Lupine feces-associated gemycircularvirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus canlup1

Average proteome isoelectric point is 7.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A221LEB9|A0A221LEB9_9VIRU Capsid OS=Lupine feces-associated gemycircularvirus 2 OX=2017710 PE=4 SV=1
MM1 pKa = 7.34TFILNARR8 pKa = 11.84YY9 pKa = 8.98FLVTYY14 pKa = 6.9PQCGALDD21 pKa = 3.46EE22 pKa = 4.54WTVNDD27 pKa = 3.94HH28 pKa = 6.5FGSLGAEE35 pKa = 4.14CIVGRR40 pKa = 11.84EE41 pKa = 3.9VHH43 pKa = 6.8ADD45 pKa = 3.25GGTHH49 pKa = 6.52LHH51 pKa = 5.73VFCDD55 pKa = 5.2FSRR58 pKa = 11.84KK59 pKa = 8.64FRR61 pKa = 11.84SRR63 pKa = 11.84RR64 pKa = 11.84ADD66 pKa = 2.97IFDD69 pKa = 3.92VQGHH73 pKa = 6.28HH74 pKa = 7.01PNIARR79 pKa = 11.84SKK81 pKa = 8.71RR82 pKa = 11.84NPRR85 pKa = 11.84KK86 pKa = 9.76GADD89 pKa = 3.4YY90 pKa = 10.44AAKK93 pKa = 10.7DD94 pKa = 3.57GDD96 pKa = 4.02IVAGGLDD103 pKa = 3.42IGGLPTSLVSGAQDD117 pKa = 3.4PGSTLVACEE126 pKa = 3.6NRR128 pKa = 11.84AEE130 pKa = 4.06FFEE133 pKa = 4.61VAEE136 pKa = 5.42DD137 pKa = 3.53MCPWDD142 pKa = 5.53LITKK146 pKa = 8.91FGSMYY151 pKa = 10.73AYY153 pKa = 10.22AKK155 pKa = 9.31WKK157 pKa = 8.92WPEE160 pKa = 3.13IGMGYY165 pKa = 9.55EE166 pKa = 3.98RR167 pKa = 11.84PPGLVLTDD175 pKa = 3.55GAFPDD180 pKa = 3.67LVSYY184 pKa = 11.01RR185 pKa = 11.84EE186 pKa = 4.44AILDD190 pKa = 3.36VRR192 pKa = 11.84GKK194 pKa = 10.68VKK196 pKa = 9.79TLVIHH201 pKa = 6.63GPSLTGKK208 pKa = 6.01TTWAQSLGNHH218 pKa = 6.02IYY220 pKa = 9.7MRR222 pKa = 11.84ARR224 pKa = 11.84YY225 pKa = 7.94NARR228 pKa = 11.84EE229 pKa = 3.91ASLCEE234 pKa = 3.99GKK236 pKa = 10.24DD237 pKa = 3.19YY238 pKa = 11.45CVIDD242 pKa = 5.29DD243 pKa = 3.61ISGGIRR249 pKa = 11.84FFPHH253 pKa = 5.06WKK255 pKa = 9.76DD256 pKa = 2.9WFGGQPYY263 pKa = 9.56VQIRR267 pKa = 11.84LLYY270 pKa = 9.63RR271 pKa = 11.84DD272 pKa = 3.79EE273 pKa = 4.46VLLRR277 pKa = 11.84WGKK280 pKa = 7.44PTIWLNNRR288 pKa = 11.84DD289 pKa = 3.78PRR291 pKa = 11.84DD292 pKa = 3.39QLRR295 pKa = 11.84DD296 pKa = 3.57MVGRR300 pKa = 11.84DD301 pKa = 3.63YY302 pKa = 11.45TDD304 pKa = 3.12EE305 pKa = 4.07QYY307 pKa = 11.42HH308 pKa = 6.69NDD310 pKa = 4.06VEE312 pKa = 4.54WMDD315 pKa = 3.87ANCIFVYY322 pKa = 10.21VDD324 pKa = 3.3SPIVTFHH331 pKa = 7.16ANTEE335 pKa = 4.1

Molecular weight: 38.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A221LEB9|A0A221LEB9_9VIRU Capsid OS=Lupine feces-associated gemycircularvirus 2 OX=2017710 PE=4 SV=1
MM1 pKa = 7.54ASSGRR6 pKa = 11.84RR7 pKa = 11.84TRR9 pKa = 11.84RR10 pKa = 11.84SAPRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84PGSRR21 pKa = 11.84RR22 pKa = 11.84FGGTRR27 pKa = 11.84SRR29 pKa = 11.84KK30 pKa = 9.23SRR32 pKa = 11.84ITRR35 pKa = 11.84RR36 pKa = 11.84TYY38 pKa = 10.25RR39 pKa = 11.84KK40 pKa = 9.42NRR42 pKa = 11.84AMPKK46 pKa = 9.96KK47 pKa = 9.51RR48 pKa = 11.84VLNITSKK55 pKa = 10.61KK56 pKa = 9.1KK57 pKa = 10.22RR58 pKa = 11.84NGMLSWSNTNPSGGSQTTALGPAYY82 pKa = 10.69VNASTGGLFLFCPTAQNLNTAGGANLAIDD111 pKa = 3.8VSEE114 pKa = 4.39RR115 pKa = 11.84TASTCYY121 pKa = 9.12MKK123 pKa = 10.84GFSEE127 pKa = 5.11HH128 pKa = 6.56IRR130 pKa = 11.84IQTSSPIPWFWRR142 pKa = 11.84RR143 pKa = 11.84ICFTVKK149 pKa = 10.36GYY151 pKa = 9.64PFSSSTSPTNSEE163 pKa = 3.49ATYY166 pKa = 11.2LDD168 pKa = 3.52TTNGLEE174 pKa = 4.36RR175 pKa = 11.84LWLNIGINNSTTFYY189 pKa = 11.43NSITSLLFKK198 pKa = 10.7GVSQKK203 pKa = 11.23DD204 pKa = 3.15WTDD207 pKa = 3.37PLIAPLDD214 pKa = 3.81TSRR217 pKa = 11.84VTVKK221 pKa = 10.3FDD223 pKa = 3.3KK224 pKa = 10.46TWTIKK229 pKa = 10.58SGNAAGAVTEE239 pKa = 4.29RR240 pKa = 11.84KK241 pKa = 9.69HH242 pKa = 5.13YY243 pKa = 10.82FPMNHH248 pKa = 6.08NLVYY252 pKa = 10.92DD253 pKa = 4.21DD254 pKa = 5.36DD255 pKa = 4.9EE256 pKa = 6.3NGAAMTTSYY265 pKa = 11.49YY266 pKa = 11.02SVDD269 pKa = 3.49SKK271 pKa = 11.83AGMGDD276 pKa = 4.03YY277 pKa = 10.86FIMDD281 pKa = 4.08YY282 pKa = 10.83FSAGTGGGIGDD293 pKa = 5.75LIRR296 pKa = 11.84IDD298 pKa = 4.52SNATLYY304 pKa = 9.33WHH306 pKa = 7.03EE307 pKa = 4.27KK308 pKa = 9.3

Molecular weight: 34.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

643

308

335

321.5

36.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.998 ± 0.121

1.711 ± 0.495

6.687 ± 1.439

3.577 ± 0.876

4.51 ± 0.194

9.02 ± 0.171

2.333 ± 0.695

5.288 ± 0.062

4.666 ± 0.574

6.532 ± 0.462

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.333 ± 0.178

4.977 ± 1.019

4.51 ± 0.194

1.866 ± 0.381

7.932 ± 0.561

7.309 ± 2.287

7.621 ± 2.078

5.132 ± 1.267

2.644 ± 0.249

4.355 ± 0.09
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski