Alphaproteobacteria bacterium PA2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; unclassified Alphaproteobacteria

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3257 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A257J804|A0A257J804_9PROT Ni_hydr_CYTB domain-containing protein OS=Alphaproteobacteria bacterium PA2 OX=2015570 GN=CFE28_15275 PE=4 SV=1
MM1 pKa = 7.3SWDD4 pKa = 3.37SANAYY9 pKa = 9.85ASSLGGHH16 pKa = 6.9LAFITSAAEE25 pKa = 3.59NDD27 pKa = 3.95YY28 pKa = 10.73LHH30 pKa = 7.21AMAQSDD36 pKa = 4.3VQLLATAPTASDD48 pKa = 3.33GGGARR53 pKa = 11.84YY54 pKa = 8.79LWLGGSDD61 pKa = 3.48AAVEE65 pKa = 4.52GAWRR69 pKa = 11.84WSDD72 pKa = 3.13GSLLSGYY79 pKa = 6.78TNWGAGSLGVEE90 pKa = 3.88PDD92 pKa = 3.46NFFGLQDD99 pKa = 3.52GMALGLQSWPQPSGGIGIAGKK120 pKa = 9.7WNDD123 pKa = 4.4LNPADD128 pKa = 4.64LLYY131 pKa = 10.55FYY133 pKa = 11.22VEE135 pKa = 4.08YY136 pKa = 10.89DD137 pKa = 3.85SIQGTSGNDD146 pKa = 3.17SLIYY150 pKa = 10.01SSSSITSFGGAISIFGGTGNDD171 pKa = 3.49TIEE174 pKa = 4.41AQGGTNYY181 pKa = 10.25LRR183 pKa = 11.84GEE185 pKa = 4.75DD186 pKa = 4.31GNDD189 pKa = 3.77CIIGGTGFDD198 pKa = 5.2DD199 pKa = 3.15IHH201 pKa = 7.16GNKK204 pKa = 9.93GDD206 pKa = 4.69DD207 pKa = 4.01IIIGGPGGDD216 pKa = 3.41WLVGGQGNDD225 pKa = 4.14LITSTGGVDD234 pKa = 3.01ILYY237 pKa = 10.91GNLGNDD243 pKa = 3.97TLNGGPDD250 pKa = 3.76ADD252 pKa = 4.6LMRR255 pKa = 11.84GGQADD260 pKa = 3.91DD261 pKa = 4.37LLNGGGGNDD270 pKa = 3.44WLSGDD275 pKa = 4.32RR276 pKa = 11.84GSDD279 pKa = 3.51TITGGTGADD288 pKa = 3.06IFHH291 pKa = 6.96TFGDD295 pKa = 3.57AGIDD299 pKa = 3.14RR300 pKa = 11.84VTDD303 pKa = 4.08FNLSEE308 pKa = 4.23GDD310 pKa = 3.85RR311 pKa = 11.84VQLDD315 pKa = 3.53PGTVYY320 pKa = 10.56TVTQVGADD328 pKa = 3.41TVINMTGGGQMTLVGVQMATLPAGWIFGAA357 pKa = 4.54

Molecular weight: 36.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A257J3X0|A0A257J3X0_9PROT N-acetylmuramoyl-L-alanine amidase OS=Alphaproteobacteria bacterium PA2 OX=2015570 GN=CFE28_06990 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 3.78GWRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.45NGQKK29 pKa = 10.22VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.88GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3257

0

3257

1055213

29

1685

324.0

34.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.998 ± 0.052

0.772 ± 0.012

5.823 ± 0.033

5.357 ± 0.039

3.672 ± 0.025

9.003 ± 0.05

1.865 ± 0.019

4.711 ± 0.027

3.376 ± 0.029

10.003 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.474 ± 0.019

2.559 ± 0.026

5.456 ± 0.035

3.268 ± 0.025

6.857 ± 0.043

5.503 ± 0.032

5.34 ± 0.039

7.341 ± 0.03

1.414 ± 0.019

2.207 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski