = 3.76DD272 pKa = 3.43TLQAEE277 pKa = 4.41EE278 pKa = 4.44HH279 pKa = 6.64AVDD282 pKa = 3.12KK283 pKa = 11.21QMYY286 pKa = 8.45EE287 pKa = 3.81RR288 pKa = 11.84FGVVIKK294 pKa = 10.76SVSEE298 pKa = 3.77TLEE301 pKa = 3.72FLGRR305 pKa = 11.84EE306 pKa = 4.25WNDD309 pKa = 3.27DD310 pKa = 3.79GPRR313 pKa = 11.84PMYY316 pKa = 9.88VSKK319 pKa = 10.7HH320 pKa = 5.19IFSLCYY326 pKa = 10.28KK327 pKa = 10.11NIEE330 pKa = 4.27LTPQILDD337 pKa = 3.23ALMRR341 pKa = 11.84EE342 pKa = 4.49YY343 pKa = 11.42VNEE346 pKa = 3.78DD347 pKa = 3.19SMFNFLTILTRR358 pKa = 11.84EE359 pKa = 4.36LGFSSNVHH367 pKa = 5.3SRR369 pKa = 11.84DD370 pKa = 3.5YY371 pKa = 10.79YY372 pKa = 10.75KK373 pKa = 11.14YY374 pKa = 10.57WMDD377 pKa = 4.56NPDD380 pKa = 3.48AQYY383 pKa = 11.69LHH385 pKa = 7.0

Molecular weight: 45.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEL0|A0A1L3KEL0_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 42 OX=1923230 PE=4 SV=1
MM1 pKa = 7.9EE2 pKa = 5.62DD3 pKa = 3.29QTVLALIKK11 pKa = 10.52QLVRR15 pKa = 11.84TMGSNAWNLFKK26 pKa = 11.13DD27 pKa = 4.52FLTSSYY33 pKa = 10.16VRR35 pKa = 11.84NFAALAVSSMALYY48 pKa = 10.73SRR50 pKa = 11.84INSWLRR56 pKa = 11.84EE57 pKa = 4.08NNTSILEE64 pKa = 4.06YY65 pKa = 10.73LLKK68 pKa = 10.0TSKK71 pKa = 10.82LFLSVTKK78 pKa = 10.3ILSWFRR84 pKa = 11.84KK85 pKa = 9.09PKK87 pKa = 10.23DD88 pKa = 3.42PRR90 pKa = 11.84PMVRR94 pKa = 11.84TSWAAQRR101 pKa = 11.84FPEE104 pKa = 4.67SIVQGSEE111 pKa = 3.71EE112 pKa = 4.01TEE114 pKa = 4.02MFHH117 pKa = 7.48KK118 pKa = 10.42RR119 pKa = 11.84GEE121 pKa = 4.12ALVVDD126 pKa = 4.29VNGNAVGKK134 pKa = 9.39GARR137 pKa = 11.84FWNVLVVPEE146 pKa = 4.54HH147 pKa = 6.65VIGTCRR153 pKa = 11.84YY154 pKa = 8.06SSGSSVVYY162 pKa = 9.66ICHH165 pKa = 7.63PDD167 pKa = 3.34RR168 pKa = 11.84PDD170 pKa = 3.91CKK172 pKa = 10.62FEE174 pKa = 4.11LAGEE178 pKa = 4.17PVFLYY183 pKa = 10.88SDD185 pKa = 3.9LLGYY189 pKa = 10.0NVSSSTWSQLGISQVSIGWITKK211 pKa = 7.29PTAASVVGVKK221 pKa = 10.37RR222 pKa = 11.84FGTSGILKK230 pKa = 10.11VDD232 pKa = 3.82DD233 pKa = 4.27EE234 pKa = 5.21SFGSVRR240 pKa = 11.84YY241 pKa = 7.99MATTCHH247 pKa = 6.67GYY249 pKa = 10.3SGALYY254 pKa = 9.95TSGNLTLGIHH264 pKa = 6.64LNGGKK269 pKa = 10.1KK270 pKa = 10.1NLGIAADD277 pKa = 4.17FVRR280 pKa = 11.84ALVSSKK286 pKa = 9.18MDD288 pKa = 3.11KK289 pKa = 10.76TMEE292 pKa = 4.45SSDD295 pKa = 3.71DD296 pKa = 3.83DD297 pKa = 3.4TWDD300 pKa = 4.89FINQRR305 pKa = 11.84WFDD308 pKa = 3.63GGKK311 pKa = 9.94LRR313 pKa = 11.84DD314 pKa = 3.96GVKK317 pKa = 10.09VDD319 pKa = 4.48YY320 pKa = 9.52ITADD324 pKa = 3.64EE325 pKa = 5.24IYY327 pKa = 10.38ASSQGRR333 pKa = 11.84FHH335 pKa = 7.14VVNKK339 pKa = 10.56DD340 pKa = 3.28VFRR343 pKa = 11.84KK344 pKa = 9.33HH345 pKa = 6.72ASEE348 pKa = 4.24EE349 pKa = 3.98QWNSLVYY356 pKa = 10.48ADD358 pKa = 5.17RR359 pKa = 11.84EE360 pKa = 4.43VEE362 pKa = 4.57SVFPLGAPRR371 pKa = 11.84LSPGASNMSEE381 pKa = 4.05KK382 pKa = 9.52VTAPTRR388 pKa = 11.84KK389 pKa = 9.68VISGGTAEE397 pKa = 5.2LQQQLQRR404 pKa = 11.84FAASPSGSRR413 pKa = 11.84SKK415 pKa = 10.93GAEE418 pKa = 3.66KK419 pKa = 10.16RR420 pKa = 11.84YY421 pKa = 9.46GWIRR425 pKa = 11.84ALTEE429 pKa = 4.21TEE431 pKa = 4.78LMDD434 pKa = 3.11YY435 pKa = 10.09WRR437 pKa = 11.84LLKK440 pKa = 10.28EE441 pKa = 4.19DD442 pKa = 4.33QEE444 pKa = 4.57NASQQ448 pKa = 3.65

Molecular weight: 49.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

833

385

448

416.5

47.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.522 ± 1.263

1.441 ± 0.427

5.642 ± 0.396

6.002 ± 0.329

4.682 ± 0.343

6.363 ± 0.956

2.281 ± 0.56

4.562 ± 0.424

5.642 ± 0.126

8.764 ± 0.219

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.361 ± 0.88

4.322 ± 0.237

3.481 ± 0.278

3.241 ± 0.083

6.002 ± 0.019

9.124 ± 1.413

4.562 ± 0.794

7.443 ± 0.636

3.121 ± 0.345

4.442 ± 0.852
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski