Pantoea phage vB_PagS_AAS21

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; unclassified Demerecviridae

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 213 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5P1L3|A0A4Y5P1L3_9CAUD Uncharacterized protein OS=Pantoea phage vB_PagS_AAS21 OX=2575261 GN=AAS21_gp110 PE=4 SV=1
MM1 pKa = 7.55CNKK4 pKa = 9.95SVEE7 pKa = 4.15KK8 pKa = 10.6LALEE12 pKa = 4.27FVNGYY17 pKa = 9.3IAGSCVSYY25 pKa = 11.38DD26 pKa = 3.72DD27 pKa = 5.16ALTTFAEE34 pKa = 4.27ALTDD38 pKa = 3.65EE39 pKa = 5.88GITVPNSDD47 pKa = 4.74TLEE50 pKa = 3.82KK51 pKa = 9.79WLNDD55 pKa = 3.97AIEE58 pKa = 3.98EE59 pKa = 4.18SGIFNCDD66 pKa = 3.01EE67 pKa = 4.22CSWWCWDD74 pKa = 3.44YY75 pKa = 11.72EE76 pKa = 4.58VAFFGNNKK84 pKa = 9.52CVEE87 pKa = 4.23CDD89 pKa = 3.52PRR91 pKa = 11.84DD92 pKa = 3.72EE93 pKa = 5.41DD94 pKa = 5.54DD95 pKa = 3.6EE96 pKa = 4.51

Molecular weight: 10.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y5P1T1|A0A4Y5P1T1_9CAUD Uncharacterized protein OS=Pantoea phage vB_PagS_AAS21 OX=2575261 GN=AAS21_gp183 PE=4 SV=1
MM1 pKa = 8.12RR2 pKa = 11.84KK3 pKa = 9.36RR4 pKa = 11.84KK5 pKa = 9.33YY6 pKa = 8.93VQNRR10 pKa = 11.84YY11 pKa = 9.65INVQIRR17 pKa = 11.84CIFLVRR23 pKa = 11.84GMCKK27 pKa = 9.85SDD29 pKa = 3.21GLLGSEE35 pKa = 3.82NVVRR39 pKa = 11.84DD40 pKa = 3.44SRR42 pKa = 11.84IWFF45 pKa = 3.91

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

213

0

213

35046

19

1233

164.5

18.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.824 ± 0.381

1.093 ± 0.088

5.875 ± 0.145

6.705 ± 0.229

4.166 ± 0.152

6.317 ± 0.259

1.769 ± 0.106

6.6 ± 0.178

6.745 ± 0.226

8.52 ± 0.209

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.674 ± 0.111

5.347 ± 0.168

3.438 ± 0.126

3.578 ± 0.175

4.588 ± 0.12

6.549 ± 0.164

6.146 ± 0.226

6.714 ± 0.15

1.23 ± 0.069

4.12 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski