Sulfolobus spindle-shaped virus

Taxonomy: Viruses; Fuselloviridae; Alphafusellovirus; unclassified Alphafusellovirus

Average proteome isoelectric point is 7.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8G8D3|A0A3G8G8D3_9VIRU Uncharacterized protein OS=Sulfolobus spindle-shaped virus OX=2491899 PE=4 SV=1
MM1 pKa = 7.46SALGDD6 pKa = 3.62VIYY9 pKa = 10.69VVSILFPAVGLISRR23 pKa = 11.84NYY25 pKa = 9.87LVNLMGTFLGVIGFLVFVQGYY46 pKa = 7.1TDD48 pKa = 3.19IAFSGSTFYY57 pKa = 11.2LAIFPLLLGLVNLGFFFNWVRR78 pKa = 11.84EE79 pKa = 3.99EE80 pKa = 4.28RR81 pKa = 11.84II82 pKa = 3.48

Molecular weight: 9.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8G982|A0A3G8G982_9VIRU Uncharacterized protein OS=Sulfolobus spindle-shaped virus OX=2491899 PE=4 SV=1
MM1 pKa = 7.87RR2 pKa = 11.84WGSDD6 pKa = 3.03DD7 pKa = 3.93TYY9 pKa = 10.35TGKK12 pKa = 9.61IRR14 pKa = 11.84RR15 pKa = 11.84NSPRR19 pKa = 11.84FRR21 pKa = 11.84FNYY24 pKa = 8.03IDD26 pKa = 3.78YY27 pKa = 11.0VITIQKK33 pKa = 10.53AKK35 pKa = 10.33AQEE38 pKa = 3.96KK39 pKa = 8.55TIKK42 pKa = 9.73RR43 pKa = 11.84TIDD46 pKa = 2.63EE47 pKa = 4.48WKK49 pKa = 8.53TSRR52 pKa = 11.84VLSRR56 pKa = 11.84RR57 pKa = 11.84GFGISLTRR65 pKa = 11.84RR66 pKa = 11.84ALCLLHH72 pKa = 6.68HH73 pKa = 6.86LRR75 pKa = 11.84NLFYY79 pKa = 11.04GIIDD83 pKa = 3.46

Molecular weight: 9.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26

0

26

4183

50

793

160.9

18.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.331 ± 0.268

0.526 ± 0.183

2.606 ± 0.446

4.662 ± 0.902

5.331 ± 0.403

6.933 ± 0.611

1.052 ± 0.3

8.774 ± 0.507

6.216 ± 1.098

10.853 ± 0.856

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.152 ± 0.349

5.57 ± 0.803

4.566 ± 0.437

3.108 ± 0.37

3.466 ± 0.665

8.104 ± 0.842

7.076 ± 0.61

6.718 ± 0.422

1.387 ± 0.207

5.57 ± 0.834
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski